Assessment of allozyme variation among New Zealand populations of Gracilaria chilensis (Gracialiares, Rhodophyta) using starch-gel electrophoresis

Hydrobiologia ◽  
1993 ◽  
Vol 260-261 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Sompop Intasuwan ◽  
Margaret E. Gordon ◽  
Charles H. Daugherty ◽  
Graeme C. Lindsay
Keyword(s):  
New Zealand ◽  
Gel Electrophoresis ◽  
Allozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Gracilaria Chilensis ◽  
Starch Gel

Genetic diversity in red pine: evidence for low genic heterozygosity

1977 ◽  
Vol 7 (2) ◽  
pp. 343-347 ◽  
Author(s):  
D. P. Fowler ◽  
R. W. Morris
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Allozyme Variation ◽  
Red Pine ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Coniferous Species ◽  
Low Degree ◽  
Starch Gel ◽  
Genetically Determined

Starch gel electrophoresis was used to survey for genetically determined enzyme mobility differences among 297 megagametophytes of red pine (Pinusresinosa Ait.) from five widely separated geographical sources. Consistent and reproducible enzyme banding patterns were observed with five of the seven isozyme systems assayed. No variation in band mobility was observed in any of these systems. This result stands in contrast with those reported from surveys of allozyme variation in other coniferous species but is consistent with the low degree of genetic variation observed in red pine for higher levels of genetic organization. It is concluded that red pine is genetically depauperate.Possible explanations for restricted genetic diversity are discussed. The most plausible explanation suggests that red pine was at sometime, possibly during the Pleistocene, reduced to a small refugial population and has yet to reestablish equilibrium heterozygosity.

scholarly journals Isozymes for Cultivar Identification in Kiwifruit

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 899-902 ◽  
Author(s):  
R. Messina ◽  
R. Testolin ◽  
M. Morgante
Keyword(s):  
New Zealand ◽  
Genetic Markers ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Discriminating Power ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Simultaneous Comparison

The usefulness of isozyme banding patterns as genetic markers in kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] was investigated using starch gel electrophoresis. Fifty-four entries putatively belonging to seven female and two male kiwifruit cultivars were examined for 13 enzyme systems (AAT, ACO, GDH, G6PDH, IDH, MDH, ME, MNR, NDH, 6PGD, PGI, PGM, and SKDH). Four enzyme systems, ACO, MDH, NDH, and SKDH, showed identical banding patterns in all clones surveyed. Of the remaining enzymes, AAT, PGI, and PGM had the best discriminating power. Six enzyme systems (GDH, G6PDH, IDH, ME, MNR, and 6PGD), though showing polymorphic banding patterns, were poorly resolved. All the New Zealand cultivars were uniquely identified by the simultaneous comparison of the AAT, PGI, and PGM zymograms. Some enzyme systems were also polymorphic among plants within the same cultivar, thus proving the heterogeneity of kiwifruit material introduced into Europe in the early 1970s.

scholarly journals CHARACTERISTICS OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODY ISOLATED FROM HYPERIMMUNE RABBITS

1966 ◽  
Vol 123 (4) ◽  
pp. 599-614 ◽  
Author(s):  
C. Kirk Osterland ◽  
Edward J. Miller ◽  
Walter W. Karakawa ◽  
Richard M. Krause
Keyword(s):  
New Zealand ◽  
Specific Antibody ◽  
Gel Electrophoresis ◽  
Limited Range ◽  
Carbohydrate Antigen ◽  
Starch Gel Electrophoresis ◽  
Normal Complement ◽  
Zone Electrophoresis ◽  
Group A ◽  
Starch Gel

Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated γ-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of γ-globulin. The bulk of this γ-globulin, identified as γG-globulin, was homogeneous by zone electrophoresis and of specificity directed against the Group A-variant carbohydrate antigen. L chains isolated from specific antibody obtained from an immune precipitate were distributed as a single band on starch gel electrophoresis, whereas the normal γ-globulin traveled as a diffuse smear. These data suggest that the rabbit streptococcal Group A-variant antibodies possess a limited range of physicochemical properties and electrophoretic mobility compared to that generally observed for the normal complement of γ-globulin.

Associations between individual gliadin proteins and quality, agronomic and morphological attributes of wheat cultivars

1982 ◽  
Vol 33 (3) ◽  
pp. 409 ◽  
Author(s):  
CW Wrigley ◽  
PJ Robinson ◽  
WT Williams
Keyword(s):  
New Zealand ◽  
North America ◽  
Gel Electrophoresis ◽  
Spanning Tree ◽  
Minimum Spanning Tree ◽  
Starch Gel Electrophoresis ◽  
Wheat Cultivars ◽  
Morphological Attributes ◽  
Starch Gel ◽  
Gliadin Protein

The gliadin protein compositions of 79 wheat genotypes from Australia, New Zealand and North America, and one rye cultivar have been examined by starch gel electrophoresis. Interrelationships in the patterns thus obtained were investigated using a computer-generated inverse minimum spanning tree. Certain components were frequently found to occur together, presumably because their synthesis is controlled by closely linked genes. In addition, by using a network-generating program, associations between gliadins and many morphological (plant, head and grain), agronomic and quality attributes were studied. In many cases, specific gliadin components were found to be closely associated with certain attributes. In such cases a direct cause and effect cannot be inferred, but the approach indicates associations that warrant further study.

Genetic and morphological variation among populations of Oxyna parietina (Diptera: Tephritidae) across a European transect

1992 ◽  
Vol 70 (6) ◽  
pp. 1120-1128 ◽  
Author(s):  
Sabine Eber ◽  
Roland Brandl ◽  
Stefan Vidal
Keyword(s):  
Gene Flow ◽  
Morphological Variation ◽  
Gel Electrophoresis ◽  
Regional Scale ◽  
Allozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Allozyme Data ◽  
Starch Gel ◽  
Wing Morphometrics ◽  
Morphological Patterns

Genetic and morphological variation in the phytophagous tephritid Oxyna parietina (L.) was investigated across a transect through Central Europe by starch-gel electrophoresis to detect allozyme variation and by wing morphometrics. Very low allozyme differentiation was found on both a local and a regional scale, and is best explained by high rates and distances of gene flow. Nevertheless, there exists a smooth cline of allele frequencies from northern to southern populations. We tentatively interpret this cline as being a consequence of selection by environmental factors. The morphological patterns are not consistent with the allozyme data.

Allozyme variation within and among cultivated varieties of sweet chestnut (Castaneasativa)

1994 ◽  
Vol 24 (6) ◽  
pp. 1160-1165 ◽  
Author(s):  
S. Flneschi ◽  
M.E. Malvolti ◽  
M. Morgante ◽  
G.G. Vendramin
Keyword(s):  
Gel Electrophoresis ◽  
Southern Italy ◽  
Allozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Enzyme Gene ◽  
Sweet Chestnut ◽  
Long Time ◽  
Starch Gel ◽  
High Degree ◽  
Southern European Countries

Sweet chestnut (Castaneasativa Mill.) is a species that has been cultivated and propagated through grafting for a long time in Italy and Southern European countries. The genetic variability within and among different varieties was analyzed by means of starch gel electrophoresis. Twenty cultivated varieties originating from three different areas located in northern, central, and southern Italy, were analyzed at six polymorphic enzyme gene loci. Our results show a relatively high degree of homogeneity both among individuals of the same variety and among varieties of the same area; on the other hand, high values of genetic distance were found among different geographic areas. The agamic propagation method of this species may have caused a reduction of the genetic diversity within varieties. The causes and consequences of the loss of genetic variation in these varieties are discussed.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

Sign in / Sign up

Export Citation Format

Share Document