Structural and Functional Analysis of the FAS1 Gene Encoding Fatty Acid Synthase β-Subunit in Methylotrophic Yeast Hansenula Polymorpha

2003 ◽  
pp. 61-64 ◽  
Author(s):  
Y. Kaneko ◽  
K. Rueksomtawin ◽  
T. Maekawa ◽  
S. Harashima
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Β Subunit ◽  
Gene Encoding

Functional analysis of very long-chain fatty acid elongase gene, HpELO2, in the methylotrophic yeast Hansenula polymorpha

2007 ◽  
Vol 76 (2) ◽  
pp. 417-427 ◽  
Author(s):  
Phatthanon Prasitchoke ◽  
Yoshinobu Kaneko ◽  
Minetaka Sugiyama ◽  
Takeshi Bamba ◽  
Eiichiro Fukusaki ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Fatty Acid Elongase ◽  
Long Chain

scholarly journals A Fatty Acid Synthase Gene in Cochliobolus carbonum Required for Production of HC-Toxin, Cyclo(d-Prolyl-l-Alanyl- d-Alanyl- l-2-Amino-9,10-Epoxi-8-Oxodecanoyl)

1997 ◽  
Vol 10 (2) ◽  
pp. 207-214 ◽  
Author(s):  
Joong-Hoon Ahn ◽  
Jonathan D. Walton
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Cyclic Peptide ◽  
Decanoic Acid ◽  
Toxin Production ◽  
Gene Encoding ◽  
Cochliobolus Carbonum ◽  
Primary Amino ◽  
Hc Toxin

The fungal maize pathogen Cochliobolus carbonum produces a phytotoxic and cytostatic cyclic peptide, HC-toxin, of structure cyclo(D-prolyl-L-alanyl-D-alanyl-L-Aeo), in which Aeo stands for 2-amino-9,10-epoxi-8-oxodecanoic acid. Here we report the isolation of a gene, TOXC, that is present only in HC-toxin-producing (Tox2+) fungal strains. TOXC is present in most Tox2+ strains in three functional copies, all of which are on the same chromosome as the gene encoding HC-toxin synthetase. When all copies of TOXC are mutated by targeted gene disruption, the fungus grows and sporulates normally in vitro but no longer makes HC-toxin and is not pathogenic, indicating that TOXC has a specific role in HC-toxin production and hence virulence. The TOXC mRNA is 6.5 kb and the predicted product has 2,080 amino acids and a molecular weight of 233,000. The primary amino acid sequence is highly similar (45 to 47% identity) to the β subunit of fatty acid synthase from several lower eukaryotes, and contains, in the same order as in other β subunits, domains predicted to encode acetyl transferase, enoyl reductase, dehydratase, and malonyl-palmityl transferase. The most plausible function of TOXC is to contribute to the synthesis of the decanoic acid backbone of Aeo.

scholarly journals Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus

Microbiology ◽  
2012 ◽  
Vol 158 (4) ◽  
pp. 1037-1044 ◽  
Author(s):  
Leyre Palacios-Chaves ◽  
Amaia Zúñiga-Ripa ◽  
Ana Gutiérrez ◽  
Yolanda Gil-Ramírez ◽  
Raquel Conde-Álvarez ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Brucella Abortus ◽  
Fatty Acid Synthase ◽  
Cyclopropane Fatty Acid

Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha

Gene ◽  
2014 ◽  
Vol 533 (1) ◽  
pp. 110-118 ◽  
Author(s):  
Juthaporn Sangwallek ◽  
Yoshinobu Kaneko ◽  
Tomoya Tsukamoto ◽  
Makoto Marui ◽  
Minetaka Sugiyama ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Hansenula Polymorpha ◽  
Fatty Acid Desaturases ◽  
Genes Encoding
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