Somatic embryogenesis and plant regeneration in olive (Olea europaea L.)

1988 ◽  
Vol 14 (3) ◽  
pp. 207-214 ◽  
Author(s):  
E. Rugini
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Olea Europaea ◽  
Olea Europaea L ◽  
Olea Europaéa

Somatic embryogenesis from cultured zygotic explants of some olive cultivars (Olea europaea L.)

2014 ◽  
Vol 31 ◽  
pp. S152-S153
Author(s):  
Sara Oulbi ◽  
Ibrahim Toufik ◽  
Ilham Belkoura
Keyword(s):  
Somatic Embryogenesis ◽  
Olea Europaea ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olea Europaéa

scholarly journals Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 901
Author(s):  
Sara Oulbi ◽  
Kaoutar Kohaich ◽  
Mohammed Baaziz ◽  
Ilham Belkoura ◽  
Kenza Loutfi
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Olea Europaea ◽  
Biochemical Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Zeatin Riboside ◽  
Naphthaleneacetic Acid ◽  
Olea Europaea L ◽  
Highly Active ◽  
Olea Europaéa

As part of the search for biochemical markers of somatic embryogenesis in tissue cultures of olive (Olea europaea L.), peroxidases (POXs) in both the soluble and ionically wall-bound fractions were studied in two reputed olive cultivars (cvs.): “Picholine Marocaine” and “Dahbia”. In order to carry out embryogenesis induction, proximal cotyledons were cultured in modified olive medium (OMc) supplemented with 25 μM indole-3-butylic acid (IBA) and 2.5 μM 2-isopentenyladenine (2iP), while distal leaf fragments (somatic explants) were cultured in OMc supplemented with 4.56 µM zeatin riboside (ZR) and 10.25 µM 1-naphthaleneacetic acid (NAA). Regarding embryogenic potentials, the zygotic explants (cv. Picholine Marocaine: 43.39%; cv. Dahbia: 53.41%) were more regenerative than the somatic explants (cv. Picholine Marocaine: 13.05%; cv. Dahbia: 19.51%). The enzyme assay showed a higher POX activity in embryogenic calluses (ECs) than in nonembryogenic calluses (NECs) for the zygotic explants in both studied cultivars. When expressed as units per milligram of proteins (U mg−1 proteins), the highest total POXs activities (soluble POXs + ionically wall-bound POXs) were found in the ECs derived from the zygotic explants; for cv. Dahbia, 65% of the enzyme activities came from the ionically wall-bound fractions. Polyacrylamide gel electrophoresis showed that the ECs of the highly active cv. Dahbia were characterized by highly active isoperoxidases that were revealed in four migration zones, particularly a doublet in the A4 zone (Rf 0.70–0.73) present in the ionically wall-bound POXs. The fast-moving anodic POXs of the ionically wall-bound fractions could be adopted as an early electrophoretic test to determine the embryogenesis capacities in olive tissue culture materials. As biochemical markers, the POX enzyme and its profile in fractions, i.e., as soluble POXs and ionically wall-bound POXs, can offer a valuable tool for improving the tissue culture of olive via somatic embryogenesis.

Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) ‘Chetoui’

2010 ◽  
Vol 33 (2) ◽  
pp. 319-324 ◽  
Author(s):  
El Bahri Trabelsi ◽  
Selima Naija ◽  
Nedra Elloumi ◽  
Zina Belfeleh ◽  
Monji Msellem ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Suspension ◽  
Olea Europaea ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Olea Europaea L ◽  
Olea Europaéa
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