PERMANGANATE FIXATION OF THE GOLGI COMPLEX AND OTHER CYTOPLASMIC STRUCTURES OF MAMMALIAN TESTES

Hilton H. Mollenhauer; William Zebrun

doi:10.1083/jcb.8.3.761

PERMANGANATE FIXATION OF THE GOLGI COMPLEX AND OTHER CYTOPLASMIC STRUCTURES OF MAMMALIAN TESTES

The Journal of Cell Biology ◽

10.1083/jcb.8.3.761 ◽

1960 ◽

Vol 8 (3) ◽

pp. 761-775 ◽

Cited By ~ 34

Author(s):

Hilton H. Mollenhauer ◽

William Zebrun

Keyword(s):

Fine Structure ◽

Guinea Pig ◽

Golgi Complex ◽

Sertoli Cells ◽

Close Association ◽

High Electron ◽

Cell Organelles ◽

Different Dimensions ◽

Morphological Differences ◽

Cytoplasmic Structures

Observations on the fine structure of KMnO4-fixed testes of small mammals (guinea pig, rat, and mouse) reveal certain morphological differences between the spermatogenic and Sertoli cells which have not been demonstrated in the same tissue fixed with OsO4. Aggregates of minute circular profiles, much smaller than the spherical Golgi vesicles, are described in close association with the Golgi complex of developing spermatids. Groups of dense flattened vesicles, individually surrounded by a membrane of different dimensions than that which bounds most of the other cell organelles, appear dispersed within the cytoplasm of some spermatogenic cells. Flattened vesicles of greater density than those belonging to the Golgi complex are reported confined to the inner Golgi zone of developing guinea pig spermatids between the Golgi cisternae and the head cap. The profiles of endoplasmic reticulum within spermatocytes appear shorter, wider, and more tortuous than those of Sertoli cells. Minute cytoplasmic particles approximately 300 A in diameter and of high electron opacity appear randomly disposed in some Sertoli cells. Groups of irregular-shaped ovoid bodies within the developing spermatids are described as resembling portions of cytoplasm from closely adjacent spermatids. Interpretation is presented regarding the fine structure of KMnO4-fixed testes in view of what has already been reported for mammalian testes fixed in OsO4.

Download Full-text

The fine structure and phosphatase cytochemistry of the Golgi complex and associated structures in the Sertoli cells of Syrian hamsters

Cell and Tissue Research ◽

10.1007/bf00222068 ◽

1975 ◽

Vol 157 (2) ◽

Cited By ~ 5

Author(s):

I-Li Chen ◽

RobertD. Yates

Keyword(s):

Fine Structure ◽

Golgi Complex ◽

Sertoli Cells ◽

Syrian Hamsters ◽

Phosphatase Cytochemistry

Download Full-text

Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099532 ◽

1981 ◽

Vol 39 ◽

pp. 622-623

Author(s):

R. P. Becker ◽

J. J. Wolosewick ◽

J. Ross-Stanton

Keyword(s):

Fine Structure ◽

Tannic Acid ◽

Three Dimensional ◽

Albino Rats ◽

Cell Organelles ◽

Vascular Perfusion ◽

Thin Sections ◽

Carbon Coated ◽

Embedding Medium ◽

Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

Download Full-text

Respiratory Metabolism During Pregnancy in the Guinea Pig

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1957.190.3.425 ◽

1957 ◽

Vol 190 (3) ◽

pp. 425-428 ◽

Cited By ~ 2

Author(s):

Richard M. Hoar ◽

William C. Young

Keyword(s):

Heart Rate ◽

Oxygen Consumption ◽

Guinea Pig ◽

Metabolic Rate ◽

Adrenal Cortex ◽

Guinea Pigs ◽

Sharp Decrease ◽

Zona Fasciculata ◽

Respiratory Metabolism ◽

Morphological Differences

Oxygen consumption and heart rate during pregnancy were measured in untreated, thyroxin-injected and thyroidectomized guinea pigs given I131. From impregnation until parturition, oxygen consumption increased 7.9% in untreated females. The increase continued until 5 days postpartum when a sharp decrease occurred. The increase is not accounted for by growth of the fetal mass. Comparable increases occurred in thyroxin-injected (16.2%) and thyroidectomized (11.9%) females, although the levels throughout were higher and lower, respectively, than in intact females. Heart rate did not increase. On the contrary, statistically significant decreases occurred in the untreated and thyroxin-injected females. Although the mechanism associated with the increased metabolic rate is not known, the possibility of thyroid participation would seem to be excluded. Involvement of the adrenal cortex is suggested by morphological differences in the cells of the zona fasciculata in pregnant and nonpregnant females and by evidence cited from other studies.

Download Full-text

Observations on the fine structure of the baroreceptors and adrenergic innervation of the guinea-pig carotid sinus

Journal of Morphology ◽

10.1002/jmor.1051940106 ◽

1987 ◽

Vol 194 (1) ◽

pp. 65-74 ◽

Cited By ~ 5

Author(s):

H. S. Shin ◽

W. C. Hulbert ◽

D. F. Biggs

Keyword(s):

Fine Structure ◽

Guinea Pig ◽

Carotid Sinus ◽

Adrenergic Innervation

Download Full-text

EXTIRPATION OF THE THYMUS IN THE GUINEA PIG

Journal of Experimental Medicine ◽

10.1084/jem.25.1.129 ◽

1917 ◽

Vol 25 (1) ◽

pp. 129-152 ◽

Cited By ~ 15

Author(s):

Edwards A. Park

Keyword(s):

Guinea Pig ◽

Serial Section ◽

Guinea Pigs ◽

Close Association ◽

Main Lobe ◽

Technical Error ◽

Serial Sections ◽

Pharyngeal Pouch ◽

The Third ◽

Third Pharyngeal Pouch

1. Accessory lobes of thymus, derived from the third pharyngeal pouch, occurring in close association with the parathyroids from the third pouch, were found in serial section of the cervical tissues of eleven out of fourteen guinea pigs, and probably would have been found in all fourteen but for a technical error. 2. It is probable, therefore, that accessory lobes of thymus having this situation and origin are usually, if not always, present in the guinea pig. 3. Additional accessory lobes of thymus belonging to, but at some distance from the main lobe were also present in several of the animals. 4. The discovery of these accessory lobes makes it certain that the guinea pig is unsuitable material for complete thymectomy, and probably complete extirpation of the thymus in this animal is rarely, if ever accomplished. 5. The extirpation experiments of previous investigators in the guinea pig must now be regarded as partial extirpations, and their results interpreted in that light. 6. Extirpation of the thymus in the guinea pig produced no changes in the writer's experiments. 7. The study of the serial sections of the cervical tissues of the guinea pig indicates that Ruben's statements regarding the parathyroid derived from the fourth pharyngeal pouch in the guinea pig are correct,—that it is much smaller than parathyroid III, may be rudimentary, and is sometimes absent at least on one side. 8. No accessory lobe of thymus was found accompanying the parathyroid from the fourth pouch, a finding also bearing out Ruben's statement that no thymus anlarge springs from the fourth pouch in the guinea pig.

Download Full-text