Quantitative Traits and Genetic Markers: Analysis of a Factorial Mating Design in Larch

Abstract With random amplified polymorphic DNA (RAPD) markers, we have tagged a genomic region in Populus sp. involved in qualitative resistance to Melumpsora larici-populina. Our approach was based on three steps: use of RAPD markers that can be quickly and efficiently researched; application of “bulked segregant analysis” technique on individuals of one interspecific family P. trichocarpa × P. deltoides to search for RAPD markers linked to resistance; and validation of these markers in two other families linked with the first one in a 2 × 2 factorial mating design. Of five detected markers, only one marker M03/04_480 was polymorphic in the three segregating families, involving 89 individuals and four different parents. We have estimated the recombination value of 1 cM with 1 cM sampling error.

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Variation and Heritability Estimates of Anthocyanins and Their Relationship to Antioxidant Activity in a Red Raspberry Factorial Mating Design

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.130.4.534 ◽

2005 ◽

Vol 130 (4) ◽

pp. 534-542 ◽

Cited By ~ 17

Author(s):

Ann Marie Connor ◽

Tony K. McGhie ◽

M. Joseph Stephens ◽

Harvey K. Hall ◽

Peter A. Alspach

Keyword(s):

Antioxidant Activity ◽

Total Variation ◽

Total Variance ◽

Narrow Sense ◽

Red Raspberry ◽

Narrow Sense Heritability ◽

Mating Design ◽

Factorial Mating ◽

Heritability Estimates ◽

Factorial Mating Design

We determined variance components and narrow-sense heritability estimates for total and individual anthocyanin (ACY) content and antioxidant activity (AA) in fruit from 411 genotypes in a red raspberry (Rubus idaeus L.) factorial mating design based on 42 full-sib families derived from seven female and six male parents, harvested in 2002 and 2003. Within half-sib family total ACY content ranged from ≈1-60+ mg/100 g fruit in both seasons. The four major ACYs quantified by high-performance liquid chromatography also showed wide ranges each year. Female and male parent contributions to variation in total and individual ACYs were significant (P ≤ 0.001) in combined year analysis, and together accounted for 29% to 48% of the total variation. A substantial proportion of the female contribution was attributed to the use of a pigment-deficient R. parvifolius L. × R. idaeus hybrid derivative as a female parent. Female × male interaction was nonsignificant and contributed negligibly to total variance. Year effects accounted for <2.5% of variation in ACYs and were only marginally significant. Year interactions were negligible. Within family variation (among plots and within plot) accounted for ≈50% of the variation in total ACY and 62% to 69% of the variation in individual ACYs. Combined year narrow-sense heritability estimates were high (h2 = 0.54-0.90 for individual ACYs, 1.00 for total ACY) among all factorial genotypes, but moderate when the progeny of the R. parvifolius derivative were excluded (h2 = 0.45-0.78 for individual ACYs, 0.74 for total ACY). The latter estimates are applicable to breeding programs in which pigment-deficient genotypes are rarely or never used in breeding. Parental main effects were significant for AA, together accounting for 19% of total variance; female × male interaction was nonsignificant. Year effects were marginally significant and year interactions nonsignificant; together these sources of variation contributed <2% of total variation in AA. The majority of AA variation was found within- and among-plots within family. The phenotypic correlation between AA and total ACY was r = 0.53, and ranged from r = 0.21-0.46 between AA and individual ACYs; genetic correlations between AA and the ACYs were similar to the phenotypic correlations, suggesting predominantly additive genetic effects accounted for the phenotypic correlations. Linear modelling for AA based on individual ACYs and their interactions explained ≈0.53 of AA variation, substantially less than that explained by total phenolic content (R2 = 0.88). Our results show substantial variation and moderate to high narrow-sense heritability estimates for red raspberry ACYs, but ACY content and profile information are ineffective proxies and predictors for AA in red raspberry fruit.

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Accuracy of BLUP breeding values in a factorial mating design with mixed families and marker-based parentage assignment in rainbow trout Oncorhynchus mykiss

Aquaculture ◽

10.1016/j.aquaculture.2018.03.003 ◽

2018 ◽

Vol 490 ◽

pp. 350-354 ◽

Cited By ~ 2

Author(s):

Pierrick Haffray ◽

Florian Enez ◽

Jérôme Bugeon ◽

Hervé Chapuis ◽

Mathilde Dupont-Nivet ◽

...

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Parentage Assignment ◽

Breeding Values ◽

Mating Design ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Factorial Mating ◽

Factorial Mating Design ◽

Mixed Families

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Combining Ability of Husk Extension, Maysin Content, and Corn Earworm Resistance

Journal of the American Society for Horticultural Science ◽

10.21273/jashs04974-20 ◽

2021 ◽

Vol 146 (1) ◽

pp. 14-23

Author(s):

Virginia M. Moore ◽

William F. Tracy

Keyword(s):

Zea Mays ◽

Combining Ability ◽

Helicoverpa Zea ◽

Sweet Corn ◽

Corn Earworm ◽

Management Options ◽

Mating Design ◽

Cultivar Development ◽

Factorial Mating ◽

Factorial Mating Design

Corn earworm (Helicoverpa zea) is a destructive pest with limited management options in sweet corn (Zea mays) production. Increased husk extension and the presence of the C-glycosyl flavone maysin are two proposed mechanisms for improving corn earworm resistance in corn cultivars. A factorial mating design was conducted to test hybrid combinations of sweet corn inbreds with long husks and/or maysin to identify candidates for future cultivar development. The mating design had seven male parents, including three commercial sweet corn inbreds (Wh9261, We11401, and Wt1001) and four inbreds selected for maysin content (Maysin1, 2, 3, and 4), and five female parents, including two commercial sweet corn inbreds (Ia453su and Ia5125su) and three inbreds with long, thick, tight husks (A684su, A685su, and A686su). Hybrids were evaluated for ear length, husk length, maysin content, and corn earworm resistance at six environments in 2016 and 2017. Relationships between husk extension, maysin, and corn earworm resistance were inconsistent, but five inbreds produced hybrids with significantly lower corn earworm infestation and/or damage, demonstrating potential to confer resistance to the corn earworm.

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