Nucleotide sequence analysis of a cDNA clone encoding malate synthase of castor bean (Ricinus communis) reveals homology to DAL7, a gene involved in allantoin degradation in Saccharomyces cerevisiae

1990 ◽  
Vol 15 (3) ◽  
pp. 501-504 ◽  
Author(s):  
Dolores Rodriguez ◽  
Rebecca S. Ginger ◽  
Alison Baker ◽  
Don H. Northcote
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Malate Synthase ◽  
Nucleotide Sequence Analysis

scholarly journals Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase.

1991 ◽  
Vol 11 (1) ◽  
pp. 370-380 ◽  
Author(s):  
K I Minard ◽  
L McAlister-Henn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Carbon Source ◽  
Malate Dehydrogenase ◽  
Sodium Dodecyl ◽  
Yeast Cells ◽  
Nucleotide Sequence Analysis ◽  
Haploid Strain

The major nonmitochondrial isozyme of malate dehydrogenase (MDH2) in Saccharomyces cerevisiae cells grown with acetate as a carbon source was purified and shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a subunit molecular weight of approximately 42,000. Enzyme assays and an antiserum prepared against the purified protein were used to screen a collection of acetate-nonutilizing (acetate-) yeast mutants, resulting in identification of mutants in one complementation group that lack active or immunoreactive MDH2. Transformation and complementation of the acetate- growth phenotype was used to isolate a plasmid carrying the MDH2 gene from a yeast genomic DNA library. The amino acid sequence derived from complete nucleotide sequence analysis of the isolated gene was found to be extremely similar (49% residue identity) to that of yeast mitochondrial malate dehydrogenase (molecular weight, 33,500) despite the difference in sizes of the two proteins. Disruption of the MDH2 gene in a haploid yeast strain produced a mutant unable to grow on minimal medium with acetate or ethanol as a carbon source. Disruption of the MDH2 gene in a haploid strain also containing a disruption in the chromosomal MDH1 gene encoding the mitochondrial isozyme produced a strain unable to grow with acetate but capable of growth on rich medium with glycerol as a carbon source. The detection of residual malate dehydrogenase activity in the latter strain confirmed the existence of at least three isozymes in yeast cells.

Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase

1991 ◽  
Vol 11 (1) ◽  
pp. 370-380
Author(s):  
K I Minard ◽  
L McAlister-Henn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Carbon Source ◽  
Malate Dehydrogenase ◽  
Sodium Dodecyl ◽  
Yeast Cells ◽  
Nucleotide Sequence Analysis ◽  
Haploid Strain

The major nonmitochondrial isozyme of malate dehydrogenase (MDH2) in Saccharomyces cerevisiae cells grown with acetate as a carbon source was purified and shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a subunit molecular weight of approximately 42,000. Enzyme assays and an antiserum prepared against the purified protein were used to screen a collection of acetate-nonutilizing (acetate-) yeast mutants, resulting in identification of mutants in one complementation group that lack active or immunoreactive MDH2. Transformation and complementation of the acetate- growth phenotype was used to isolate a plasmid carrying the MDH2 gene from a yeast genomic DNA library. The amino acid sequence derived from complete nucleotide sequence analysis of the isolated gene was found to be extremely similar (49% residue identity) to that of yeast mitochondrial malate dehydrogenase (molecular weight, 33,500) despite the difference in sizes of the two proteins. Disruption of the MDH2 gene in a haploid yeast strain produced a mutant unable to grow on minimal medium with acetate or ethanol as a carbon source. Disruption of the MDH2 gene in a haploid strain also containing a disruption in the chromosomal MDH1 gene encoding the mitochondrial isozyme produced a strain unable to grow with acetate but capable of growth on rich medium with glycerol as a carbon source. The detection of residual malate dehydrogenase activity in the latter strain confirmed the existence of at least three isozymes in yeast cells.

Cloning of metallothionein cDNA from neonatal rat liver

1982 ◽  
Vol 2 (10) ◽  
pp. 761-768 ◽  
Author(s):  
J. F. B. Mercer ◽  
P. Hudson
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Rat Liver ◽  
Cdna Clone ◽  
Untranslated Region ◽  
Neonatal Rat ◽  
Nucleotide Sequence Analysis ◽  
Coding Sequence ◽  
Rat Livers ◽  
I Gene

A metallothionein cDNA clone was isolated from a cDNA bank prepared from neonatal rat liver poly(A)-containing RNA by a colony screening procedure using [32P]cDNA probes prepared from mRNA of either metal-induced or uninduced rat livers. Nucleotide sequence analysis of this clone showed that it contained the entire 3′ untranslated region and 30% of the coding sequence for a rat metallothionein. The sequence is remarkably homologous with the mouse metallothionein-I gene.

Nucleotide sequence analysis of the BALB/c murine sarcoma virus transforming gene.

1985 ◽  
Vol 53 (3) ◽  
pp. 984-987 ◽  
Author(s):  
E P Reddy ◽  
D Lipman ◽  
P R Andersen ◽  
S R Tronick ◽  
S A Aaronson
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
Transforming Gene
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