A Quantitative Analytical Method for the Identification and Characterization of Mineralized Nanoparticles in Food Supplements

Food supplements play an increasing role in the nanofood market because improvement of effectiveness and efficiency of delivery of bioactive compounds is presumed. An electron microscopic method based on the international Standard ISO 13322-1:2004 was developed for controlling and documenting size, size distribution, surface structure, shape and aggregation state of mineralized food supplement particles. For particle size distribution, a combination of conventional TEM- and SEM-methods, which allows identifying the size distribution of the whole product, is described.

A Histochemical Approach to Correlative Light and Electron Microscopic Detection of Acidic Glycoconjugates by a Sensitized High Iron Diamine Method

A sensitized high iron diamine method is among the reliable and useful histochemical means of detecting acidic glycoconjugates by light microscopy. Because the final reaction products obtained using this method are heavy metals, it can be applied to specimens for visualization by both light and electron microscopy. In this study the high iron diamine method was utilized successfully as a correlative light and electron microscopic method for detection of acidic glycoconjugates.