Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls

2021 ◽  
Vol 69 (7) ◽  
pp. 2226-2235
Author(s):  
Greta Canelli ◽  
Patricia Murciano Martínez ◽  
Sean Austin ◽  
Mark E. Ambühl ◽  
Fabiola Dionisi ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Cell Walls ◽  
Morphological Characterization ◽  
Crypthecodinium Cohnii

scholarly journals Morphological characterization of seeds in Portulacaceae

Phytotaxa ◽  
2013 ◽  
Vol 141 (1) ◽  
pp. 1 ◽  
Author(s):  
Gilberto Ocampo
Keyword(s):  
Cell Walls ◽  
Individual Cell ◽  
Morphological Diversity ◽  
Morphological Characterization ◽  
Seed Morphology ◽  
Important Variation ◽  
Species Complexes ◽  
Multiple Samples

Portulacaceae is a monogeneric family with around 100 species distributed around the world. Seed morphology is diverse in this group, and its variation has been used for taxonomic and identification purposes. However, documentation of seed morphology has focused on a few species complexes or has been limited to specific countries and geographic areas. Moreover, the terminology employed in different investigations is heterogeneous, thus complicating analyses of seed morphological diversity in Portulacaceae. In this study, the seed morphology of 58 samples representing 49 species, 9 subspecies, and 2 cultivars of Portulaca was documented with the use of scanning electron microscopy (SEM). Based on the examination of the resulting images, a set of morphological features for describing the general shape of the seeds and their individual cell features is proposed; in addition, morphological descriptions for all taxa included in this study are provided. The results indicate that the shape of the seeds was constant within species, contrary to the characters that describe individual cell features, which showed important variation even within the same seed sample. In particular, the outline of the cells and the curvature of the anticlinal cell walls (ACW) were very variable, so a combination of character states was needed to describe the overall diversity of a sample. Conversely, the relief of the periclinal cell walls (PCW) was relatively constant throughout the seeds of the same species, but the relief of the cells of the lateral and peripheral faces was dissimilar in a number of taxa. In addition, other studies provide evidence that the relief of the PCW may vary in widely distributed species. Increased taxon sampling and multiple samples of species with wide geographical ranges will facilitate the study of patterns of variation, and may provide insights into the role of environmental variables on seed diversity of Portulacaceae.

scholarly journals Morphological characterization of Kielmeyera coriacea Mart. Cork from brazilian Cerrado

CERNE ◽  
2011 ◽  
Vol 17 (3) ◽  
pp. 387-392 ◽  
Author(s):  
Polliana D'Angelo Rios ◽  
Fábio Akira Mori ◽  
Ana Carolina Maioli Campos Barbosa
Keyword(s):  
Cell Walls ◽  
Morphological Characteristics ◽  
Quercus Suber ◽  
Morphological Characterization ◽  
Morphological Aspect ◽  
Brazilian Cerrado ◽  
Growth Season ◽  
Sem Images ◽  
Kielmeyera Coriacea

Kielmeyera coriacea Mart., known as "pau-santo", is mentioned in the literature as the main tree species which produces cork of the Brazilian Cerrado. The purpose of this study was to describe the morphological aspect of Kielmeyera coriacea ("pau-santo") cork cells through its microscopic structure and to compare it with the cellular morphology of Quercus suber (cork oak), which is the main cork producing species worldwide. The bark from three trees of the species Kielmeyera coriacea Mart. was collected randomly at points 1.30 m above the ground, with four repetitions per sample. Samples came from native stands situated in the region of Luminárias - MG, Brazil. The study of morphological characteristics was conducted by counting the number of sides of the cells, and measuring the dimensions of the cells from images obtained by scanning electron microscopy (SEM). Images were analyzed using the LEOUIF software. The distribution in faces and edges of cork cells from Kielmeyera coriacea, was observed to have from 4 to 8 sides, with a predominance of hexagonal cells, similar to Quercus suber. The averages of height and thickness of the cell walls were 40 to 70 µm and 1.5 to 2.0 µm respectively, indicating cells from an early growth season. Both height and thickness were observed to be greater than those present in Quercus suber.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Characterization of machined polystyrene foam

1989 ◽  
Vol 47 ◽  
pp. 372-373
Author(s):  
C. W. Price ◽  
E. F. Lindsey ◽  
R. M. Franks ◽  
M. A. Lane
Keyword(s):  
Surface Finish ◽  
Cell Walls ◽  
Surface Structures ◽  
Efficient Technique ◽  
Low Density ◽  
Polystyrene Foam ◽  
Planar Surfaces ◽  
Machining Characteristics

Diamond-point turning is an efficient technique for machining low-density polystyrene foam, and the surface finish can be substantially improved by grinding. However, both diamond-point turning and grinding tend to tear and fracture cell walls and leave asperities formed by agglomerations of fragmented cell walls. Vibratoming is proving to be an excellent technique to form planar surfaces in polystyrene, and the machining characteristics of vibratoming and diamond-point turning are compared.Our work has demonstrated that proper evaluation of surface structures in low density polystyrene foam requires stereoscopic examinations; tilts of + and − 3 1/2 degrees were used for the stereo pairs. Coating does not seriously distort low-density polystyrene foam. Therefore, the specimens were gold-palladium coated and examined in a Hitachi S-800 FESEM at 5 kV.

Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara
Keyword(s):  
Sri Lanka ◽  
Morphological Characterization

Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

scholarly journals Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal
Keyword(s):  
Ph Value ◽  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Nano Materials ◽  
Elongation At Break ◽  
Stable Suspension ◽  
Average Size ◽  
Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

scholarly journals Morphological characterization of the Apis mellifera drones in the Southern Urals

2020 ◽  
Vol 604 ◽  
pp. 012030
Author(s):  
N R Gazizova ◽  
A G Mannapov ◽  
V N Sattarov ◽  
V G Semenov ◽  
A I Skvortsov ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Southern Urals ◽  
Morphological Characterization ◽  
The Southern Urals

scholarly journals Structural and Morphological Characterization of Bio-templated Reduced Graphene Oxide and their Antibacterial Efficacy

2021 ◽  
Author(s):  
Smina Chappalathottil Sethumadhavan ◽  
Lalitha Pottail ◽  
S. C. Sharma ◽  
Akhila Chithambharan ◽  
Suhas Ballal
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Morphological Characterization ◽  
Antibacterial Efficacy ◽  
Reduced Graphene
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