Single-Cell Transcriptome Sequencing Using Microfluidics

2020 ◽  
pp. 1-25
Author(s):  
Yu-Chih Chen ◽  
Seungwon Jung ◽  
Yehyun Choi ◽  
Euisik Yoon
Keyword(s):  
Single Cell ◽  
Transcriptome Sequencing ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single-cell transcriptome sequencing analysis reveals gynaecomastia to breast cancer transition

The Breast ◽  
2021 ◽  
Vol 56 ◽  
pp. S26
Author(s):  
H. Sun ◽  
C. Yang ◽  
Z. Wang ◽  
L. Shi ◽  
T. Xia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Single Cell ◽  
Transcriptome Sequencing ◽  
Sequencing Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

Single-Cell Transcriptome Sequencing Using Microfluidics

2021 ◽  
pp. 607-630
Author(s):  
Yu-Chih Chen ◽  
Seungwon Jung ◽  
Yehyun Choi ◽  
Euisik Yoon
Keyword(s):  
Single Cell ◽  
Transcriptome Sequencing ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single Cell Transcriptome Sequencing Reveals the Potential Mechanism of Heterogeneity in Immunoregulatory Function Between Mesenchymal Stromal Cells

2021 ◽  
Author(s):  
Siyu Cai ◽  
Chuiqin Fan ◽  
Lichun Xie ◽  
Huifeng Zhong ◽  
Aijia Li ◽  
...  
Keyword(s):  
Single Cell ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Transcriptome Sequencing ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Umbilical Cord ◽  
Tnf Α ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome ◽  
Immunomodulatory Function

Abstract Background: Mesenchymal stromal cells (MSCs) could be applied for the treatment of immune-related diseases. However, some studies have found there is enormous heterogeneity in immunomodulatory function of MSCs isolated from different tissue. At present, the underlying mechanism of heterogeneity in immunoregulatory function is still unclear. Methods: In this study, the foreskin mesenchymal stromal cells (FSMSCs) and human umbilical cord mesenchymal stromal cells (HuMSCs) were isolated and cultured to the 3rd passage. Cell types were confirmed according to the standard of International Association for Cell Therapy. Then, FSMSCs and HuMSCs were co-cultured with human peripheral blood mononuclear cells (PBMC) stimulated by lipopolysaccharides (LPS) in viro respectively. And the supernatant was sampled for enzyme-linked immunosorbent assay to investigate the secretion of IL-1β, IL-6, IL-10, TNF-α and TGF-β. Finally, single cell transcriptome sequencing was performed in order to elucidate the mechanism for the difference of immunomodulatory function.Results: FSMSCs and HuMSCs are successfully identified as MSCs. When co-cultured with LPS pre-treated PBMC, FSMSCs and HuMSCs could effectively reduce the secretion of IL-1β and TNF-α. But FSMSCs were able to stimulate the PBMC to secrete more IL-10, TGF-β and IL-6. Furthermore, 4 MSCs subsets in integrated data were identified, including Proliferative MSCs , Pericyte, Immune MSCs and Progenitor Proliferative MSCs. Among them, the proportions of Immune MSCs in FSMSCs and HUMSCs were 56% and 10% respectively. Varieties of immune-related genes, gene sets and regulons were enriched in Immune MSCs. And Immune MSCs with powful transcriptional activity were found to be near to Pericyte at the degree of differentiation and closed to other cell subsets. Finally, the foreskin tissue might be an ideal source of isolating Immune MSCs when comparing the subset composition of MSCs derived from adipose tissue and bone marrow from public database. Conclusions: Immune MSCs may play a key role in the heterogeneity of immunoregulatory function. It is a new insight that Immune MSCs could be isolated and better applied for the treatment of immune-related diseases without being limited by the heterogeneity of immunomodulatory function derived from different tissues.

scholarly journals Exploiting antigen receptor information to quantify index switching in single-cell transcriptome sequencing experiments

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208484 ◽  
Author(s):  
Ying Yao ◽  
Asima Zia ◽  
Łukasz Wyrożemski ◽  
Ida Lindeman ◽  
Geir Kjetil Sandve ◽  
...  
Keyword(s):  
Single Cell ◽  
Transcriptome Sequencing ◽  
Antigen Receptor ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome
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