Methods, applications and concepts of metabolite profiling: Primary metabolism

2007 ◽  
pp. 171-194 ◽  
Author(s):  
Dirk Steinhauser ◽  
Joachim Kopka
Keyword(s):  
Metabolite Profiling ◽  
Primary Metabolism

scholarly journals Involvement of SUT1 and SUT2 Sugar Transporters in the Impairment of Sugar Transport and Changes in Phloem Exudate Contents in Phytoplasma-Infected Plants

2021 ◽  
Vol 22 (2) ◽  
pp. 745
Author(s):  
Federica De Marco ◽  
Brigitte Batailler ◽  
Michael R. Thorpe ◽  
Frédérique Razan ◽  
Rozenn Le Hir ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Sugar Transport ◽  
Starch Accumulation ◽  
Primary Metabolism ◽  
Phloem Exudate ◽  
Phloem Sap ◽  
Sugar Transporters ◽  
Abnormal Growth ◽  
Sucrose Transporters

Phytoplasmas inhabit phloem sieve elements and cause abnormal growth and altered sugar partitioning. However, how they interact with phloem functions is not clearly known. The phloem responses were investigated in tomatoes infected by “Candidatus Phytoplasma solani” at the beginning of the symptomatic stage, the first symptoms appearing in the newly emerged leaf at the stem apex. Antisense lines impaired in the phloem sucrose transporters SUT1 and SUT2 were included. In symptomatic sink leaves, leaf curling was associated with higher starch accumulation and the expression of defense genes. The analysis of leaf midribs of symptomatic leaves indicated that transcript levels for genes acting in the glycolysis and peroxisome metabolism differed from these in noninfected plants. The phytoplasma also multiplied in the three lower source leaves, even if it was not associated with the symptoms. In these leaves, the rate of phloem sucrose exudation was lower for infected plants. Metabolite profiling of phloem sap-enriched exudates revealed that glycolate and aspartate levels were affected by the infection. Their levels were also affected in the noninfected SUT1- and SUT2-antisense lines. The findings suggest the role of sugar transporters in the responses to infection and describe the consequences of impaired sugar transport on the primary metabolism.

Ion mobility spectrometry in metabolite profiling of complex plant extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
PJ Eugster ◽  
R Knochenmuss ◽  
JL Wolfender
Keyword(s):  
Ion Mobility Spectrometry ◽  
Plant Extracts ◽  
Ion Mobility ◽  
Metabolite Profiling

Screening of some Asteraceae to discover new active compounds against Trypanosoma brucei by metabolite profiling and PLS analysis

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
MS Nogueira ◽  
FB da Costa ◽  
MA Magenta ◽  
M Kaiser ◽  
R Brun ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Metabolite Profiling ◽  
Active Compounds ◽  
Pls Analysis

1H NMR based metabolite profiling with special focus on tropane alkaloids in Duboisia species

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
SF Ullrich ◽  
A Rothauer ◽  
O Kayser
Keyword(s):  
1H Nmr ◽  
Metabolite Profiling ◽  
Tropane Alkaloids ◽  
Special Focus
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