The use of flax (Linum usitatissimum) as a model system for studies on organogenesis in vitro: the effect of different carbohydrates

1992 ◽  
Vol 28 (2) ◽  
pp. 163-166 ◽  
Author(s):  
Stephen Millam ◽  
Diane Davidson ◽  
Wayne Powell
Keyword(s):  
Linum Usitatissimum ◽  
Model System ◽  
Organogenesis In Vitro

In vitro callusogenesis and organogenesis of different Linum usitatissimum L. accessions

2018 ◽  
pp. 49-58
Author(s):  
S.V. Mishchenko ◽  
L.M. Krivosheeva
Keyword(s):  
Czech Republic ◽  
Linum Usitatissimum ◽  
Cultivation Conditions ◽  
Genetic Origin ◽  
Linum Usitatissimum L ◽  
Murashige And Skoog Medium ◽  
Organogenesis In Vitro ◽  
Regeneration In Vitro ◽  
Number Of Shoots

Goal. The study of different genetic origin collection samples of flax (Linum usitatissimum L.) for the frequency and intensity of callusogenesis and organogenesis in vitro conditions, the establishment of differences in convar. elongatum, intermedia and humile was the goal of our research. Results and Discussion. The L. usitatissimum L. species is significantly capable of forming callus and shoot regeneration in vitro under cultivation conditions with a photoperiod of 16 hours, relative humidity of 60 – 80 %, air temperature of 22 – 24°С and agarized Murashige and Skoog medium, supplemented 0.05 mg/L of 1‑naphthylacetic acid, 1.0 mg/L of 6‑benzylaminopurine and 30 g/L of sucrose. The frequency and intensity of callusogenesis and organogenesis depends on the genotype. Minimum and maximum values of signs were set for Глінум (UF0401603), Кром (UF0401494), Орион (UF0401867), Есмань (UF0402071), Белита (UF0402134), Nor Man (UF0401792), Mapun M.A. (UF0401819), Lisa (UF0401830), Taragvi (UF0401864), Visamo (1–356)/L. monnseo (UF0402178), Ручеек (UF0401897), Lirina (UF0401900), Опус (UF0402142), СКі‑1 (UF0402143), Ruta (UF0402228) samples. The frequency of callusogenesis was 15.0 – 100 %, the calus mass from one explant was 0.56 – 1.51 g, the frequency of organogenesis was 10.0 – 93.8 %, the number of shoots was 1.4 – 4.0 pieces and the height of the shoots was 0.78 – 2.37 cm. Conclusions. Collection samples of Глінум (Ukraine), Кром (Russia), Visamo (1‑356)/L. monnseo (Czech Republic), Ручеек (Russia) and СКі‑1 (USA) were the best of complex signs (callusogenesis frequency, organogenesis frequency and number of shoots). Elongata flax and humile flax are characterized by the highest frequency of callusogenesis and organogenesis on hypocotyl and epicotyl explants, intermedia flax forms the largest mass of callus from the explant, the number of regenerated shoots and their height, intermedia flax has the greatest variation of the studied signs.

EXPANSION OF HEMATOPOIETIC STEM CELLS IN VITRO AS A MODEL SYSTEM FOR HUMAN TISSUE ENGINEERING

2000 ◽  
Vol 31 (3) ◽  
pp. 499-509 ◽  
Author(s):  
Joel S. Greenberger ◽  
Julie P. Goff ◽  
Jason Bush ◽  
Alfred Bahnson ◽  
Douglas Koebler ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Human Tissue ◽  
Model System ◽  
Hematopoietic Stem

Organogenesis in vitro and hyperhydricity in relation to pH of the medium and nitrogen uptake

1996 ◽  
Vol 130 (4-6) ◽  
pp. 948-950
Author(s):  
G. Pasqua ◽  
B. Monacelli ◽  
S. Anselmi ◽  
F. Manes
Keyword(s):  
Nitrogen Uptake ◽  
Organogenesis In Vitro

scholarly journals Exposure of ichnovirus particles to digitonin leads to enhanced infectivity and induces fusion from without in an in vitro model system

2007 ◽  
Vol 88 (11) ◽  
pp. 2977-2984 ◽  
Author(s):  
Don Stoltz ◽  
Renée Lapointe ◽  
Andrea Makkay ◽  
Michel Cusson
Keyword(s):  
Outer Membrane ◽  
In Vitro Model ◽  
Model System ◽  
Host Cells ◽  
Fusion Event ◽  
Susceptible Host ◽  
In Vitro Model System ◽  
Vitro Model

Unlike most viruses, the mature ichnovirus particle possesses two unit membrane envelopes. Following loss of the outer membrane in vivo, nucleocapsids are believed to gain entry into the cytosol via a membrane fusion event involving the inner membrane and the plasma membrane of susceptible host cells; accordingly, experimentally induced damage to the outer membrane might be expected to increase infectivity. Here, in an attempt to develop an in vitro model system for studying ichnovirus infection, we show that digitonin-induced disruption of the virion outer membrane not only increases infectivity, but also uncovers an activity not previously associated with any polydnavirus: fusion from without.

Effect of Oxidized Myofibrils Protein Subjected to Mutiple Freeze-Thaw Cycles on N-Nitrosamine Formation in In Vitro Model System

2012 ◽  
Vol 550-553 ◽  
pp. 1590-1594 ◽  
Author(s):  
Hua Yang ◽  
Pei Pei Meng ◽  
Rui Wang ◽  
Pei Ran Li ◽  
Peng Li ◽  
...  
Keyword(s):  
Sodium Nitrite ◽  
Protein Carbonyl ◽  
Model System ◽  
Model Systems ◽  
Secondary Amines ◽  
Freeze Thaw ◽  
Heavy Chains ◽  
Carcinogenic Substance ◽  
The Times

N-nitrosamine is a kind of carcinogenic substance, which is possibly formed in the reaction of nitrites with amino acids or secondary amines. Two in vitro model systems were designed to evaluate the influence of oxidized myofibrils protein subjected to repeated freeze-thaw cycles (0, 1, 2, 3, 4, 7, 10 times) on N-nitrosamine formation. Model system I contains diethylamine and sodium nitrite, while model system II contains only sodium nitrite as reaction solution. Oxidized myofibrils protein were added to both systems. The results revealed that as the number of freeze-thaw cycles increased, cross-linking of myosin heavy chains and the content of protein carbonyl increased, but the content of protein sulfydryl decreased, which indicates oxidization of protein occurred. The concentration of N-nitrosodiethylamine increased as the number of freeze-thaw cycles increased, especially after four cycles. Oxidized myofibrils protein promoted the formation of N-nitrosodiethylamine. The more the times of freeze-thaw cycles were subjected, the more oxidization of myofibrils protein occurred and the higher yield of the N-nitrosodiethylamine.

Attraction and penetration of Musa by the burrowing nematode, Radopholus similis, in an autotrophic bipartite culture system

Nematology ◽  
2021 ◽  
pp. 1-14
Author(s):  
Suganthagunthalam Dhakshinamoorthy ◽  
Erwin J. Galon ◽  
Annemie Elsen ◽  
Dirk De Waele
Keyword(s):  
Culture System ◽  
Model System ◽  
Radopholus Similis ◽  
Susceptible Plant ◽  
Compartment System ◽  
Female Migration ◽  
Equal Distance ◽  
Burrowing Nematode ◽  
Nematode Chemotaxis

Summary Our objective was to discover the stages (pre- or post-infection) in which the resistance to burrowing nematode (Radopholus similis) occurs in two resistant banana (Musa spp.) cultivars. An autotrophic in vitro culture system was used to compare R. similis migration towards, and penetration into, the banana roots. A new two-compartment autotrophic in vitro model system was developed using agar-based medium to examine the migration of R. similis to either the susceptible ‘Grande Naine’ or the resistant ‘Yangambi km5’ (‘Ykm5’), when both the Musa genotypes were present at equal distance. The autotrophic in vitro model system was advantageous, because it supported continuous root growth due to the actively photosynthesising shoots growing in the open air, while the in vitro root conditions make it possible to observe and assess the nematode chemotaxis in the transparent medium. Significantly fewer nematodes migrated towards the resistant ‘Ykm5’ plants when compared to both the susceptible ‘Grande Naine’, and another resistant cultivar, ‘Saba’, at 1 h after infection. This signals a possibility of a lower concentration or different composition of nematode attractants in ‘Ykm5’ root exudates. No significant differences were observed in the percentage of R. similis that migrated towards the roots of the susceptible and resistant banana plants at 3, 4 and 6 h after inoculation. No significant differences were observed in the percentages of female penetration in the resistant and susceptible plant roots at 1 and 2 days after inoculation. The results of the two-compartment system confirmed that when a choice is given to migrate towards the resistant and susceptible genotypes, no differences were observed in the percentage of female migration towards both the genotypes.

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