Time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by Fusarium oxysporum f. sp. radicis-lycopersici

Planta ◽  
1991 ◽  
Vol 184 (2) ◽  
pp. 196-208 ◽  
Author(s):  
Nicole Benhamou ◽  
Dominique Mazau ◽  
Jean Grenier ◽  
Marie-Thérése Esquerré-Tugayé
1991 ◽  
Vol 69 (6) ◽  
pp. 1265-1274 ◽  
Author(s):  
Hélène Chamberland ◽  
G. B. Ouellette ◽  
F. J. Pauzé ◽  
P. M. Charest

Polyclonal antibodies produced against a purified commercial pectinesterase obtained from tomato plants permitted ultrastructural localization of this enzyme in the roots of young tomato plants infected by Fusarium oxysporum f.sp. radicis-lycopersici Jarvis & Shoemaker. In inoculated susceptible cultivars, gold labeling for pectinesterase was intense over electrondense material in the cortex intercellular spaces, in altered pit membranes in the vascular cylinder, and in the dense coating material deposited along vessel walls. Contrarily, in resistant or uninoculated susceptible plants, which lacked coating material in vessels, labelling was present but nonabundant in the intercellular spaces. The enzyme was not found in the hyphae, indicating that the labelled pectinesterase was probably of host origin. This study suggests the involvement of the tomato pectinesterase in the host reaction against infection by F. oxysporum f.sp. radicis-lycopersici.Key words: pectinesterase, immunocytochemistry, Fusarium oxysporum f.sp. radicis-lycopersici, tomato.


Genetics ◽  
1999 ◽  
Vol 153 (3) ◽  
pp. 1435-1444
Author(s):  
Yin-Zhou Zheng ◽  
Robin R Roseman ◽  
Wayne R Carlson

Abstract The B chromosome of maize has been used in a study of dicentric chromosomes. TB-9Sb is a translocation between the B and chromosome 9. The B-9 of TB-9Sb carries 60% of the short arm of 9. For construction of dicentrics, a modified B-9 chromosome was used, B-9-Dp9. It consists of the B-9 chromosome plus a duplicated 9S region attached to the distal end. In meiosis, fold-back pairing and crossing over in the duplicated region gives a chromatid-type dicentric B-9 that subsequently initiates a chromatid-type breakage-fusion-bridge cycle. In the male, it forms a single bridge in anaphase II of meiosis and at the first pollen mitosis. However, the cycle is interrupted by nondisjunction of the B centromere at the second pollen mitosis, which sends the B-9 dicentric to one pole and converts it from a chromatid dicentric to a chromosome dicentric. As expected, the new dicentric undergoes the chromosome-type breakage-fusion-bridge cycle and produces double bridges. A large number of plants with chromosome dicentrics were produced in this way. The presence of double bridges in the root cells of plants with a chromosome dicentric was studied during the first 10 wk of development. It was found that the number of plants and cells showing double bridges declined steadily over the 10-wk period. Several lines of evidence indicate that there was no specific developmental time for dicentric loss. “Healing” of broken chromosomes produced by dicentric breakage accounted for much of the dicentric loss. Healing produced a wide range of derived B-9 chromosomes, some large and some small. A group of minichromosomes found in these experiments probably represents the small end of the scale for B-9 derivatives.


Hypertension ◽  
1980 ◽  
Vol 2 (4) ◽  
pp. 102-108 ◽  
Author(s):  
A. W. Voors ◽  
L. S. Webber ◽  
G. S. Berenson

Author(s):  
Cristiana Maia de Oliveira ◽  
Margarida Gorete Ferreira do Carmo ◽  
Leandro Martins Ferreira ◽  
Monica Höfte ◽  
Nelson Moura Brasil do Amaral Sobrinho

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1818
Author(s):  
Francisco Hernández-Aparicio ◽  
Purificación Lisón ◽  
Ismael Rodrigo ◽  
José María Bellés ◽  
M. Pilar López-Gresa

New strategies of control need to be developed with the aim of economic and environmental sustainability in plant and crop protection. Metabolomics is an excellent platform for both understanding the complex plant–pathogen interactions and unraveling new chemical control strategies. GC-MS-based metabolomics, along with a phytohormone analysis of a compatible and incompatible interaction between tomato plants and Fusarium oxysporum f. sp. lycopersici, revealed the specific volatile chemical composition and the plant signals associated with them. The susceptible tomato plants were characterized by the over-emission of methyl- and ethyl-salicylate as well as some fatty acid derivatives, along with an activation of salicylic acid and abscisic acid signaling. In contrast, terpenoids, benzenoids, and 2-ethylhexanoic acid were differentially emitted by plants undergoing an incompatible interaction, together with the activation of the jasmonic acid (JA) pathway. In accordance with this response, a higher expression of several genes participating in the biosynthesis of these volatiles, such as MTS1, TomloxC,TomloxD, and AOS, as well as JAZ7, a JA marker gene, was found to be induced by the fungus in these resistant plants. The characterized metabolome of the immune tomato plants could lead to the development of new resistance inducers against Fusarium wilt treatment.


2012 ◽  
Vol 19 (6) ◽  
pp. 367-376 ◽  
Author(s):  
Viktoriya Golovatscka ◽  
Helena Ennes ◽  
Emeran A. Mayer ◽  
Sylvie Bradesi

2005 ◽  
Vol 15 (4) ◽  
pp. 474-481 ◽  
Author(s):  
Kusal Wickremesekera ◽  
Geoff Miller ◽  
Tissa DeSilva Naotunne ◽  
Graham Knowles ◽  
Richard S. Stubbs

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