Three-dimensional development of luminal projections and junctional complexes in the developing central nervous system blood vessels of the rat

ABSTRACT Recent advances of three-dimensional (3D) Doppler application is HDlive flow providing a realistic rendering of fine peripheral blood vessels, such as vascularity of the lung, brain, and eyeballs. The picture of the month demonstrates an oblique– sagittal view of the brain vascularity. Flow imaging can add both angiostructural and functional information to structural findings of normal and abnormal central nervous system. How to cite this article Pooh RK. 20-week Brain Vascularity by Transvaginal 3D HDlive Flow. Donald School J Ultrasound Obstet Gynecol 2016;10(3):203-204.

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Confocal laser microscopy of impregnated central nervous system tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102559 ◽

1988 ◽

Vol 46 ◽

pp. 94-95

Author(s):

J.N. Turner ◽

M. Siemens ◽

D. Szarowski ◽

D.N. Collins

Keyword(s):

Electron Microscopy ◽

Central Nervous System ◽

Nervous System ◽

Three Dimensional ◽

Dimensional Structure ◽

Confocal Laser ◽

High Contrast ◽

Central Nervous System Tissue ◽

Tissue Samples ◽

Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

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Cytochemical Specializations on the Luminal Surface of Blood Vessels in the Developing Rat Central Nervous System

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115936 ◽

1975 ◽

Vol 33 ◽

pp. 462-463

Author(s):

R. S. Hannah ◽

T. H. Rosenquist

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Blood Vessels ◽

Alcian Blue ◽

Luminal Surface ◽

High Voltage Electron Microscopy ◽

Voltage Electron ◽

Concentration Method ◽

Endothelial Surface ◽

Cacodylate Buffer

Developing blood vessels in the rat central nervous system exhibit several unusual luminal features. Hannah (1975) used high voltage electron microscopy to demonstrate numerous ridges of endothelium, some near junctional complexes. The ridges produced troughs (which may appear as depressions) in the endothelial surface. In some areas ridges extended over the troughs, removing them from direct contact with the luminal surface. At no time were the troughs observed to penetrate the basal laminae. Fingerlike projections also extended into the lumina.To determine whether any chemical specializations accompanied the unusual morphological features of the luminal surface, we added 0.1% Alcian blue (Behnke and Zelander, 1970) to the 3% glutaraldehyde perfusate (cacodylate buffer, pH 7.4). After Alcian blue had reacted with the luminal glycocalyces, the dye was dissociated with MgCl2 via critical electrolyte concentration method of Scott and Dorling (1965). When these methods are applied together, it is possible to differentiate mucopolysaccharides (glycosaminoglycans or GAG) with the electron microscope.

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