Kinetics of wheat straw solid-state fermentation with Trametes versicolor and Pleurotus ostreatus ? lignin and polysaccharide alteration and production of related enzymatic activities

1991 ◽  
Vol 35 (6) ◽  
Author(s):  
Manuel Valmaseda ◽  
Mar�aJes�s Mart�nez ◽  
AngelT. Mart�nez
Keyword(s):  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
Enzymatic Activities ◽  
Kinetics Of

Solid-State Fermentation of Rapeseed Meal with the White-Rot Fungi Trametes versicolor and Pleurotus ostreatus

2013 ◽  
Vol 171 (8) ◽  
pp. 2075-2081 ◽  
Author(s):  
Jerzy Żuchowski ◽  
Łukasz Pecio ◽  
Magdalena Jaszek ◽  
Anna Stochmal
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
White Rot Fungi ◽  
Rapeseed Meal ◽  
White Rot

scholarly journals Production and characterization of cellulase from mushroom (Pleurotus ostreatus) for effective degradation of cellulose

2021 ◽  
Vol 2 (1) ◽  
pp. 135-150
Author(s):  
Beyisa Benti Diro ◽  
Tadessa Daba ◽  
Temam Gemeda Genemo
Keyword(s):  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Pleurotus Ostreatus ◽  
Wood Fiber ◽  
Cellulase Production ◽  
Tree Bark ◽  
Lignocellulosic Substrates ◽  
Optimum Ph

Cellulases are a group of hydrolytic enzymes capable of hydrolyzing the most abundant organic polymer that means cellulose to smaller sugar components including glucose subunits. The aim of this study was to screen cellulase producing oyster mushroom collected from Eucalyptus tree bark to evaluate the in vitro production of cellulase by Pleurotus ostreatus using different lignocellulosic substrates, and to characterize the cellulase produced with respect to changes in pH, temperature, and concentration of substrates. A total of ten mushroom specimens were randomly collected from Eucalyptus tree bark in the premise of Holetta Agricultural Research Center campus. All of the collected mushroom specimens were identified morphologically and biochemically as Pleurotus ostreatus and also screened for their ability to produce cellulase by detecting and measuring zone of hydrolysis on commercial media containing Carbxymethyl Cellulose (CMC) as the sole carbon source. These mushroom specimens were cultivated using both solid state fermentation and submerged fermentation systems supplemented with different lignocellulosic substrates (wheat straw, teff straw, bean straw, wood fiber and Eucalyptus tree bark) to identify the most suitable medium for the production of cellulase. The highest enzyme production was obtained on bean straw and wheat straw which resulted in 0.191 U/ml, 0.868 U/ml and 0.389 U/ml; and 0.216 U/ml, 0.444 U/ml, and 0.245 U/ml of FPase, CMCase, and β-glucosidase in solid state fermentation. The lowest values were, however, obtained in media containing wood fiber in both solid state fermentation and submerged fermentation. Comparison of the lignocellulosic substrates revealed that wheat straw was selected for further growth parameter optimization. The production of cellulase was higher at the 5th day of incubation period, and the optimum pH and incubation temperature required for maximum cellulase production were 4 and 30°C, respectively. Sucrose and Yeast extract at 1% concentration were found to be the most preferred carbon and nitrogen sources for cellulase production by Pleurotus ostreatus. The optimum pH and temperature for cell_free cellulase activity on were found to be 4 and 50°C, respectively. Generally the cellulases produced by Pleurotus ostreatus were stable and active at temperatures ranging from 20-50°C. These characteristics hopefully would make this enzyme potentially attractive in a variety of industrial applications including animal feed treatments. There was a linear relationship between cellulase and its substrate concentration for there was an increase in activity with increase in substrate concentration. The relationship between rate of reaction and substrate concentration depended on the affinity of the enzyme for its substrate. Finally the cellulase was tested for its ability to saccharify agricultural wastes and the results showed the highest release of sugars from wheat straw.

scholarly journals Trametes suaveolens as ligninolytic enzyme producer

2013 ◽  
pp. 437-444 ◽  
Author(s):  
Aleksandar Knezevic ◽  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jelena Vukojevic
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Nitrogen Concentration ◽  
Pollution Prevention ◽  
Ligninolytic Enzyme ◽  
Species Selection ◽  
Submerged Cultivation ◽  
Phenol Red

Species of the genus Trametes represent one of the most efficient lignin-degraders which can be attributed to a well developed ligninolytic enzyme system. Current trends are screening of ability of new species to produce these enzymes, as well as the optimization of conditions for their overproduction. Therefore, the aim of the study was to evaluate the potential of T. suaveolens to synthesize laccase and Mn-oxidizing peroxidases during fermentation of the selected plant raw materials. Level of enzyme activities was measured on 7, 10 and 14th day of submersion, as well as the solid-state fermentation of wheat straw and oak sawdust in the presence of NH4NO3 in previously determined optimal nitrogen concentration of 25 mM. The enzyme activity was determined spectrophotometrically using ABTS and phenol red as the substrates. The highest level of laccase activity (1087.1 U/L) was noted after 7 days of wheat straw solid-state fermentation, while during the submerged cultivation the production of the enzyme was not noted. Submerged cultivation in oak sawdust-enriched medium was the optimal for activity of Mn-dependent peroxidase (1767.7 U/L on day 14) and Mn-independent peroxidase (1113.7 U/L on day 7). Introduction of T. suaveolens to produce ligninolytic enzyme represented the base for further study, as well as the determination of relation between enzyme activity and rate of lignin degradation. It could lead to greater possibility of fungal species selection with high delignification capacity, which could take participation in sustainable production of food, feed, fibres, and energy, environmentally friendly pollution prevention, and bioremediation.

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