Induction and Characterization of the Hypersensitive Reaction in Arabidopsis Thaliana L. in Response to a Pathogen Xanthomonas Campestris pv. Campestris and a Chemical Elicitor Lead Nitrate

A simple, quick and easy protocol was standardized for extraction of total DNA of the bacteria Xanthomonas axonopodis pv. phaseoli. The DNA obtained by this method had high quality and the quantity was enough for the Random Amplified Polymorphic DNA (RAPD) reactions with random primers, and Polymerase Chain Reaction (PCR) with primers of the hypersensitivity and pathogenicity gene (hrp). The DNA obtained was free of contamination by proteins or carbohydrates. The ratio 260nm/380nm of the DNA extracted ranged from 1.7 to 1.8. The hrp gene cluster is required by bacterial plant pathogen to produce symptoms on susceptible hosts and hypersensitive reaction on resistant hosts. This gene has been found in different bacteria as well as in Xanthomonas campestris pv. vesicatoria (9). The primers RST21 and RST22 (9) were used to amplify the hrp gene of nine different isolates of Xanthomonas axonopodis pv. phaseoli from Botucatu, São Paulo State, Brazil, and one isolate, "Davis". PCR amplified products were obtained in all isolates pathogenic to beans.

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Loss of chloroplast-localized protein phosphatase 2Cs in Arabidopsis thaliana leads to enhancement of plant immunity and resistance to Xanthomonas campestris pv. campestris infection

Molecular Plant Pathology ◽

10.1111/mpp.12596 ◽

2017 ◽

Vol 19 (5) ◽

pp. 1184-1195 ◽

Cited By ~ 11

Author(s):

Chiharu Akimoto-Tomiyama ◽

Shigeru Tanabe ◽

Hideyuki Kajiwara ◽

Eiichi Minami ◽

Hirokazu Ochiai

Keyword(s):

Arabidopsis Thaliana ◽

Protein Phosphatase ◽

Xanthomonas Campestris ◽

Plant Immunity ◽

Xanthomonas Campestris Pv Campestris

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Characterization of Xanthomonas campestris pv. campestris in Algeria

Phytopathologia Mediterranea ◽

10.36253/phyto-11726 ◽

2021 ◽

Vol 60 (1) ◽

pp. 51-62

Author(s):

Samia LAALA ◽

Sophie CESBRON ◽

Mohamed KERKOUD ◽

Franco VALENTINI ◽

Zouaoui BOUZNAD ◽

...

Keyword(s):

Xanthomonas Campestris ◽

Housekeeping Genes ◽

First Record ◽

Black Rot ◽

Physiological And Biochemical Characteristics ◽

Main Production ◽

Production Areas ◽

Cruciferous Plants ◽

Xanthomonas Campestris Pv Campestris

Xanthomonas campestris pv. campestris (Xcc) causes the black rot of cruciferous plants. This seed-borne bacterium is considered as the most destructive disease to cruciferous crops. Although sources of contamination are various, seeds are the main source of transmission. Typical symptoms of black rot were first observed in 2011 on cabbage and cauliflower fields in the main production areas of Algeria. Leaf samples displaying typical symptoms were collected during 2011 to 2014, and 170 strains were isolated from 45 commercial fields. Xcc isolates were very homogeneous in morphological, physiological and biochemical characteristics similar to reference strains, and gave positive pathogenicity and molecular test results (multiplex PCR with specific primers). This is the first record of Xcc in Algeria. Genetic diversity within the isolates was assessed in comparison with strains isolated elsewhere. A multilocus sequence analysis based on two housekeeping genes (gyrB and rpoD) was carried out on 77 strains representative isolates. The isolates grouped into 20 haplotypes defined with 68 polymorphic sites. The phylogenetic tree obtained showed that Xcc is in two groups, and all Algerian strains clustered in group 1 in three subgroups. No relationships were detected between haplotypes and the origins of the seed lots, the varieties of host cabbage, the years of isolation and agroclimatic regions.

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Genome-Scale Mutagenesis and Phenotypic Characterization of Two-Component Signal Transduction Systems in Xanthomonas campestris pv. campestris ATCC 33913

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-21-8-1128 ◽

2008 ◽

Vol 21 (8) ◽

pp. 1128-1138 ◽

Cited By ~ 45

Author(s):

Wei Qian ◽

Zhong-Ji Han ◽

Jun Tao ◽

Chaozu He

Keyword(s):

Signal Transduction ◽

Xanthomonas Campestris ◽

Sodium Dodecyl ◽

Phenotypic Characterization ◽

Phytopathogenic Bacterium ◽

Two Component ◽

Two Component Signal Transduction ◽

Xanthomonas Campestris Pv Campestris ◽

Signal Transduction Systems

The gram-negative bacterium Xanthomonas campestris pv. campestris is the causal agent of black rot disease of cruciferous plants. Its genome encodes a large repertoire of two-component signal transduction systems (TCSTSs), which consist of histidine kinases and response regulators (RR) to monitor and respond to environmental stimuli. To investigate the biological functions of these TCSTS genes, we aimed to inactivate all 54 RR genes in X. campestris pv. campestris ATCC 33913, and successfully generated 51 viable mutants using the insertion inactivation method. Plant inoculation identified two novel response regulator genes (XCC1958 and XCC3107) that are involved in virulence of this strain. Genetic complementation demonstrated that XCC3107, designated as vgrR (virulence and growth regulator), also affects bacterial growth and activity of extracellular proteases. In addition, we assessed the survival of these mutants under various stresses, including osmotic stress, high sodium concentration, heat shock, and sodium dodecyl sulfate exposure, and identified a number of genes that may be involved in the general stress response of X. campestris pv. campestris. Mutagenesis and phenotypic characterization of RR genes in this study will facilitate future studies on signaling networks in this important phytopathogenic bacterium.

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Characterization of genes encoding proteins containing HD-related output domain in Xanthomonas campestris pv. campestris

Antonie van Leeuwenhoek ◽

10.1007/s10482-016-0656-y ◽

2016 ◽

Vol 109 (4) ◽

pp. 509-522 ◽

Cited By ~ 2

Author(s):

Hsien-Ming Lee ◽

Chao-Tsai Liao ◽

Ying-Chuan Chiang ◽

Yu-Yin Chang ◽

Yu-Tzu Yeh ◽

...

Keyword(s):

Xanthomonas Campestris ◽

Genes Encoding ◽

Xanthomonas Campestris Pv Campestris ◽

Output Domain

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Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli

Archives of Biological Sciences ◽

10.2298/abs1302585p ◽

2013 ◽

Vol 65 (2) ◽

pp. 585-593 ◽

Cited By ~ 4

Author(s):

Tatjana Popovic ◽

Dragana Josic ◽

Mira Starovic ◽

P. Milovanovic ◽

N. Dolovac ◽

...

Keyword(s):

16S Rdna ◽

Xanthomonas Campestris ◽

Reference Strain ◽

Tween 80 ◽

Genotypic Characterization ◽

Hypodermic Syringe ◽

Xanthomonas Campestris Pv Campestris ◽

Reference Strains ◽

Phenotypic And Genotypic Characterization

Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35?C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.

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