scholarly journals Somaclonal Variation in Habanero Pepper (Capsicum chinense Jacq.) as Assessed ISSR Molecular Markers

HortScience ◽  
2014 ◽  
Vol 49 (4) ◽  
pp. 481-485 ◽  
Author(s):  
Jericó J. Bello-Bello ◽  
Lourdes G. Iglesias-Andreu ◽  
Susana A. Avilés-Viñas ◽  
Eunice Gómez-Uc ◽  
Adriana Canto-Flick ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Issr Markers ◽  
Direct Organogenesis ◽  
Capsicum Chinense ◽  
Distance Analysis ◽  
System A ◽  
Regenerated Plants ◽  
Habanero Pepper ◽  
Genetic Distance Analysis

Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embryogenic callus system. A cluster analysis was performed to express in the form of dendrogram the relationships among different regeneration systems and the genetic variability detected. Genetic distance analysis indicated that our regeneration protocols are inappropriate for micropropagation, conservation, or genetic transformation; however, they may be applicable to breeding. This is the first report on the use of molecular analysis to evaluate genetic variation of in vitro-regenerated plants of Habanero pepper using ISSR markers.

scholarly journals Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1093-1098 ◽  
Author(s):  
Jericó J. Bello-Bello ◽  
Adriana Canto-Flick ◽  
Eduardo Balam-Uc ◽  
Eunice Gómez-Uc ◽  
Manuel L. Robert ◽  
...  
Keyword(s):  
Shoot Elongation ◽  
Nodal Segments ◽  
High Survival ◽  
Shoot Induction ◽  
Capsicum Chinense ◽  
Temporary Immersion Bioreactor ◽  
In Vitro Proliferation ◽  
Temporary Immersion ◽  
Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

Genetic Differentiation in Natural Populations of Grevillea robusta

1997 ◽  
Vol 45 (4) ◽  
pp. 669 ◽  
Author(s):  
C. E. Harwood ◽  
G. F. Moran ◽  
J. C. Bell
Keyword(s):  
Genetic Variation ◽  
Genetic Differentiation ◽  
Natural Populations ◽  
Grevillea Robusta ◽  
Distance Analysis ◽  
Riverine Habitat ◽  
Natural Range ◽  
Isozyme Loci ◽  
The Difference ◽  
Genetic Distance Analysis

Genetic variation in 23 natural populations of Grevillea robusta A.Cunn. from across the natural range of the species was examined using 20 isozyme loci assayed in young seedlings. Mean expected heterozygosity per population, He , varied from 0.080 to 0.131 with an average of 0.105. The genetic diversity of individual populations did not appear to be related to their ecological characteristics (araucarian vine forest or riverine habitat types) or their geographic locations. A genetic distance analysis indicated a significant separation of the populations into two regional groups, eight from the northern part of the natural range and the remaining 15 from central and southern areas. Between-population differences accounted for 17.9% of the total genetic variation, one-third of which was attributable to the difference between the two regional groups. Most alleles at the 20 loci occurred across most or all of the geographic range. This, and the low level of genetic differentiation between populations, suggest that genetic exchange between populations has been maintained, despite the pattern of natural distribution of the species in small, separated populations.

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

scholarly journals Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers

2021 ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Similarity Index ◽  
Issr Markers ◽  
Ficus Carica ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Inter Simple Sequence Repeats ◽  
Dna Primers

Abstract In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

scholarly journals Assessment of genetic diversity of Canthium parviflorum Lam by RAPD and ISSR markers

2017 ◽  
Vol 6 (11) ◽  
pp. 1775 ◽  
Author(s):  
Kokkanti Mallikarjuna ◽  
Chandra Kala Sirigiri ◽  
Ratnakar Reddi K.V.N. ◽  
Chandra Sekhar Akila ◽  
Obul Reddy Puli Chandra
Keyword(s):  
Genetic Diversity ◽  
Leaf Explants ◽  
Issr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Naturally Occurring ◽  
Regenerated Plants ◽  
The Impact ◽  
Rapd And Issr

Canthium parviflorum Lam is an important medicinal plant widely used in traditional systems of medicine with propagation limitations. In the present work, we are reporting the genetic diversity analysis of naturally occurring and in vitro grown plants by RAPD and ISSR markers. The plants developed on MS medium supplemented with BA (2mg/l) and NAA (0.5mg/l) using nodal and leaf explants were used along with plants present in five different geographical areas. Genetic diversity analysis using DNA based markers, RAPD and ISSR indicated that considerable genetic variations are present in naturally occurring plants. It is also indicated that tissue culture plants and their wild relatives show genetic similarity by grouping into one clad. The amplification products of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. The variations observed in naturally occurring plants could be due to the impact of local environmental factors and accumulation of mutations in the course of evolution. This is the first report on genetic diversity of Canthium plant populations.

scholarly journals Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

2021 ◽  
Vol 27 (4) ◽  
pp. 505-515
Author(s):  
Narges Asadi ◽  
Hossein Zarei ◽  
Seyyed Hamidreza Hashemi-Petroudi ◽  
Seyyed Javad Mousavizadeh
Keyword(s):  
Genetic Variation ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Somaclonal Variation ◽  
Growth Regulators ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plantlets ◽  
Regenerated Plants ◽  
High Concentrations

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

scholarly journals In Vitro Germplasm Conservation of Habanero Pepper (Capsicum chinense Jacq.)

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1247-1252
Author(s):  
María del C. Montalvo-Peniche ◽  
Lourdes G. Iglesias-Andreu ◽  
Javier O. Mijangos-Cortés ◽  
Sara L. Nahuat-Dzib ◽  
Felipe Barahona-Pérez ◽  
...  
Keyword(s):  
Culture Media ◽  
Germplasm Conservation ◽  
Nitrogen Sources ◽  
Capsicum Chinense ◽  
Minimal Growth ◽  
Habanero Pepper ◽  
Plantlet Quality ◽  
In Vitro Germplasm Conservation ◽  
Media Concentration

To determine the effect of different nitrogen sources and osmotic regulators on minimal growth of Habanero pepper (Capsicum chinense Jacq.) germplasm for in vitro conservation, different concentrations of nitrate, sucrose, mannitol, and sorbitol were evaluated. The micropropagation system based on Santana-Buzzy et al. (2006) culture medium was modified in its nitrate concentrations: reduced to 50% and increased to 150%, and osmoregulators were added to the basal culture media: sucrose (6% and 8%), mannitol (2%, 4%, and 8%), or sorbitol (2%, 4%, and 8%). The apical meristems of germinated plants were cultivated in the different treatments for 35 weeks without subculture. Results have demonstrated that mannitol at 2% had the better effect on minimal growth of the plantlets and did not affect the plant physiology and quality. The plantlets remained small in size, turgent, with green leaves and stems and looked like normal plants until to the end of the evaluation period. Changes in nitrogen media concentration did not prove to be adequate for conserving because they affected the plantlet quality (they became chlorotic). The presence of sorbitol and high osmolite concentrations induced minimal growth but reduced the plant quality. Sucrose at mid or low concentrations did not induce minimal growth.

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