Bioreactor studies of the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis

1994 ◽  
Vol 36 (2) ◽  
pp. 205-209 ◽  
Author(s):  
V�ronique Jay ◽  
Simone Genestier ◽  
Jean-Claude Courduroux
1989 ◽  
Vol 90 (3) ◽  
pp. 921-927 ◽  
Author(s):  
John C. Thomas ◽  
Craig Nessler ◽  
Frank Katterman

This paper deals with problems of somatic embryogenesis (totipotency) of carrot cells in liquid culture and when dispersed in thin films of nutrient agar. The critical events that intervene between somatic cells and embryo plantlets during morphogenesis are delineated. Factors that affect the course of these events are described with special reference to the stimuli which permit the very smallest units to develop into organized structures. Special attention is directed to the importance of a protracted period of darkness and the use of an ‘embryo-conditioned medium’ as interacting stimuli that facilitate the early stages of development. Means of arresting and releasing the development of pro-embryonic units are discussed. Aspects of growth and form of the developing cultures are presented photographically at different levels. The behaviour of free protoplasts from pro-embryonic units is described and contrasted with that of intact totipotent cells. The significance of all these observations is examined in the light of practical applications and in relation to problems of development.


1998 ◽  
Vol 15 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Yoshinobu TACHIKAWA ◽  
Tsutomu SAITOU ◽  
Hiroshi KAMADA ◽  
Hiroshi HARADA

Planta ◽  
1995 ◽  
Vol 197 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Marc Kreuger ◽  
Gerrit-Jan van Holst

1991 ◽  
Vol 9 (12) ◽  
pp. 655-658 ◽  
Author(s):  
Hitoshi Wake ◽  
Hironori Umetsu ◽  
Yoshihiro Ozeki ◽  
Koichiro Shimomura ◽  
Tadashi Matsunaga

Biologia ◽  
2019 ◽  
Vol 74 (6) ◽  
pp. 695-707 ◽  
Author(s):  
Sathish Sundararajan ◽  
Safia Nayeem ◽  
Sivakumar Subiramani ◽  
Venkatesh Rajendran ◽  
Sathishkumar Ramalingam

Planta ◽  
1997 ◽  
Vol 203 (2) ◽  
pp. 188-195 ◽  
Author(s):  
Marcel A. J. Toonen ◽  
Ed D. L. Schmidt ◽  
Ab van Kammen ◽  
Sacco C. de Vries

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