An electron microscopic radioautographic study of the uptake of tritiated serotonin by nerve fibres in the posterior salivary duct and gland of cephalopods

1975 ◽  
Vol 161 (3) ◽  
Author(s):  
Colette Ducros
Development ◽  
1987 ◽  
Vol 100 (2) ◽  
pp. 307-311 ◽  
Author(s):  
A.M. Davies

I have used a novel quantitative electron microscopic method to determine the rate at which nerve fibres grow towards their targets during development. The rate of recruitment of nerve fibres to the maxillary nerve of the mouse embryo was determined by counting the number of axon profiles in the nerve sectioned close to its emergence from the trigeminal ganglion at closely staged intervals throughout its early development. The rate of change of fibre number with distance along this nerve was determined by counting the number of axon profiles at intervals along the nerve at stages during the period that fibres are growing to their targets. From these two parameters, both of which were linear functions during the midperiod of fibre recruitment to the nerve, it has been possible to calculate that embryonic sensory nerve fibres grow to their peripheral targets at the surprisingly slow rate of just over 20 micron h-1.


1997 ◽  
Vol 25 (6) ◽  
pp. 354-358 ◽  
Author(s):  
Y Fukuda ◽  
M Imoto ◽  
I Nakano ◽  
Y Katano ◽  
T Hayakawa

Noradrenergic innervation of rat liver was studied immunohistochemically using antibody to noradrenaline at the electron-microscopic level. Noradrenaline-immunoreactive nerve fibres were located in the portal tract and some were in close contact with the portal vein and hepatic artery. Noradrenaline-immunoreactive fibres were found to contain many vesicles that were reactive to anti-noradrenaline antibody. This preliminary study suggests that the method for detecting noradrenaline-immunoreactive fibres using the antibody is useful for studies at the electron-microscopic level.


1974 ◽  
Vol 14 (3) ◽  
pp. 657-669
Author(s):  
CARYL E. HILL ◽  
JULIE H. CHAMLEY ◽  
G. BURNSTOCK

Sympathetic ganglia from newborn rats and guinea-pigs were grown in modified Rose chambers and examined with scanning electron microscopy after 5-7 days. The cell types seen were macrophages, neurons, glial cells and connective tissue cells. They presented a wide range of surface morphologies and 3-dimensional configurations, from spheroid with an irregular surface to flattened with a smooth surface. The arrangement of the nerve fibres and cells in the outgrowth was essentially 2-layered with connective tissue cells nearest the substrate and nerve fibres, glial cells and macrophages lying over them. The relationships of sympathetic nerve fibres to the different cell types were also investigated. In all cases nerve fibres closely followed the cellular surface contours although the nature of the relationships varied. Fine finger-like cytoplasmic projections were sometimes seen from connective tissue cells and macrophages. The possible role of these structures in adhesion and motility is discussed.


1976 ◽  
Vol 173 (4) ◽  
pp. 461-464 ◽  
Author(s):  
A. Van Vossel ◽  
K. Dierickx ◽  
F. Vandesande ◽  
J. Van Vossel-Daeninck

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