Identification of Haynaldia villosa chromosomes added to wheat using a sequential C-banding and genomic in situ hybridization technique

1996 ◽  
Vol 92 (1) ◽  
pp. 116-120 ◽  
Author(s):  
S. B. Zhong ◽  
D. Y. Zhang ◽  
H. B. Li ◽  
J. X. Yao
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Hybridization Technique ◽  
Haynaldia Villosa ◽  
C Banding

Identification of the parental chromosomes of the wheat–alien amphiploid Agrotana by genomic in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1163-1169 ◽  
Author(s):  
Qin Chen ◽  
R. L. Conner ◽  
A. Laroche
Keyword(s):  
In Situ Hybridization ◽  
Genomic Dna ◽  
Molecular Cytogenetics ◽  
Unknown Origin ◽  
Genomic In Situ Hybridization ◽  
Haynaldia Villosa ◽  
Genomic Relationships ◽  
High Degree ◽  
Donor Species

Labelled total genomic DNA from four alien species, Thinopyrum ponticum (Host) Beauv. (2n = 70, genomes J1J1J1J2J2), Th. bessarabicum (Savul. &Rayss) Love (2n = 14, genome J), Th. elongatum (Host) Beauv. (2n = 14, genome E), and Haynaldia villosa (L.) Schur. (2n = 14, genome V), were used as probes in combination with blocking wheat DNA for in situ hybridization of the chromosomes of Agrotana, a wheat–alien hybrid (2n = 56) of unknown origin. The results showed that genomic DNA probes from Th. ponticum and Th. bessarabicum both clearly revealed 16 alien and 40 wheat chromosomes in Agrotana, indicating that the J genome present in these two species has a high degree of homology with the alien chromosomes in Agrotana. Biotinylated genomic DNA probe from Th. elongatum identified 10 chromosomes from Agrotana, while some regions of six other chromosomes yielded a weak or no signal. The probe from H. villosa produced no differential labelling of the chromosomes of Agrotana. The genomic formula of Agrotana was designated as AABBDDJJ. We suggest that the alien parent donor species of Agrotana is Th. ponticum rather than Th. bessarabicum. Genomic relationships of the three Thinopyrum species are discussed in relation to the distribution of GISH signals in the chromosomes of Agrotana.Key words: Thinopyrum species, wheat–alien amphiploid, genomic DNA probing, in situ hybridization, molecular cytogenetics.

Meiotic pairing in wheat-rye derivatives detected by genomic in situ hybridization and C-banding ? A comparative analysis

Chromosoma ◽  
1995 ◽  
Vol 103 (8) ◽  
pp. 554-558 ◽  
Author(s):  
Bego�a Fern�ndez-Calv�n ◽  
Elena Benavente ◽  
Juan Orellana
Keyword(s):  
In Situ Hybridization ◽  
Comparative Analysis ◽  
Genomic In Situ Hybridization ◽  
Meiotic Pairing ◽  
C Banding

Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena)

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 613-618 ◽  
Author(s):  
E. N. Jellen ◽  
B. S. Gill ◽  
T. S. Cox
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genomic In Situ Hybridization ◽  
Hybridization Pattern ◽  
C Banding ◽  
Intergenomic Translocations ◽  
C Genome ◽  
Total Dna

The genomic in situ hybridization (GISH) technique was used to discriminate between chromosomes of the C genome and those of the A and A/D genomes in allopolyploid oat species (genus Avena). Total biotinylated DNA from A. strigosa (2n = 2x = 14, AsAs genome) was mixed with sheared, unlabelled total DNA from A. eriantha (2n = 2x = 14, CpCp) at a ratio of 1:200 (labelled to unlabelled). The resulting hybridization pattern consisted of 28 mostly labelled and 14 mostly unlabelled chromosomes in the hexaploids. Attempts to discriminate between chromosomes of the A and D genomes in A. sativa (2n = 6x = 42, AACCDD) were unsuccessful using GISH. At least eight intergenomic translocation segments were detected in A. sativa 'Ogle', several of which were not observed in A. byzantina 'Kanota' (2n = 6x = 42, AACCDD) or in A. sterilis CW 439-2 (2n = 6x = 42, AACCDD). At least five intergenomic translocation segments were observed in A. maroccana CI 8330 'Magna' (2n = 4x = 28, AACC). In both 'Ogle' and 'Magna', positions of most of these translocations matched with C-banding patterns.Key words: Avena sativa, oat, in situ hybridization, C-banding, Avena macrostachya.

Sequential chromosome banding and in situ hybridization analysis

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 792-795 ◽  
Author(s):  
Jiming Jiang ◽  
Bikram S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Acid Treatment ◽  
Chromosome Banding ◽  
Molecular Mapping ◽  
Genome Mapping ◽  
Genomic In Situ Hybridization ◽  
Metaphase Chromosomes ◽  
C Banding

Different combinations of chromosome N- or C-banding with in situ hybridization (ISH) or genomic in situ hybridization (GISH) were sequentially performed on metaphase chromosomes of wheat. A modified N-banding–ISH/GISH sequential procedure gave best results. Similarly, a modified C-banding – ISH/GISH procedure also gave satisfactory results. The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH. By the sequential chromosome banding – ISH/GISH analysis, multicopy DNA sequences and the breakpoints of wheat–alien translocations were directly allocated to specific chromosomes of wheat. The sequential chromosome banding– ISH/GISH technique should be widely applicable in genome mapping, especially in cytogenetic and molecular mapping of heterochromatic and euchromatic regions of plant and animal chromosomes.Key words: N-banding, C-banding, in situ hybridization, genomic in situ hybridization.

Meiotic pairing in wheat-rye derivatives detected by genomic in situ hybridization and C-banding ? A comparative analysis

Chromosoma ◽  
1995 ◽  
Vol 103 (8) ◽  
pp. 554-558
Author(s):  
Bego�a Fern�ndez-Calv�n ◽  
Elena Benavente ◽  
Juan Orellana
Keyword(s):  
In Situ Hybridization ◽  
Comparative Analysis ◽  
Genomic In Situ Hybridization ◽  
Meiotic Pairing ◽  
C Banding

Molecular cytogenetic analysis of Agropyron chromatin specifying resistance to barley yellow dwarf virus in wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 336-347 ◽  
Author(s):  
Uwe Hohmann ◽  
Winfried Busch ◽  
Katia Badaeva ◽  
Bernd Friebe ◽  
Bikram S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Barley Yellow Dwarf Virus ◽  
Genomic In Situ Hybridization ◽  
Yellow Dwarf ◽  
Deletion Mapping ◽  
Addition Line ◽  
C Banding ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Nine families of bread wheat (TC5, TC6, TC7, TC8, TC9, TC10, TC14, 5395-(243AA), and 5395) with resistance to barley yellow dwarf virus and containing putative translocations between wheat and a group 7 chromosome of Agropyron intermedium (L1 disomic addition line, 7Ai#1 chromosome) induced by homoeologous pairing or tissue culture were analyzed. C-banding, genomic in situ hybridization (GISH), and restriction fragment length polymorphism (RFLP) in combination with repetitive Agropyron-specific sequences and deletion mapping in wheat were used to determine the relative locations of the translocation breakpoints and the size of the transferred alien chromatin segments in hexaploid wheat–Agropyron translocation lines. All homoeologous compensating lines had complete 7Ai#1 or translocated 7Ai#1–7D chromosomes that substitute for chromosome 7D. Two complete 7Ai#1 (7D) substitution lines (5395-(243AA) and 5395), one T1BS–7Ai#1S∙7Ai#1L addition line (TC7), and two different translocation types, T7DS–7Ai#1S∙7Ai#1L (TC5, TC6, TC8, TC9, and TC10) and T7DS∙7DL–7Ai#1L (TC14), substituting for chromosome 7D were identified. The substitution line 5395-(243AA) had a reciprocal T1BS∙1BL–4BS/T1BL–4BS∙4BL translocation. TC14 has a 6G (6B) substitution. The RFLP data from deletion mapping studies in wheat using 37 group 7 clones provided 10 molecular tagged chromosome regions for homoeologous and syntenic group 7 wheat or Agropyron chromosomes. Together with GISH we identified three different sizes of the transferred Agropyron chromosome segments with approximate breakpoints at fraction length (FL) 0.33 in the short arm of chromosome T7DS–7Ai#1S∙7Ai#1L (TC5, TC6, TC8, TC9, and TC10) and another at FL 0.37 of the nonhomoeologous translocated chromosome T1BS–7Ai#1S∙7Ai#1L (TC7). One breakpoint was identified in the long arm of chromosome T7DS∙7DL–7Ai#1L (TC14) at FL 0.56. We detected some nonreciprocal translocations for the most proximal region of the chromosome arm of 7DL, which resulted in small duplications. Key words : C-banding, genomic in situ hybridization (GISH), physical mapping, translocation mapping, RFLP analysis.

Characterization of Pacific abalone(Haliotis discus hannai)karyotype by C-banding and fluorescence in situ hybridization with rDNA

2013 ◽  
Vol 37 (7) ◽  
pp. 1002 ◽  
Author(s):  
Mingyi CAI ◽  
Xiande LIU ◽  
Ziying CHEN ◽  
Bingbing CAI ◽  
Caihuan KE
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Haliotis Discus Hannai ◽  
Pacific Abalone ◽  
C Banding ◽  
Haliotis Discus ◽  
Abalone Haliotis Discus

C-banding and fluorescence in situ hybridization studies of the wheat–alien hybrid 'Agrotana'

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 477-481 ◽  
Author(s):  
Jie Xu ◽  
R. L. Conner ◽  
A. Laroche
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic Dna ◽  
Chromosome Engineering ◽  
C Banding ◽  
Chinese Spring Wheat ◽  
Mother Cells ◽  
Alien Chromatin ◽  
Chromosome Segments

'Agrotana', a wheat-alien hybrid (2n = 56), is a potential source of resistance to common root rot, stem rust, wheat streak mosaic virus, and the wheat curl mite. However, the origin of 'Agrotana', reported to be durum wheat × Agropyron trichophorum (pubescent wheatgrass), is uncertain. The objective of this investigation was to determine the chromosome constitution of 'Agrotana' using C-banding and fluorescence in situ hybridization techniques. The F1 hybrid of 'Agrotana' × 'Chinese Spring' wheat showed 7 I + 21 II in 14.9% of the pollen mother cells, evidence of the presence of the A, B, and D genomes in 'Agrotana'. The hybrid had 16 heavily C-banded chromosomes, namely 4A, and 1-7B of wheat, and a translocation that probably involved wheat chromosomes 2A and 2D. In situ hybridization using biotinylated genomic DNA of Ag. trichophorum cv. Greenleaf blocked with CS DNA failed to identify the alien chromosomes in 'Agrotana', indicating that the alien chromosomes were not likely derived from pubescent wheatgrass. In situ hybridization using labelled wheat genomic DNA blocked with 'Agrotana' DNA revealed that 'Agrotana' had 40 wheat, 14 alien, and 2 (a pair) wheat–alien translocated chromosomes. There was no homology between wheat and the alien chromosomes or chromosome segments involved in the wheat–alien recombinant. Two of the seven pairs of alien chromosomes were homoeologous to each other. The ability to identify alien chromatin in wheat using labelled wheat DNA instead of labelled alien DNA will be particularly useful in chromosome engineering of wheat germplasms having alien chromatin of unknown origin.Key words: wheat–alien hybrid, C-banding, fluorescence in situ hybridization, labelled wheat DNA as probe.

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