scholarly journals A Two-Stage Culture Method for Zygotic Embryos Effectively Overcomes Constraints Imposed by Hypocotyl and Epicotyl Seed Dormancy in Paeonia ostii ‘Fengdan’

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 356
Author(s):  
Ren ◽  
Liu ◽  
Jeong
Keyword(s):  
Red Light ◽  
Culture Method ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Dormancy Breaking ◽  
Two Stage ◽  
Light Emitting ◽  
Constraint Factors ◽  
Negative Effect ◽  
Two Stage Culture

The effect of the exogenous hormone and light quality on breaking hypocotyl and epicotyl dormancy was studied. The results showed that the greatest percentage of hypocotyl dormancy breaking was observed with the Murashige and Skoog (MS) medium supplemented with or without 1.0 mg·L−1 gibberellin 3 (GA3), while ABA and endosperm greatly inhibited hypocotyl dormancy breaking. This suggests that hypocotyl dormancy of the Paeonia ostii ‘Fengdan’ embryo could be easily overcome by removing constraints of the surrounding endosperm, and ABA may be one of the constraint factors contained in the endosperm. The percentage of epicotyl dormancy breaking was also greatly affected by the concentration of 6-benzylaminopurine (BA) and GA3. Compared to BA by itself, adding GA3 to the medium containing BA highly enhanced epicotyl dormancy breaking, with the greatest percentage of epicotyl dormancy breaking in MS medium supplemented with both 0.5 mg·L−1 BA and 0.5–1.0 mg·L−1 GA3. The percentage of hypocotyl and epicotyl dormancy breaking was also affected by light and its quality. Red light-emitting diodes (LEDs) had the same effect as a dark condition on the hypocotyl dormancy breaking, while blue LEDs and a combination of red and blue LEDs had a negative effect on the hypocotyl dormancy breaking. Unexpectedly, blue LEDs greatly enhanced, whereas red LEDs inhibited, epicotyl dormancy breaking. Conclusively, a two-stage culture method was recommended for breaking the hypocotyl and epicotyl dormancy: hypocotyl dormancy was broken first using the MS medium without any plant growth regulators in the dark (25 °C), and epicotyl dormancy was subsequently broken with the MS medium supplemented with both 1.0 mg·L−1 GA3 and 0.5 mg·L−1 BA under blue light.

Fermentative production of poly(β-hydroxybutyric acid) from xylose via L-lactate by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

1995 ◽  
Vol 41 (13) ◽  
pp. 257-261 ◽  
Author(s):  
Kenji Tanaka ◽  
Kouji Katamune ◽  
Ayaaki Ishizaki
Keyword(s):  
Growth Rate ◽  
Lactic Acid ◽  
Lactococcus Lactis ◽  
Batch Culture ◽  
Lactate Concentration ◽  
Culture Method ◽  
Alcaligenes Eutrophus ◽  
Hydroxybutyric Acid ◽  
Two Stage ◽  
Two Stage Culture

A two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus was developed for the production of polyhydroxyalkanoic acid (PHA) from xylose via L-lactate. In this culture method, xylose was converted into L-lactic acid and acetic acid by a culture of L. lactis IO-1, and the organic acids were then converted into PHA by a culture of A. eutrophus. Alcaligenes eutrophus grew at a specific growth rate of 0.61/h; however, the growth rate decreased as the lactate concentration in the medium increased. When the supernatant of the IO-1 culture broth containing 10-g/L L-lactate was used as a medium for A. eutrophus in batch culture, the cell concentration increased to 8.5 g/L in 24 h and 55% (w/w) of the content of the cells was found to be poly(β-hydroxybutyric acid), P(3HB). Furthermore, fed-batch culture of A. eutrophus was carried out with feeding of L-lactic acid to maintain the L-lactate concentration around 3.0 g/L. As a result, 41.0 g/L of cells and 28.7 g/L of P(3HB) were produced after 17 h of cultivation.Key words: polyhydroxybutyric acid, xylose, L-lactic acid, Alcaligenes eutrophus, Lactococcus lactis.

scholarly journals Scopolamíne Yield in Cultured Roots ofDuboisia myoporoidesImproved by a Novel Two-stage Culture Method

1994 ◽  
Vol 58 (10) ◽  
pp. 1820-1823 ◽  
Author(s):  
Yukihito Yukimune ◽  
Homare Tabata ◽  
Yasuhiro Hara ◽  
Yasuyuki Yamada
Keyword(s):  
Culture Method ◽  
Two Stage ◽  
Two Stage Culture

scholarly journals Understanding the luminescent nature of organic radicals for efficient doublet emitters and pure-red light-emitting diodes

2020 ◽  
Vol 19 (11) ◽  
pp. 1224-1229 ◽  
Author(s):  
Alim Abdurahman ◽  
Timothy J. H. Hele ◽  
Qinying Gu ◽  
Jiangbin Zhang ◽  
Qiming Peng ◽  
...  
Keyword(s):  
Light Emitting Diodes ◽  
Red Light ◽  
Organic Radicals ◽  
Light Emitting

scholarly journals Red Light-Emitting Diodes with All-Inorganic CsPbI3/TOPO Composite Nanowires Color Conversion Films

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Lung-Chien Chen ◽  
Yi-Tsung Chang ◽  
Ching-Ho Tien ◽  
Yu-Chun Yeh ◽  
Zong-Liang Tseng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Light Emitting Diode ◽  
Red Light ◽  
Trioctylphosphine Oxide ◽  
Optical And Electrical Properties ◽  
Colloidal Solutions ◽  
High Quality ◽  
Light Emitting ◽  
Hot Injection ◽  
Color Conversion

AbstractThis work presents a method for obtaining a color-converted red light source through a combination of a blue GaN light-emitting diode and a red fluorescent color conversion film of a perovskite CsPbI3/TOPO composite. High-quality CsPbI3 quantum dots (QDs) were prepared using the hot-injection method. The colloidal QD solutions were mixed with different ratios of trioctylphosphine oxide (TOPO) to form nanowires. The color conversion films prepared by the mixed ultraviolet resin and colloidal solutions were coated on blue LEDs. The optical and electrical properties of the devices were measured and analyzed at an injection current of 50 mA; it was observed that the strongest red light intensity was 93.1 cd/m2 and the external quantum efficiency was 5.7% at a wavelength of approximately 708 nm when CsPbI3/TOPO was 1:0.35.

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