Abstract
Background：Porcine circovirus type 3 (PCV3) is a newly detected pathogen from pigs in the past few years. As the infection rate of PCV3 in the herd is getting higher and wider, the infection is more and more serious. The virus with unclear clinical symptoms and pathogenesis is also paid more and more attention. And detection of pathogenic antibodies has become an important issue. Methods:In this experiment, Loop-mediated isothermal amplification (LAMP) was combined with Lateral flow dipstick (LFD) to establish a rapid and convenient detection method (LAMP-LFD). Results:The results showed that the band was darkened at a nucleic acid concentration of 0.2 pg/ μL using a conventional PCR method, and a clear detection line was observed at a nucleic acid concentration of 0.2 fg/μL using the LAMP-LFD method. The primer and hybridization probe are only compatible with PCV3.Compared to the PCR method, the LAMP-LFD method has a shorter time (about 1 h) and requires less instrumentation. Conclusions: The test results show that the LAMP-LFD method has extremely high sensitivity and specificity. The operation is simple and quick. The visual effect of the detection results is better than that of PCR and single LAMP test, which can bring convenience in practical production applications.
CHANGES IN NUCLEIC ACID CONCENTRATION DURING THE DEVELOPMENT OF THE CHICK EMBRYO