Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

1993 ◽  
Vol 15 (12) ◽  
pp. 1217-1222 ◽  
Author(s):  
Kenji Tanaka ◽  
Kouji Katamune ◽  
Ayaaki Ishizaki
Keyword(s):  
Lactococcus Lactis ◽  
Culture Method ◽  
Alcaligenes Eutrophus ◽  
Hydroxybutyric Acid ◽  
Two Stage ◽  
Fermentative Production ◽  
Two Stage Culture

Fermentative production of poly(β-hydroxybutyric acid) from xylose via L-lactate by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

1995 ◽  
Vol 41 (13) ◽  
pp. 257-261 ◽  
Author(s):  
Kenji Tanaka ◽  
Kouji Katamune ◽  
Ayaaki Ishizaki
Keyword(s):  
Growth Rate ◽  
Lactic Acid ◽  
Lactococcus Lactis ◽  
Batch Culture ◽  
Lactate Concentration ◽  
Culture Method ◽  
Alcaligenes Eutrophus ◽  
Hydroxybutyric Acid ◽  
Two Stage ◽  
Two Stage Culture

A two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus was developed for the production of polyhydroxyalkanoic acid (PHA) from xylose via L-lactate. In this culture method, xylose was converted into L-lactic acid and acetic acid by a culture of L. lactis IO-1, and the organic acids were then converted into PHA by a culture of A. eutrophus. Alcaligenes eutrophus grew at a specific growth rate of 0.61/h; however, the growth rate decreased as the lactate concentration in the medium increased. When the supernatant of the IO-1 culture broth containing 10-g/L L-lactate was used as a medium for A. eutrophus in batch culture, the cell concentration increased to 8.5 g/L in 24 h and 55% (w/w) of the content of the cells was found to be poly(β-hydroxybutyric acid), P(3HB). Furthermore, fed-batch culture of A. eutrophus was carried out with feeding of L-lactic acid to maintain the L-lactate concentration around 3.0 g/L. As a result, 41.0 g/L of cells and 28.7 g/L of P(3HB) were produced after 17 h of cultivation.Key words: polyhydroxybutyric acid, xylose, L-lactic acid, Alcaligenes eutrophus, Lactococcus lactis.

scholarly journals A Two-Stage Culture Method for Zygotic Embryos Effectively Overcomes Constraints Imposed by Hypocotyl and Epicotyl Seed Dormancy in Paeonia ostii ‘Fengdan’

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 356
Author(s):  
Ren ◽  
Liu ◽  
Jeong
Keyword(s):  
Red Light ◽  
Culture Method ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Dormancy Breaking ◽  
Two Stage ◽  
Light Emitting ◽  
Constraint Factors ◽  
Negative Effect ◽  
Two Stage Culture

The effect of the exogenous hormone and light quality on breaking hypocotyl and epicotyl dormancy was studied. The results showed that the greatest percentage of hypocotyl dormancy breaking was observed with the Murashige and Skoog (MS) medium supplemented with or without 1.0 mg·L−1 gibberellin 3 (GA3), while ABA and endosperm greatly inhibited hypocotyl dormancy breaking. This suggests that hypocotyl dormancy of the Paeonia ostii ‘Fengdan’ embryo could be easily overcome by removing constraints of the surrounding endosperm, and ABA may be one of the constraint factors contained in the endosperm. The percentage of epicotyl dormancy breaking was also greatly affected by the concentration of 6-benzylaminopurine (BA) and GA3. Compared to BA by itself, adding GA3 to the medium containing BA highly enhanced epicotyl dormancy breaking, with the greatest percentage of epicotyl dormancy breaking in MS medium supplemented with both 0.5 mg·L−1 BA and 0.5–1.0 mg·L−1 GA3. The percentage of hypocotyl and epicotyl dormancy breaking was also affected by light and its quality. Red light-emitting diodes (LEDs) had the same effect as a dark condition on the hypocotyl dormancy breaking, while blue LEDs and a combination of red and blue LEDs had a negative effect on the hypocotyl dormancy breaking. Unexpectedly, blue LEDs greatly enhanced, whereas red LEDs inhibited, epicotyl dormancy breaking. Conclusively, a two-stage culture method was recommended for breaking the hypocotyl and epicotyl dormancy: hypocotyl dormancy was broken first using the MS medium without any plant growth regulators in the dark (25 °C), and epicotyl dormancy was subsequently broken with the MS medium supplemented with both 1.0 mg·L−1 GA3 and 0.5 mg·L−1 BA under blue light.

scholarly journals Scopolamíne Yield in Cultured Roots ofDuboisia myoporoidesImproved by a Novel Two-stage Culture Method

1994 ◽  
Vol 58 (10) ◽  
pp. 1820-1823 ◽  
Author(s):  
Yukihito Yukimune ◽  
Homare Tabata ◽  
Yasuhiro Hara ◽  
Yasuyuki Yamada
Keyword(s):  
Culture Method ◽  
Two Stage ◽  
Two Stage Culture

Hybrid two-stage culture of Halamphora coffeaeformis for biodiesel production: Growth phases, nutritional stages and biorefinery approach

2018 ◽  
Vol 118 ◽  
pp. 984-992 ◽  
Author(s):  
Lucas A. Martín ◽  
Cecilia A. Popovich ◽  
Ana M. Martínez ◽  
Paola G. Scodelaro Bilbao ◽  
María C. Damiani ◽  
...  
Keyword(s):  
Biodiesel Production ◽  
Growth Phases ◽  
Two Stage ◽  
Production Growth ◽  
Two Stage Culture
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