Promotive and inhibitory effects of diverse arabinogalactan proteins on Daucus carota L. somatic embryogenesis

Planta ◽  
1997 ◽  
Vol 203 (2) ◽  
pp. 188-195 ◽  
Author(s):  
Marcel A. J. Toonen ◽  
Ed D. L. Schmidt ◽  
Ab van Kammen ◽  
Sacco C. de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Arabinogalactan Proteins ◽  
Inhibitory Effects ◽  
Daucus Carota L

Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L.

Planta ◽  
1993 ◽  
Vol 189 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Marc Kreuger ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Arabinogalactan Proteins ◽  
Daucus Carota L

scholarly journals Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced

2020 ◽  
Vol 21 (21) ◽  
pp. 8126
Author(s):  
Michał Kuczak ◽  
Ewa Kurczyńska
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Fate ◽  
Cell Walls ◽  
Arabinogalactan Proteins ◽  
Cell Wall Composition ◽  
Cell Reprogramming ◽  
Daucus Carota L ◽  
Negative Marker ◽  
Spatio Temporal ◽  
Wall Components

Changes in the composition of the cell walls are postulated to accompany changes in the cell’s fate. We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate. Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis. Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.

scholarly journals Interruption of Somatic Embryogenesis in Daucus carota L. by 5-Bromodeoxyuridine

1989 ◽  
Vol 90 (3) ◽  
pp. 921-927 ◽  
Author(s):  
John C. Thomas ◽  
Craig Nessler ◽  
Frank Katterman
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Daucus Carota L

Observations on growth and morphogenesis in cultured cells of carrot ( Daucus carota L.)

1975 ◽  
Vol 273 (922) ◽  
pp. 33-53 ◽  
Keyword(s):  
Thin Films ◽  
Somatic Embryogenesis ◽  
Special Reference ◽  
Daucus Carota ◽  
Liquid Culture ◽  
Cultured Cells ◽  
Daucus Carota L ◽  
Practical Applications ◽  
Carrot Cells ◽  
Different Levels

This paper deals with problems of somatic embryogenesis (totipotency) of carrot cells in liquid culture and when dispersed in thin films of nutrient agar. The critical events that intervene between somatic cells and embryo plantlets during morphogenesis are delineated. Factors that affect the course of these events are described with special reference to the stimuli which permit the very smallest units to develop into organized structures. Special attention is directed to the importance of a protracted period of darkness and the use of an ‘embryo-conditioned medium’ as interacting stimuli that facilitate the early stages of development. Means of arresting and releasing the development of pro-embryonic units are discussed. Aspects of growth and form of the developing cultures are presented photographically at different levels. The behaviour of free protoplasts from pro-embryonic units is described and contrasted with that of intact totipotent cells. The significance of all these observations is examined in the light of practical applications and in relation to problems of development.

Bioreactor studies of the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis

1994 ◽  
Vol 36 (2) ◽  
pp. 205-209 ◽  
Author(s):  
V�ronique Jay ◽  
Simone Genestier ◽  
Jean-Claude Courduroux
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Medium Ph ◽  
Daucus Carota L

scholarly journals Changes in Protein Pattern during Stress-induction of Carrot (Daucus carota L.) Somatic Embryogenesis.

1998 ◽  
Vol 15 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Yoshinobu TACHIKAWA ◽  
Tsutomu SAITOU ◽  
Hiroshi KAMADA ◽  
Hiroshi HARADA
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Protein Pattern ◽  
Stress Induction ◽  
Daucus Carota L

Arabinogalactan-protein epitopes in somatic embryogenesis of Daucus carota L.

Planta ◽  
1995 ◽  
Vol 197 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Marc Kreuger ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Arabinogalactan Protein ◽  
Daucus Carota L

Extracts of Marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L.

1991 ◽  
Vol 9 (12) ◽  
pp. 655-658 ◽  
Author(s):  
Hitoshi Wake ◽  
Hironori Umetsu ◽  
Yoshihiro Ozeki ◽  
Koichiro Shimomura ◽  
Tadashi Matsunaga
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Marine Cyanobacteria ◽  
Daucus Carota L
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