Cloning and Characterization of Trichoderma Harzianum Genes Induced During Growth on Rhizoctonia Solani Cell Walls

1996 ◽  
pp. 133-137
Author(s):  
Valerie Vasseur ◽  
Marc Van Montagu ◽  
Gustavo H. Goldman
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Cell Walls

scholarly journals Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls

2015 ◽  
Vol 42 (2) ◽  
pp. 10-10 ◽  
Author(s):  
Rubén Polanco ◽  
Constanza Pino ◽  
Ximena Besoain ◽  
Jaime Montealegre ◽  
Luz M Pérez
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Cell Walls ◽  
Mutant Strains ◽  
Enhanced Secretion

scholarly journals Trichoderma harzianum genes induced during growth on Rhizoctonia solani cell walls

Microbiology ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 767-774 ◽  
Author(s):  
V. Vasseur ◽  
M. Van Montagu ◽  
G. H. Goldman
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Cell Walls

scholarly journals Role of the Trichoderma harzianumEndochitinase Gene, ech42, in Mycoparasitism

1999 ◽  
Vol 65 (3) ◽  
pp. 929-935 ◽  
Author(s):  
Carolina Carsolio ◽  
Nicole Benhamou ◽  
Shoshan Haran ◽  
Carlos Cortés ◽  
Ana Gutiérrez ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Cell Walls ◽  
Sclerotium Rolfsii ◽  
Biocontrol Agents ◽  
Wild Type ◽  
Greenhouse Experiments ◽  
Multiple Copies

ABSTRACT The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased up to 42-fold in multicopy strains as compared with the wild type, whereas gene disruptants exhibited practically no activity. The densities of chitin labeling of Rhizoctonia solani cell walls, after interactions with gene disruptants were not statistically significantly different than the density of chitin labeling after interactions with the wild type. Finally, no major differences in the efficacies of the strains generated as biocontrol agents against R. solani or Sclerotium rolfsii were observed in greenhouse experiments.

Characterization of machined polystyrene foam

1989 ◽  
Vol 47 ◽  
pp. 372-373
Author(s):  
C. W. Price ◽  
E. F. Lindsey ◽  
R. M. Franks ◽  
M. A. Lane
Keyword(s):  
Surface Finish ◽  
Cell Walls ◽  
Surface Structures ◽  
Efficient Technique ◽  
Low Density ◽  
Polystyrene Foam ◽  
Planar Surfaces ◽  
Machining Characteristics

Diamond-point turning is an efficient technique for machining low-density polystyrene foam, and the surface finish can be substantially improved by grinding. However, both diamond-point turning and grinding tend to tear and fracture cell walls and leave asperities formed by agglomerations of fragmented cell walls. Vibratoming is proving to be an excellent technique to form planar surfaces in polystyrene, and the machining characteristics of vibratoming and diamond-point turning are compared.Our work has demonstrated that proper evaluation of surface structures in low density polystyrene foam requires stereoscopic examinations; tilts of + and − 3 1/2 degrees were used for the stereo pairs. Coating does not seriously distort low-density polystyrene foam. Therefore, the specimens were gold-palladium coated and examined in a Hitachi S-800 FESEM at 5 kV.

Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls

2021 ◽  
Vol 69 (7) ◽  
pp. 2226-2235
Author(s):  
Greta Canelli ◽  
Patricia Murciano Martínez ◽  
Sean Austin ◽  
Mark E. Ambühl ◽  
Fabiola Dionisi ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Cell Walls ◽  
Morphological Characterization ◽  
Crypthecodinium Cohnii

Characterization of the major antifungal extrolite from rice endophyte Lysinibacillus sphaericus against Rhizoctonia solani

2021 ◽  
Author(s):  
S. Shabanamol ◽  
Meenu Thampi ◽  
P. Sajana ◽  
Sherin Varghese ◽  
S. Karthika ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Lysinibacillus Sphaericus

scholarly journals Characterization of Passage-Selected Vancomycin-Resistant Staphylococcus aureus Strains of Diverse Parental Backgrounds

2000 ◽  
Vol 44 (2) ◽  
pp. 294-303 ◽  
Author(s):  
Richard F. Pfeltz ◽  
Vineet K. Singh ◽  
Jennifer L. Schmidt ◽  
Michael A. Batten ◽  
Christopher S. Baranyk ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Walls ◽  
Methicillin Resistance ◽  
Surface Hydrophobicity ◽  
Whole Cell ◽  
Transmission Electron ◽  
Vancomycin Mics ◽  
Vancomycin Resistant ◽  
Doubling Times

ABSTRACT A series of 12 Staphylococcus aureus strains of various genetic backgrounds, methicillin resistance levels, and autolytic activities were subjected to selection for the glycopeptide-intermediate S. aureus (GISA) susceptibility phenotype on increasing concentrations of vancomycin. Six strains acquired the phenotype rapidly, two did so slowly, and four failed to do so. The vancomycin MICs for the GISA strains ranged from 4 to 16 μg/ml, were stable to 20 nonselective passages, and expressed resistance homogeneously. Neither ease of acquisition of the GISA phenotype nor the MIC attained correlated with methicillin resistance hetero- versus homogeneity or autolytic deficiency or sufficiency. Oxacillin MICs were generally unchanged between parent and GISA strains, although the mec members of both isogenic methicillin-susceptible and methicillin-resistant pairs acquired the GISA phenotype more rapidly and to higher MICs than did their susceptible counterparts. Transmission electron microscopy revealed that the GISA strains appeared normal in the absence of vancomycin but had thickened and diffuse cell walls when grown with vancomycin at one-half the MIC. Common features among GISAs were reduced doubling times, decreased lysostaphin susceptibilities, and reduced whole-cell and zymographic autolytic activities in the absence of vancomycin. This, with surface hydrophobicity differences, indicated that even in the absence of vancomycin the GISA cell walls differed from those of the parents. Autolytic activities were further reduced by the inclusion of vancomycin in whole-cell and zymographic studies. The six least vancomycin-susceptible GISA strains exhibited an increased capacity to remove vancomycin from the medium versus their parent lines. This study suggests that while some elements of the GISA phenotype are strain specific, many are common to the phenotype although their expression is influenced by genetic background. GISA strains with similar glycopeptide MICs may express individual components of the phenotype to different extents.

Characterization of genes encoding novel peptidases in the biocontrol fungus Trichoderma harzianum CECT 2413 using the TrichoEST functional genomics approach

2007 ◽  
Vol 51 (5) ◽  
pp. 331-342 ◽  
Author(s):  
M. Belén Suárez ◽  
J. Antonio Vizcaíno ◽  
Antonio Llobell ◽  
Enrique Monte
Keyword(s):  
Functional Genomics ◽  
Trichoderma Harzianum ◽  
Genes Encoding
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