scholarly journals Mutagenesis of in vitro cultures of Miscanthus × giganteus cultivar Freedom and detecting polymorphisms of regenerated plants using ISSR markers

2015 ◽  
Vol 65 ◽  
pp. 110-116 ◽  
Author(s):  
Dinum Perera ◽  
Daniel J. Barnes ◽  
Brian S. Baldwin ◽  
Nancy A. Reichert
Keyword(s):  
Issr Markers ◽  
In Vitro Cultures ◽  
Miscanthus Giganteus ◽  
Regenerated Plants

scholarly journals The effect of cytokinins on shoot proliferation, biochemical changes and genetic stability of Rhododendron ‘Kazimierz Odnowiciel’ in the in vitro cultures

2021 ◽  
Author(s):  
Karolina Nowakowska ◽  
Anna Pińkowska ◽  
Ewa Siedlecka ◽  
Andrzej Pacholczak
Keyword(s):  
Efficient Method ◽  
Plant Material ◽  
Soluble Sugars ◽  
Shoot Proliferation ◽  
Issr Markers ◽  
In Vitro Cultures ◽  
Biochemical Changes ◽  
Stock Plant

AbstractShoot proliferation is a very important micropropagation phase, decisive for economic efficiency of this method for a given taxon. To obtain a high multiplication ratio and a good quality of microshoots a detailed propagation protocol must be developed for particular species or even cultivars. Rhododendron ‘Kazimierz Odnowiciel’ is a relatively new cultivar distinguished by large, beautiful flowers and high frost resistance so there is a need to develop an efficient method of its propagation to satisfy a growing demand for this plant. The aim of the experiment was to evaluate effects of cytokinins: meta-Topolin (mT), zeatin (ZEA), 6-benzyladenine (BA), thidiazuron (TDZ), 2-isopentenyladenine (2iP), or the combination of 2iP+ZEA on proliferation of shoots in R. ‘Kazimierz Odnowiciel’ cultured on Anderson’s medium (AN). Biochemical changes in plant material affected by cytokinins during this phase of micropropagation were determined and occurrence of genetical changes was followed using ISSR markers. TDZ, ZEA or the combination of ZEA+2iP resulted in 100% explant regeneration. On the medium with TDZ or ZEA over two new shoots per explant were produced but the highest proliferation was attained on the medium containing ZEA+2iP – over three shoots per explant. Microshoots developed in this treatment had also the highest contents of chlorophyll, carotenoids and soluble sugars as well as the highest catalase activity. Microshoots formed on the medium with zeatin showed the lowest polymorphism (below 4%) relative to a stock plant.

scholarly journals The effect of media composition on multiplication of grape rootstocks in vitro

2012 ◽  
Vol 60 (8) ◽  
pp. 141-144 ◽  
Author(s):  
Břetislav Křižan ◽  
Eva Ondrušiková ◽  
Jana Moudrá
Keyword(s):  
Mass Production ◽  
Plant Material ◽  
In Vitro Cultures ◽  
Media Composition ◽  
Mass Propagation ◽  
Regenerated Plants ◽  
Optimized Protocol ◽  
Embryogenic Calluses ◽  
Murashige Skoog

The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

scholarly journals Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers

2021 ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Similarity Index ◽  
Issr Markers ◽  
Ficus Carica ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Inter Simple Sequence Repeats ◽  
Dna Primers

Abstract In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

scholarly journals Assessment of genetic diversity of Canthium parviflorum Lam by RAPD and ISSR markers

2017 ◽  
Vol 6 (11) ◽  
pp. 1775 ◽  
Author(s):  
Kokkanti Mallikarjuna ◽  
Chandra Kala Sirigiri ◽  
Ratnakar Reddi K.V.N. ◽  
Chandra Sekhar Akila ◽  
Obul Reddy Puli Chandra
Keyword(s):  
Genetic Diversity ◽  
Leaf Explants ◽  
Issr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Naturally Occurring ◽  
Regenerated Plants ◽  
The Impact ◽  
Rapd And Issr

Canthium parviflorum Lam is an important medicinal plant widely used in traditional systems of medicine with propagation limitations. In the present work, we are reporting the genetic diversity analysis of naturally occurring and in vitro grown plants by RAPD and ISSR markers. The plants developed on MS medium supplemented with BA (2mg/l) and NAA (0.5mg/l) using nodal and leaf explants were used along with plants present in five different geographical areas. Genetic diversity analysis using DNA based markers, RAPD and ISSR indicated that considerable genetic variations are present in naturally occurring plants. It is also indicated that tissue culture plants and their wild relatives show genetic similarity by grouping into one clad. The amplification products of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. The variations observed in naturally occurring plants could be due to the impact of local environmental factors and accumulation of mutations in the course of evolution. This is the first report on genetic diversity of Canthium plant populations.

Genotoxic effect of Pb and Cd on in vitro cultures of Sphagnum palustre : An evaluation by ISSR markers

Chemosphere ◽  
2017 ◽  
Vol 181 ◽  
pp. 208-215 ◽  
Author(s):  
Maria Cristina Sorrentino ◽  
Fiore Capozzi ◽  
Simonetta Giordano ◽  
Valeria Spagnuolo
Keyword(s):  
Issr Markers ◽  
In Vitro Cultures ◽  
Genotoxic Effect ◽  
Sphagnum Palustre

scholarly journals Somaclonal Variation in Habanero Pepper (Capsicum chinense Jacq.) as Assessed ISSR Molecular Markers

HortScience ◽  
2014 ◽  
Vol 49 (4) ◽  
pp. 481-485 ◽  
Author(s):  
Jericó J. Bello-Bello ◽  
Lourdes G. Iglesias-Andreu ◽  
Susana A. Avilés-Viñas ◽  
Eunice Gómez-Uc ◽  
Adriana Canto-Flick ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Issr Markers ◽  
Direct Organogenesis ◽  
Capsicum Chinense ◽  
Distance Analysis ◽  
System A ◽  
Regenerated Plants ◽  
Habanero Pepper ◽  
Genetic Distance Analysis

Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embryogenic callus system. A cluster analysis was performed to express in the form of dendrogram the relationships among different regeneration systems and the genetic variability detected. Genetic distance analysis indicated that our regeneration protocols are inappropriate for micropropagation, conservation, or genetic transformation; however, they may be applicable to breeding. This is the first report on the use of molecular analysis to evaluate genetic variation of in vitro-regenerated plants of Habanero pepper using ISSR markers.

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