Molecular Approaches for Harvesting Natural Diversity for Crop Improvement

Author(s):  
Pawan Saini ◽  
Pooja Saini ◽  
Jashan Jot Kaur ◽  
Rose Mary Francies ◽  
Mudasir Gani ◽  
...  
2012 ◽  
Vol 4 (4) ◽  
pp. 115-119 ◽  
Author(s):  
Allah BAKHSH ◽  
Abdul Qayyum RAO ◽  
Ahmad Ali SHAHID ◽  
Tayyab HUSNAIN

The production of transgenic plants with stable, high-level transgene expression is important for the success of crop improvement programs based on genetic engineering. The present study was conducted to evaluate genomic integration and spatio temporal expression of an insecticidal gene (cry2A) in pre-existing transgenic lines of cotton. Genomic integration of cry2A was evaluated using various molecular approaches. The expression levels of cry2A were determined at vegetative and reproductive stages of cotton at regular intervals. These lines showed a stable integration of insecticidal gene in advance lines of transgenic cotton whereas gene expression was found variable with at various growth stages as well as in different plant parts throughout the season. The leaves of transgenic cotton were found to have maximum expression of cry2A gene followed by squares, bolls, anthers and petals. The protein level in fruiting part was less as compared to other parts showing inconsistency in gene expression. It was concluded that for culturing of transgenic crops, strategies should be developed to ensure the foreign genes expression efficient, consistent and in a predictable manner.


2021 ◽  
Vol 10 (4) ◽  
pp. 2784-2803

The application of nanotechnology in the field of agriculture completely changed the present farming practices. One of the emerging challenges in the current agricultural system is developing disease resistance and increased productivity without accumulating environmental pollutants. Most of the applied conventional chemical fertilizers and pesticides have several side effects on both plants and the environment. Nanotechnology-based crop protection strategies have the potential to induce disease tolerance and enhancement of crop yield. Benefits of using nanoparticles in the agricultural fields include reducing environmental pollutants, rapid disease diagnosis, simple preparation process, less toxicity, and cost-effectiveness. Green synthesis of nanoparticles using plant extracts gained special attention as it is reproducible, biodegradable, and very effective against several plant pathogenic microbes. In the present review, we have discussed how different nanoparticles are being used to induce disease tolerance in crops, different types of nanoparticles, synthesis and characterization, uptake, and translocation process in plants. The molecular approaches of disease management using nanoparticles and the application of nanobiosensors are also enlightened in this review.


Author(s):  
R. B. Austin ◽  
R. B. Flavell ◽  
I. E. Henson ◽  
H. J. B. Lowe

Sign in / Sign up

Export Citation Format

Share Document