Biochemical and Cytological Changes in Young Tobacco Pollen during in vitro Starvation in Relation to Pollen Embryogenesis

1990 ◽  
pp. 228-233 ◽  
Author(s):  
V. ŽárskÝ ◽  
L. Říhová ◽  
J. TupÝ
Keyword(s):  
Pollen Embryogenesis ◽  
Tobacco Pollen ◽  
Cytological Changes

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

Control of the developmental pathway of tobacco pollen in vitro

Planta ◽  
1986 ◽  
Vol 168 (4) ◽  
pp. 427-432 ◽  
Author(s):  
M. Kyo ◽  
H. Harada
Keyword(s):  
Developmental Pathway ◽  
Tobacco Pollen

Morphology of Cell Injury: An Approach to the EDIT Programme by the Use of Tobacco Pollen Tubes

2002 ◽  
Vol 30 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Udo Kristen ◽  
Natalie Bischoff ◽  
Saskia Lisboa ◽  
Enno Schirmer ◽  
Sören Witt ◽  
...  
Keyword(s):  
Tip Growth ◽  
Cell Injury ◽  
Cytosolic Calcium ◽  
Pollen Tubes ◽  
In Vitro Cytotoxicity ◽  
Toxic Compounds ◽  
In Vitro System ◽  
Tobacco Pollen ◽  
Different Types

Tobacco pollen tubes were used as a standard in vitro system to investigate cell growth aberrations caused by some of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme chemicals and other toxic compounds. Changes in cytoskeletal pattern were observed in the tube cells by using tubu-lin immunofluorescence and rhodamin–phalloidin fluorescence for the localisation of microtubules and actin filaments, respectively. Four different types of cell malformation were found: screw-like growth, isodiametric tip swelling, hook formation, and pollen grain enlargement. We suggest that these malformations resulted from an interference by the chemicals with the cytosolic calcium gradient which controls tip growth and the orientation of the pollen tube. The results may contribute to a general understanding of toxicity-based cell malformations.

CORRELATED STUDIES OF STEROID FORMATION IN VITRO, ASCORBIC ACID CONCENTRATIONS, AND CYTOLOGICAL CHANGES IN ADRENAL GLANDS OF RATS GIVEN HISTAMINE

1958 ◽  
Vol 36 (1) ◽  
pp. 571-574
Author(s):  
E. Schönbaum ◽  
W. G. Bruce Casselman
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Glands ◽  
Cytological Changes

In the adrenal glands of rats given histamine, there are significant correlations between the increase in steroid formation in vitro, and the depletion of ascorbic acid or cytological changes in Z. fasciculata and Z. reticularis indicative of stimulation.

In-vitro culture of tobacco pollen: gene expression and protein synthesis

1992 ◽  
Vol 5 (4) ◽  
pp. 304-309 ◽  
Author(s):  
M. M. A. Van Herpen ◽  
P. F. M. de Groot ◽  
J. A. M. Schrauwen ◽  
K. J. P. T. van den Heuvel ◽  
K. A. P. Weterings ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
In Vitro Culture ◽  
Tobacco Pollen

Cytological Evidence of Adrenocortical Activity In Vitro

1962 ◽  
Vol s3-103 (64) ◽  
pp. 447-449
Author(s):  
W. G. BRUCE CASSELMAN ◽  
E. SCHÖNBAUM
Keyword(s):  
Intact Animal ◽  
Adrenocorticotrophic Hormone ◽  
Adrenocortical Cells ◽  
Cytological Evidence ◽  
Adrenocortical Activity ◽  
Cytological Changes

When stimulated by adrenocorticotrophic hormone, adrenocortical cells in vitroundergo cytological changes similar to those previously described for the intact animal.

scholarly journals Antimicrobial Effects of Interferon-Inducible CXC Chemokines against Bacillus anthracis Spores and Bacilli

2009 ◽  
Vol 77 (4) ◽  
pp. 1664-1678 ◽  
Author(s):  
Matthew A. Crawford ◽  
Yinghua Zhu ◽  
Candace S. Green ◽  
Marie D. Burdick ◽  
Patrick Sanz ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Antimicrobial Activities ◽  
Antimicrobial Effects ◽  
Bacillus Anthracis Spores ◽  
Cytological Changes ◽  
Vitro Analysis ◽  
Insight Into ◽  
In Vitro Analysis

ABSTRACT Based on previous studies showing that host chemokines exert antimicrobial activities against bacteria, we sought to determine whether the interferon-inducible Glu-Leu-Arg-negative CXC chemokines CXCL9, CXCL10, and CXCL11 exhibit antimicrobial activities against Bacillus anthracis. In vitro analysis demonstrated that all three CXC chemokines exerted direct antimicrobial effects against B. anthracis spores and bacilli including marked reductions in spore and bacillus viability as determined using a fluorometric assay of bacterial viability and CFU determinations. Electron microscopy studies revealed that CXCL10-treated spores failed to undergo germination as judged by an absence of cytological changes in spore structure that occur during the process of germination. Immunogold labeling of CXCL10-treated spores demonstrated that the chemokine was located internal to the exosporium in association primarily with the spore coat and its interface with the cortex. To begin examining the potential biological relevance of chemokine-mediated antimicrobial activity, we used a murine model of inhalational anthrax. Upon spore challenge, the lungs of C57BL/6 mice (resistant to inhalational B. anthracis infection) had significantly higher levels of CXCL9, CXCL10, and CXCL11 than did the lungs of A/J mice (highly susceptible to infection). Increased CXC chemokine levels were associated with significantly reduced levels of spore germination within the lungs as determined by in vivo imaging. Taken together, our data demonstrate a novel antimicrobial role for host chemokines against B. anthracis that provides unique insight into host defense against inhalational anthrax; these data also support the notion for an innovative approach in treating B. anthracis infection as well as infections caused by other spore-forming organisms.

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