Determination of locally accurate solution protein structures and unambiguous stereo-specific 1H NMR assignments

Proteins ◽  
1991 ◽  
pp. 62-67 ◽  
Author(s):  
S. A. Sherman ◽  
Michael E. Johnson
Keyword(s):  
1H Nmr ◽  
Nmr Assignments ◽  
Protein Structures ◽  
Accurate Solution

scholarly journals Sequence-specific 1H-NMR assignments and determination of the secondary structure in aqueous solution of the cardiotoxins CTXIIa and CTXIIb from Naja mossambica mossambica

1987 ◽  
Vol 168 (3) ◽  
pp. 609-620 ◽  
Author(s):  
Gottfried OTTING ◽  
Wayne E. STEINMETZ ◽  
Pierre E. BOUGIS ◽  
Herve ROCHAT ◽  
Kurt WUTHRICH
Keyword(s):  
Aqueous Solution ◽  
Secondary Structure ◽  
1H Nmr ◽  
Nmr Assignments

Sequence-Specific 1H NMR Assignments and Determination of the Secondary Structure for the Activation Domain Isolated from Pancreatic Procarboxypeptidase B

Biochemistry ◽  
1990 ◽  
Vol 29 (32) ◽  
pp. 7515-7522 ◽  
Author(s):  
Josep Vendrell ◽  
Gerhard Wider ◽  
Francesc Aviles ◽  
Kurt Wuthrich
Keyword(s):  
Secondary Structure ◽  
1H Nmr ◽  
Nmr Assignments ◽  
Activation Domain

Is it necessary to calculate Young’s modulus in AFM nanoindentation experiments regarding biological samples?

2020 ◽  
Vol 12 ◽  
Author(s):  
S.V. Kontomaris ◽  
A. Malamou ◽  
A. Stylianou
Keyword(s):  
Young’S Modulus ◽  
Biological Samples ◽  
Young's Modulus ◽  
Reference Sample ◽  
Nonlinear Behavior ◽  
Accurate Method ◽  
Accurate Solution ◽  
Hertz Model ◽  
Work Done

Background: The determination of the mechanical properties of biological samples using Atomic Force Microscopy (AFM) at the nanoscale is usually performed using basic models arising from the contact mechanics theory. In particular, the Hertz model is the most frequently used theoretical tool for data processing. However, the Hertz model requires several assumptions such as homogeneous and isotropic samples and indenters with perfectly spherical or conical shapes. As it is widely known, none of these requirements are 100 % fulfilled for the case of indentation experiments at the nanoscale. As a result, significant errors arise in the Young’s modulus calculation. At the same time, an analytical model that could account complexities of soft biomaterials, such as nonlinear behavior, anisotropy, and heterogeneity, may be far-reaching. In addition, this hypothetical model would be ‘too difficult’ to be applied in real clinical activities since it would require very heavy workload and highly specialized personnel. Objective: In this paper a simple solution is provided to the aforementioned dead-end. A new approach is introduced in order to provide a simple and accurate method for the mechanical characterization at the nanoscale. Method: The ratio of the work done by the indenter on the sample of interest to the work done by the indenter on a reference sample is introduced as a new physical quantity that does not require homogeneous, isotropic samples or perfect indenters. Results: The proposed approach, not only provides an accurate solution from a physical perspective but also a simpler solution which does not require activities such as the determination of the cantilever’s spring constant and the dimensions of the AFM tip. Conclusion: The proposed, by this opinion paper, solution aims to provide a significant opportunity to overcome the existing limitations provided by Hertzian mechanics and apply AFM techniques in real clinical activities.

1H-NMR investigations. Application of trichloroacetyl isocyanate to structure determination of 3-, 6-, 7-, 7a- and 17-hydroxy steroids

1978 ◽  
Vol 43 (10) ◽  
pp. 2609-2618 ◽  
Author(s):  
Bruno Schönecker ◽  
Dieter Tresselt ◽  
Gerd Schubert ◽  
Ladislav Kohout ◽  
Jan Fajkoš
Keyword(s):  
1H Nmr ◽  
Structure Determination ◽  
Trichloroacetyl Isocyanate
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