Generation of Naive Human Antibody Libraries

2001 ◽  
pp. 93-108 ◽  
Author(s):  
Catherine Hutchings ◽  
Sara Carmen ◽  
Simon Lennard
Keyword(s):  
Human Antibody ◽  
Antibody Libraries

Human antibody libraries

1996 ◽  
Vol 2 (1) ◽  
pp. 72-73
Author(s):  
T.J. Vaughan ◽  
A.J. Williams ◽  
K. Pritchard ◽  
J.K. Osbourn ◽  
A.R. Pope ◽  
...  
Keyword(s):  
Human Antibody ◽  
Antibody Libraries

scholarly journals An improved yeast transformation method for the generation of very large human antibody libraries

2010 ◽  
Vol 23 (4) ◽  
pp. 155-159 ◽  
Author(s):  
Lorenzo Benatuil ◽  
Jennifer M. Perez ◽  
Jonathan Belk ◽  
Chung-Ming Hsieh
Keyword(s):  
Transformation Method ◽  
Human Antibody ◽  
Yeast Transformation ◽  
Antibody Libraries

scholarly journals Generation of “LYmph Node Derived Antibody Libraries” (LYNDAL) for selecting fully human antibody fragments with therapeutic potential

mAbs ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 130-142 ◽  
Author(s):  
Philipp Diebolder ◽  
Armin Keller ◽  
Stephanie Haase ◽  
Anne Schlegelmilch ◽  
Jonathan D Kiefer ◽  
...  
Keyword(s):  
Lymph Node ◽  
Therapeutic Potential ◽  
Antibody Fragments ◽  
Human Antibody ◽  
Antibody Libraries

Human antibody libraries: A race to engineer and explore a larger diversity

10.2741/2749 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 1117 ◽  
Author(s):  
Philippe Mondon
Keyword(s):  
Human Antibody ◽  
Antibody Libraries

scholarly journals Transferring the Characteristics of Naturally Occurring and Biased Antibody Repertoires to Human Antibody Libraries by Trapping CDRH3 Sequences

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43471 ◽  
Author(s):  
Sophie Venet ◽  
Ulla Ravn ◽  
Vanessa Buatois ◽  
Franck Gueneau ◽  
Sébastien Calloud ◽  
...  
Keyword(s):  
Human Antibody ◽  
Naturally Occurring ◽  
Antibody Libraries ◽  
Antibody Repertoires

scholarly journals Functional selection of protease inhibitory antibodies

2019 ◽  
Vol 116 (33) ◽  
pp. 16314-16319 ◽  
Author(s):  
Tyler Lopez ◽  
Zahid Mustafa ◽  
Chuan Chen ◽  
Ki Baek Lee ◽  
Aaron Ramirez ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Peptide Sequence ◽  
Great Promise ◽  
Human Antibody ◽  
Cellular Assays ◽  
Antibody Discovery ◽  
Antibody Libraries ◽  
Inhibitory Antibodies ◽  
Cleavable Peptide ◽  
Inhibition Potency

Critical for diverse biological processes, proteases represent one of the largest families of pharmaceutical targets. To inhibit pathogenic proteases with desired selectivity, monoclonal antibodies (mAbs) hold great promise as research tools and therapeutic agents. However, identification of mAbs with inhibitory functions is challenging because current antibody discovery methods rely on binding rather than inhibition. This study developed a highly efficient selection method for protease inhibitory mAbs by coexpressing 3 recombinant proteins in the periplasmic space of Escherichia coli—an antibody clone, a protease of interest, and a β-lactamase modified by insertion of a protease cleavable peptide sequence. During functional selection, inhibitory antibodies prevent the protease from cleaving the modified β-lactamase, thereby allowing the cell to survive in the presence of ampicillin. Using this method to select from synthetic human antibody libraries, we isolated panels of mAbs inhibiting 5 targets of 4 main protease classes: matrix metalloproteinases (MMP-14, a predominant target in metastasis; MMP-9, in neuropathic pain), β-secretase 1 (BACE-1, an aspartic protease in Alzheimer’s disease), cathepsin B (a cysteine protease in cancer), and Alp2 (a serine protease in aspergillosis). Notably, 37 of 41 identified binders were inhibitory. Isolated mAb inhibitors exhibited nanomolar potency, exclusive selectivity, excellent proteolytic stability, and desired biological functions. Particularly, anti-Alp2 Fab A4A1 had a binding affinity of 11 nM and inhibition potency of 14 nM, anti-BACE1 IgG B2B2 reduced amyloid beta (Aβ40) production by 80% in cellular assays, and IgG L13 inhibited MMP-9 but not MMP-2/-12/-14 and significantly relieved neuropathic pain development in mice.

Human antibody libraries in Escherichia coli

1995 ◽  
Vol 41 (2-3) ◽  
pp. 187-195 ◽  
Author(s):  
Melvyn Little ◽  
Frank Breitling ◽  
Stefan Dübel ◽  
Patrick Fuchs ◽  
Michael Braunagel
Keyword(s):  
Escherichia Coli ◽  
Human Antibody ◽  
Antibody Libraries
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