Determination of Sulfhydryl Groups

1963 ◽  
pp. 21-51 ◽  
Author(s):  
Herbert Stern
Keyword(s):  
Sulfhydryl Groups

scholarly journals Determination of Exposed Sulfhydryl Groups in Heated β-Lactoglobulin A Using IAEDANS and Mass Spectrometry

2007 ◽  
Vol 55 (17) ◽  
pp. 7107-7113 ◽  
Author(s):  
Joseph J. Kehoe ◽  
André Brodkorb ◽  
Daniel Mollé ◽  
Emilie Yokoyama ◽  
Marie-Héléne Famelart ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sulfhydryl Groups ◽  
Β Lactoglobulin

scholarly journals Determination of Sulfhydryl Groups in Milk Using Ellman's Procedure and Clarifying Reagent®

1993 ◽  
Vol 76 (8) ◽  
pp. 2152-2155 ◽  
Author(s):  
Marie-France Guingamp ◽  
Gerard Humbert ◽  
Guy Linden
Keyword(s):  
Sulfhydryl Groups

scholarly journals Combined quantitative cytophotometric method for staining indolyl and sulfhydryl groups and protein.

1983 ◽  
Vol 31 (7) ◽  
pp. 967-970 ◽  
Author(s):  
Y Nakae ◽  
M Shono ◽  
H Ishizuka
Keyword(s):  
Sulfhydryl Groups ◽  
Brilliant Blue ◽  
Quantitative Histochemistry ◽  
Coomassie Brilliant Blue ◽  
Protein Staining ◽  
Coomassie Brilliant

Sulfenylation with 2-nitrophenylsulfenyl chloride (NPS-Cl), which is specific for tryptophyl and cysteinyl residues in protein, was applied to quantitative histochemistry. By measurement of the absorbance values at 370 nm of sections stained with NPS-Cl, Beer-Lambert's law was found to hold for NPS staining. Treatment of NPS-stained sections with 2-mercaptoethanol (ME) (NPS-ME staining) resulted in sulfenylation of tryptophyl residues only. For determination of the amounts of tryptophyl and cysteinyl residues per unit of protein, protein staining with Coomassie Brilliant Blue (CB) was combined with NPS and NPS-ME staining. CB and NPS-CBB staining also followed Beer-Lambert's law. By measuring the absorbance values at 370 and 650 nm of doubly stained sections, the relative contents of tryptophyl and cysteinyl residues in various tissue proteins were calculated. This method will be useful for the investigation of changes in both protein amount and composition.

FREE SULFHYDRYL GROUPS IN HAPTOGLOBIN

1967 ◽  
Vol 45 (4) ◽  
pp. 551-556 ◽  
Author(s):  
Brenda L. Tattrie ◽  
G. E. Connell
Keyword(s):  
Acid Hydrolysis ◽  
Guanidine Hydrochloride ◽  
Spectrophotometric Titration ◽  
Sulfhydryl Groups

Human haptoglobin of types 1-1 and 2-2 has been examined for its content of free sulfhydryl groups by two methods: (i) treatment of haptoglobin in the presence of 7.2 M guanidine hydrochloride with 14C-iodoacetate followed by acid hydrolysis and determination of 14C-carboxymethylcysteine content, and (ii) spectrophotometric titration of haptoglobin in the presence of 7.2 M guanidine hydrochloride with p-chloromercuribenzoate. By both methods, the content of free sulfhydryl was less than 1 equiv/105 g of protein.

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