Genetics of C1 metabolism regulation in Paracoccus denitrificans

1996 ◽  
pp. 126-132 ◽  
Author(s):  
N. Harms ◽  
J. Ras ◽  
S. Koning ◽  
W. N. M. Reijnders ◽  
A. H. Stouthamer ◽  
...  
Keyword(s):  
Paracoccus Denitrificans ◽  
C1 Metabolism ◽  
Metabolism Regulation

Physiological role of S-formylglutathione hydrolase in C1 metabolism of the methylotrophic yeast Candida boidinii

Microbiology ◽  
2003 ◽  
Vol 149 (8) ◽  
pp. 1971-1979 ◽  
Author(s):  
Hiroya Yurimoto ◽  
Bumjun Lee ◽  
Taisuke Yano ◽  
Yasuyoshi Sakai ◽  
Nobuo Kato
Keyword(s):  
Carbon Source ◽  
Paracoccus Denitrificans ◽  
Physiological Role ◽  
Methylotrophic Yeast ◽  
Candida Boidinii ◽  
Predicted Amino Acid Sequence ◽  
Type I ◽  
C1 Metabolism ◽  
Oxidation Pathway ◽  
Formaldehyde Oxidation

The methylotrophic yeast Candida boidinii exhibits S-formylglutathione hydrolase activity (FGH, EC 3.1.2.12), which is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source. The structural gene, FGH1, was cloned from C. boidinii, and its predicted amino acid sequence showed more than 60 % similarity to those of FGHs from Paracoccus denitrificans and Saccharomyces cerevisiae, and human esterase D. FGH from C. boidinii contained a C-terminal tripeptide, SKL, which is a type I peroxisome-targeting signal, and a bimodal distribution of FGH between peroxisomes and the cytosol was demonstrated. The FGH1 gene was disrupted in the C. boidinii genome by one-step gene disruption. The fgh1Δ strain was still able to grow on methanol as a carbon source under methanol-limited chemostat conditions with low dilution rates (D<0·05 h−1), conditions under which a strain with disruption of the gene for formaldehyde dehydrogenase (another enzyme involved in the formaldehyde oxidation pathway) could not survive. These results suggested that FGH is not essential but necessary for optimal growth on methanol. This is believed to be the first report of detailed analyses of the FGH1 gene in a methylotrophic yeast strain.

Coping with formaldehyde during C1 metabolism of Paracoccus denitrificans

2000 ◽  
Vol 8 (1-3) ◽  
pp. 37-50 ◽  
Author(s):  
Rob J.M. Van Spanning ◽  
Simon de Vries ◽  
Nellie Harms
Keyword(s):  
Paracoccus Denitrificans ◽  
C1 Metabolism

Development of Experimental and Theoretical Methodology for Determination of Characteristics of central Metabolism Regulation in Cancer Cells

2012 ◽  
Vol 120 (10) ◽  
Author(s):  
D Shaumarova ◽  
A Benito ◽  
J Centelles ◽  
S Marin Martinez ◽  
V Selivanov ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Central Metabolism ◽  
Metabolism Regulation

Circadian model of carbohydrate metabolism regulation in normal

2016 ◽  
Vol 18 (4) ◽  
pp. 38-42
Author(s):  
V.P. Kitcyshin ◽  
◽  
V.V. Salukhov ◽  
T.A. Demidova ◽  
R.T. Sardinov ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Metabolism Regulation

Endocannabinoids

2018 ◽  
Author(s):  
Leslie Iversen
Keyword(s):  
Arachidonic Acid ◽  
Energy Metabolism ◽  
Pain Sensitivity ◽  
Large Family ◽  
Cardiovascular Control ◽  
Lipid Signaling ◽  
Physiological Functions ◽  
Functional Roles ◽  
Metabolism Regulation ◽  
Mediators Of Inflammation

The endocannabinoids are part of a large family of lipid signaling molecules derived from arachidonic acid, including the prostaglandins and leukotrienes, which are important mediators of inflammation. Far less is known about the newer members of the endocannabinoid group, and it remains unclear whether they all play important functional roles. This chapter reviews the multiple members of this family and their biosynthesis and inactivation. Physiological functions, including retrograde synaptic signaling, control of energy metabolism, regulation of pain sensitivity, and cardiovascular control, are discussed. In addition, the chapter reports the synthesis of novel agonists, antagonists, and compounds inhibiting endocannabinoid inactivation as novel medicines.

Sign in / Sign up

Export Citation Format

Share Document