The fine structure and phosphatase cytochemistry of the Golgi complex and associated structures in the Sertoli cells of Syrian hamsters

1975 ◽  
Vol 157 (2) ◽  
Author(s):  
I-Li Chen ◽  
RobertD. Yates
Keyword(s):  
Fine Structure ◽  
Golgi Complex ◽  
Sertoli Cells ◽  
Syrian Hamsters ◽  
Phosphatase Cytochemistry

scholarly journals PERMANGANATE FIXATION OF THE GOLGI COMPLEX AND OTHER CYTOPLASMIC STRUCTURES OF MAMMALIAN TESTES

1960 ◽  
Vol 8 (3) ◽  
pp. 761-775 ◽  
Author(s):  
Hilton H. Mollenhauer ◽  
William Zebrun
Keyword(s):  
Fine Structure ◽  
Guinea Pig ◽  
Golgi Complex ◽  
Sertoli Cells ◽  
Close Association ◽  
High Electron ◽  
Cell Organelles ◽  
Different Dimensions ◽  
Morphological Differences ◽  
Cytoplasmic Structures

Observations on the fine structure of KMnO4-fixed testes of small mammals (guinea pig, rat, and mouse) reveal certain morphological differences between the spermatogenic and Sertoli cells which have not been demonstrated in the same tissue fixed with OsO4. Aggregates of minute circular profiles, much smaller than the spherical Golgi vesicles, are described in close association with the Golgi complex of developing spermatids. Groups of dense flattened vesicles, individually surrounded by a membrane of different dimensions than that which bounds most of the other cell organelles, appear dispersed within the cytoplasm of some spermatogenic cells. Flattened vesicles of greater density than those belonging to the Golgi complex are reported confined to the inner Golgi zone of developing guinea pig spermatids between the Golgi cisternae and the head cap. The profiles of endoplasmic reticulum within spermatocytes appear shorter, wider, and more tortuous than those of Sertoli cells. Minute cytoplasmic particles approximately 300 A in diameter and of high electron opacity appear randomly disposed in some Sertoli cells. Groups of irregular-shaped ovoid bodies within the developing spermatids are described as resembling portions of cytoplasm from closely adjacent spermatids. Interpretation is presented regarding the fine structure of KMnO4-fixed testes in view of what has already been reported for mammalian testes fixed in OsO4.

Demonstration of Secondary Lysosomes in Bovine Megakaryocytes and Platelets Using Acid Phosphatase Cytochemistry with Cerium as a Trapping Agent

1990 ◽  
Vol 63 (01) ◽  
pp. 127-132 ◽  
Author(s):  
Michèle Ménard ◽  
Kenneth M Meyers ◽  
David J Prieur
Keyword(s):  
Acid Phosphatase ◽  
Electron Density ◽  
Golgi Complex ◽  
Initial Report ◽  
Cerium Phosphate ◽  
Virtual Absence ◽  
Phosphatase Cytochemistry ◽  
Secondary Lysosomes ◽  
Trapping Agent

SummaryThe ultrastructure of lysosomes from bovine megakaryocytes (MK) and platelets was characterized using acid phosphatase cytochemistry with beta-glycerophosphate as substrate and cerium as a trapping agent. The technique was easily reproducible; cerium-phosphate precipitates were uniform, readily visualized, and there was a virtual absence of nonspecific reaction product. Acid phosphatase was localized in the trans aspect of the Golgi complex and/or granules of less than 50 nm to 650 nm diameters in MK at all stages of maturation. Forty percent of the MK lysosomes contained inclusions of variable shapes, sizes and electron-density and were classified as secondary lysosomes. Twenty-four percent of the platelet sections contained acid phosphatase-positive granules. Fifty-four percent of these were secondary lysosomes. This is the initial report demonstrating secondary lysosomes in either resting MK or platelets using acid phosphatase cytochemistry. These findings suggest that MK and platelet lysosomes have an intracellular function in resting MK and platelets.

Fine structure of monkey (Macaca mulatta) Sertoli cells after treatment with cyproterone

Andrologia ◽  
2009 ◽  
Vol 7 (4) ◽  
pp. 317-328 ◽  
Author(s):  
G. AUMÜLLER ◽  
B. SCHENCK ◽  
F. NEUMANN
Keyword(s):  
Fine Structure ◽  
Macaca Mulatta ◽  
Sertoli Cells

scholarly journals Fine Structure of the Sertoli Cells of the Rat Testis in Experimental Unilateral Cryptorchidism

1980 ◽  
Vol 3 (1-6) ◽  
pp. 301-311 ◽  
Author(s):  
G. Aumüller ◽  
K. Hartmann ◽  
U. Giers ◽  
B. Schenck
Keyword(s):  
Fine Structure ◽  
Sertoli Cells ◽  
Rat Testis ◽  
Unilateral Cryptorchidism

The fine structure of neurons and other elements in the nervous system of the giant African land snail Archachatina marginata

1964 ◽  
Vol 160 (979) ◽  
pp. 167-180 ◽  
Keyword(s):  
Fine Structure ◽  
Blood Vessels ◽  
Glial Cells ◽  
Golgi Complex ◽  
Land Snail ◽  
Interstitial Cells ◽  
Muscle Fibres ◽  
Nerve Fibres ◽  
Dense Granules ◽  
Sheath Cells

Much of the fine structure of the neurons, interstitial cells, blood vessels, collagen, and muscle fibres resembles that of similar elements in other species. In this preliminary report attention is paid to those features which characterize the structure of the neurons and interstitial cells. Within each group there are morphologically distinct cell types. The giant neurons (> 100 /un) are distinguished from smaller neurons by the irregular shapes of their nuclei, and by the extensive penetration of their perikarya by processes of surrounding sheath cells. A substance having a structure similar to that of glycogen is present both in the sheath cells and in the neuronal cytoplasm . The sheath cells may, therefore, be functioning as ‘nurse’ cells. The sheath cells form one group of interstitial cells. Two other groups are defined in accordance with their spatial relationships to the neurons. The glial cells are distributed between the sheath cells, where they may form a mass of glial tissue, and they are present in the walls of the blood vessels. The cytoplasm is extended into processes and in the region of the nucleus appears to be extremely active. It is characterized particularly by an abundance of the Golgi complex, small granular and large laminated electron-dense inclusions, and the particulate substance which may be glycogen. Structures which resemble the electron-dense inclusions of glial cells are found in the cytoplasm of neurons. The third group of interstitial cells consists of supporting cells which surround nerve fibres. Large fibres and particularly the neurite are deeply penetrated by these cells and the phenomenon appears to be associated with the formation of collateral nerve fibres. Dense fibrils permeate the cytoplasm and desmosomes are present between the cells, which, therefore, appear to form a resilient framework around the nerve fibres. Nerve fibres in the neuropil and nerves contain a complex array of vesicular and granular inclusions. These comprise small clear vesicles (350 to 600 A), electron-dense granules (600 to 2000 A) and a range of granular vesicles which have a structure intermediate in character betweenth at of the clear vesicles and the dense granules. The dense granules in the nerve fibres are identical with those which are present in the perikarya and which have their origin in the cisternae of the Golgi complex. A dense substance accumulates between the membranes and is concentrated in the form of a granule towards one end of a cisterna, from which it is pinched off by constriction of the mem­branes. This is probably the origin of the granules which collect in the nerve fibres. The vesicular and granular elements in the fibres are never completely segregated, although clusters occur in which one type predominates. Occasionally, in regions where the clear vesicles are prevalent, these may be associated with a thickened membrane which is apposed to a similar thickening of an adjacent nerve fibre. The whole structure closely resembles synapses which have been described in other animals. The nature of the substances located in the vesicles and granules has not been determined.

Fine structure of sertoli cells in the camel (Camelus dromedarius)

1986 ◽  
Vol 10 (1) ◽  
pp. 37-46 ◽  
Author(s):  
D.I. Osman ◽  
L. Plöen
Keyword(s):  
Fine Structure ◽  
Sertoli Cells ◽  
Camelus Dromedarius

scholarly journals STUDIES ON THE FINE STRUCTURE OF THE MAMMALIAN TESTIS

1955 ◽  
Vol 1 (4) ◽  
pp. 287-300 ◽  
Author(s):  
Mario H. Burgos ◽  
Don W. Fawcett
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Golgi Complex ◽  
Nuclear Membrane ◽  
Posterior Margin ◽  
Frequent Occurrence ◽  
Thin Sections ◽  
Acrosomal Vesicle ◽  
Mammalian Testis

The differentiation of cat spermatids was studied in thin sections examined with the electron microscope. The Golgi complex of the spermatid consists of a central aggregation of minute vacuoles, partially surrounded by a lamellar arrangement of flattened vesicles. In the formation of the acrosome, one or more moderately dense homogeneous granules arise within vacuoles of the Golgi complex. The coalescence of these vacuoles and their contained granules gives rise to a single acrosomal granule within a sizable membrane-limited vacuole, termed the acrosomal vesicle. This adheres to the nuclear membrane and later becomes closely applied to the anterior two-thirds of the elongating nucleus to form a closed bilaminar head cap. The substance of the acrosomal granule occupies the narrow cleft between the membranous layers of the cap. The caudal sheath is comprised of many straight filaments extending backward from a ring which encircles the nucleus at the posterior margin of the head cap. Attention is directed to the frequent occurrence of pairs of spermatids joined by a protoplasmic bridge and the origin and possible significance of this relationship are discussed.

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