Hepatitis C Virus Nonstructural Region 5A Protein: A Potent Transcriptional Activator

1999 ◽  
pp. 45-58 ◽  
Author(s):  
Naoya Kato ◽  
Keng-Hsin Lan ◽  
Suzane Kioko Ono-Nita ◽  
Hideo Yoshida ◽  
Yasushi Shiratori ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein A ◽  
Transcriptional Activator

Hepatitis C virus nonstructural region 5A protein is a potent transcriptional activator.

1997 ◽  
Vol 71 (11) ◽  
pp. 8856-8859 ◽  
Author(s):  
N Kato ◽  
K H Lan ◽  
S K Ono-Nita ◽  
Y Shiratori ◽  
M Omata
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Transcriptional Activator

scholarly journals Interactions between Hepatitis C Virus and the Human Apolipoprotein H Acute Phase Protein: A Tool for a Sensitive Detection of the Virus

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140900 ◽  
Author(s):  
Ilias Stefas ◽  
Sylvia Tigrett ◽  
Grégor Dubois ◽  
Marco Kaiser ◽  
Estelle Lucarz ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Protein A ◽  
Sensitive Detection ◽  
Human Apolipoprotein ◽  
Apolipoprotein H ◽  
Phase Protein

Myxovirus resistance protein A inhibits hepatitis C virus replication through JAK-STAT pathway activation

2018 ◽  
Vol 163 (6) ◽  
pp. 1429-1438 ◽  
Author(s):  
Hailong Wang ◽  
Xiu Xin ◽  
Mingzhen Wang ◽  
Lingling Han ◽  
Jiadai Li ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Protein A ◽  
Pathway Activation ◽  
Resistance Protein ◽  
Stat Pathway

scholarly journals The Interaction of Viperin with Hepatitis C Virus Non-Structural Protein 5A Inhibits the Catalytic Activity of Viperin

2019 ◽  
Author(s):  
Soumi Ghosh ◽  
Ayesha M. Patel ◽  
Timothy J. Grunkemeyer ◽  
Arti B. Dumbrepatil ◽  
Kelcie Zegalia ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Specific Activity ◽  
Regulatory Protein ◽  
Protein A ◽  
Structural Protein ◽  
Radical Sam ◽  
Replication Complex ◽  
Wide Range ◽  
Radical Sam Enzyme

AbstractThe radical SAM enzyme viperin exerts a wide range of antiviral effects through both the synthesis of the antiviral nucleotide 3’-deoxy-3’, 4’-didehydro-CTP (ddhCTP) and through its interactions with various cellular and viral proteins. Here we investigate the interaction of viperin with hepatitis C virus non-structural protein 5A (NS5A) and the host sterol regulatory protein, vesicle-associated membrane protein A (VAP-33). NS5A and VAP-33 form part of the viral replication complex that is essential for copying the RNA genome of the virus. Using transfected enzymes in HEK293T cells, we show that viperin binds to both NS5A and VAP-33 independently and that this interaction is dependent on all three proteins being localized to the ER membrane. Co-expression of viperin with VAP-33 and NS5A was found to reduce NS5A levels, most likely by increasing the rate of proteasomal degradation. However, co-expression of viperin with VAP-33 and NS5A also reduces the specific activity of viperin by ~ 3-fold. This observation suggests that NS5A may have evolved to bind viperin as a strategy to reduce ddhCTP synthesis, thereby reducing possibility of the replication complex introducing this chain-terminating nucleotide during genome synthesis.

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