In vitro experiment and initial clinical experience in angioplasty, in vascular occlusive disease with a new pulsed dye-laser system

BackgroundThe optimal interaction between stent struts and thrombus is crucial for successful revascularization in endovascular stroke therapy with stent retrievers. Deploying the stent retriever by actively pushing it into the thrombus increases the radial force with which the stent struts expand into the thrombus.ObjectiveTo examine the active push deployment (APD) technique in an in vitro model and present our clinical experience with this technique.MethodsIn an in vitro experiment we investigated the configuration of a Solitaire and a Trevo ProVue device (both 4×20 mm), depending on whether the devices were deployed using the APD technique or simple unsheathing. We retrospectively assessed the effectiveness and safety of this technique by analyzing 130 patients with large vessel occlusions (carotid T or M1 segment of the middle cerebral artery), who received endovascular treatment with a Trevo device (4×20 mm) that was deployed using the APD technique.ResultsIn vitro experiment: the APD technique improved apposition of the devices to the vessel wall. There was widening of 30% (Trevo) and 19% (Solitaire) at the cost of a shortening of 5% and 4%, respectively, when the devices were deployed in a carotid T model. Clinical study: the revascularization rate (Thrombolysis in Cerebral Infarction ≥2b) with the Trevo device was 90%. There were no retriever-associated dissections or perforations in 278 retrieval maneuvers.ConclusionsThe APD technique improves apposition of the tested devices to the vessel wall. The widening effect comes at the cost of minimal shortening of the devices. Our clinical experience shows that using the APD technique to deploy the Trevo device is effective and safe.

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IN VITRO EXPERIMENT STUDY ON ABLATION PARAMETERS IN FEMTOSECOND LASER-OCULAR TISSUE INTERACTION

International Journal of Nanoscience ◽

10.1142/s0219581x05003292 ◽

2005 ◽

Vol 04 (04) ◽

pp. 483-488

Author(s):

K. A. NGOI ◽

D. X. HOU ◽

S. T. HOH ◽

L. H. K. KOH ◽

Y. Z. DENG

Keyword(s):

Femtosecond Laser ◽

Laser System ◽

Pulse Length ◽

Ocular Tissue ◽

Vitro Experiment ◽

In Vitro Experiment ◽

Tissue Interaction ◽

Cutting Effect ◽

Relationship Of

In order to apply femtosecond laser technology in ophthalmology, especially in glaucoma treatment, femtosecond laser-ocular tissue interaction research was conducted. Focusing angle, output power, irradiance duration are three important laser parameters for ophthalmic microsurgery. In our work, the relationship of the three different laser parameter versus cutting effect was investigated respectively by an in-vitro experiment using an 800 nm-wavelength 150 fs-pulse-length laser system. This experiment aims to minimize the effects of thermal damage, while at the same time maximizing the speed or quality of the tissue ablation process. The experiment result shows that there is a great potential to optimize the surgical effects using femtosecond laser compared to that using a longer pulse laser.

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Pulsed Dye Laser Fragmentation of Ureteral Calculi: Initial Clinical Experience

The Journal of Urology ◽

10.1016/s0022-5347(17)44043-2 ◽

1987 ◽

Vol 137 (3) ◽

pp. 386-389 ◽

Cited By ~ 150

Author(s):

Stephen P. Dretler ◽

Graham Watson ◽

John A. Parrish ◽

Steven Murray

Keyword(s):

Clinical Experience ◽

Dye Laser ◽

Pulsed Dye Laser ◽

Ureteral Calculi ◽

Initial Clinical Experience

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Pulsed Dye Laser Lithotripsy for Ureteral Calculi: Initial Clinical Experience

The Journal of Urology ◽

10.1016/s0022-5347(17)75871-5 ◽

1987 ◽

Vol 137 (6) ◽

Author(s):

Theodore V. Benderev ◽

Donald C. Martin ◽

Michael W. Berns

Keyword(s):

Clinical Experience ◽

Laser Lithotripsy ◽

Dye Laser ◽

Pulsed Dye Laser ◽

Ureteral Calculi ◽

Initial Clinical Experience

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In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

Current Proteomics ◽

10.2174/1570164616666190131150033 ◽

2019 ◽

Vol 16 (4) ◽

pp. 307-313 ◽

Cited By ~ 2

Author(s):

Nasrin Zarkar ◽

Mohammad Ali Nasiri Khalili ◽

Fathollah Ahmadpour ◽

Sirus Khodadadi ◽

Mehdi Zeinoddini

Keyword(s):

In Silico ◽

Catalytic Domain ◽

Md Simulations ◽

Special Importance ◽

Native Form ◽

Vitro Experiment ◽

In Vitro Experiment ◽

Pharmaceutical Protein ◽

The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

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An in vitro experiment on the interaction of charcoal or wheat bran with 11-nor-9-carboxy-Δ9-tetrahydrocannabinol and its glucuronide

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-013-7381-4 ◽

2013 ◽

Vol 405 (29) ◽

pp. 9449-9453

Author(s):

Gisela Skopp ◽

Gerd Mikus

Keyword(s):

Wheat Bran ◽

Vitro Experiment ◽

In Vitro Experiment

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Application of the 1-µsec pulsed-dye laser to the treatment of experimental cerebral vasospasm

Journal of Neurosurgery ◽

10.3171/jns.1991.75.2.0271 ◽

1991 ◽

Vol 75 (2) ◽

pp. 271-276 ◽

Cited By ~ 12

Author(s):

Atsushi Teramura ◽

Robert Macfarlane ◽

Christopher J. Owen ◽

Ralph de la Torre ◽

Kenton W. Gregory ◽

...

Keyword(s):

Cerebral Vasospasm ◽

Basilar Artery ◽

Laser Energy ◽

Autologous Blood ◽

Preliminary Investigation ◽

Dye Laser ◽

Pulsed Dye Laser ◽

Content Type

✓ Laser energy of 480 nm was applied in 1-µsec pulses varying between 2.2 and 10 mJ to in vitro and in vivo models of cerebral vasospasm. First, the pulsed-dye laser was applied intravascularly via a 320-µm fiber to basilar artery segments from six dogs. The segments were mounted in a vessel-perfusion apparatus and constricted to, on average, 70% of resting diameter by superfusion with dog hemolysate. Immediate increase in basilar artery diameter occurred to a mean of 83% of control. In a second model, the basilar artery was exposed transclivally in the rabbit. In three normal animals, superfusion of the artery with rabbit hemolysate resulted in a reduction of mean vessel diameter to 81% of control. Following extravascular application of the laser, vessels returned to an average of 106% of the resting state. In six rabbits, the basilar artery was constricted by two intracisternal injections of autologous blood, 3 days apart. Two to 4 days after the second injection, the basilar artery was exposed. Extravascular laser treatment from a quartz fiber placed perpendicular to the vessel adventitia resulted in an immediate 53% average increase in caliber to an estimated 107% of control. No reconstriction was observed over a period of up to 5 hours. Morphologically, damage to the arterial wall was slight. This preliminary investigation suggests that the 1-µsec pulsed-dye laser may be of benefit in the treatment of cerebral vasospasm.

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