Meiotic pairing in wheat-rye derivatives detected by genomic in situ hybridization and C-banding ? A comparative analysis

Chromosoma ◽  
1995 ◽  
Vol 103 (8) ◽  
pp. 554-558 ◽  
Author(s):  
Bego�a Fern�ndez-Calv�n ◽  
Elena Benavente ◽  
Juan Orellana
Keyword(s):  
In Situ Hybridization ◽  
Comparative Analysis ◽  
Genomic In Situ Hybridization ◽  
Meiotic Pairing ◽  
C Banding

Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena)

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 613-618 ◽  
Author(s):  
E. N. Jellen ◽  
B. S. Gill ◽  
T. S. Cox
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genomic In Situ Hybridization ◽  
Hybridization Pattern ◽  
C Banding ◽  
Intergenomic Translocations ◽  
C Genome ◽  
Total Dna

The genomic in situ hybridization (GISH) technique was used to discriminate between chromosomes of the C genome and those of the A and A/D genomes in allopolyploid oat species (genus Avena). Total biotinylated DNA from A. strigosa (2n = 2x = 14, AsAs genome) was mixed with sheared, unlabelled total DNA from A. eriantha (2n = 2x = 14, CpCp) at a ratio of 1:200 (labelled to unlabelled). The resulting hybridization pattern consisted of 28 mostly labelled and 14 mostly unlabelled chromosomes in the hexaploids. Attempts to discriminate between chromosomes of the A and D genomes in A. sativa (2n = 6x = 42, AACCDD) were unsuccessful using GISH. At least eight intergenomic translocation segments were detected in A. sativa 'Ogle', several of which were not observed in A. byzantina 'Kanota' (2n = 6x = 42, AACCDD) or in A. sterilis CW 439-2 (2n = 6x = 42, AACCDD). At least five intergenomic translocation segments were observed in A. maroccana CI 8330 'Magna' (2n = 4x = 28, AACC). In both 'Ogle' and 'Magna', positions of most of these translocations matched with C-banding patterns.Key words: Avena sativa, oat, in situ hybridization, C-banding, Avena macrostachya.

Sequential chromosome banding and in situ hybridization analysis

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 792-795 ◽  
Author(s):  
Jiming Jiang ◽  
Bikram S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Acid Treatment ◽  
Chromosome Banding ◽  
Molecular Mapping ◽  
Genome Mapping ◽  
Genomic In Situ Hybridization ◽  
Metaphase Chromosomes ◽  
C Banding

Different combinations of chromosome N- or C-banding with in situ hybridization (ISH) or genomic in situ hybridization (GISH) were sequentially performed on metaphase chromosomes of wheat. A modified N-banding–ISH/GISH sequential procedure gave best results. Similarly, a modified C-banding – ISH/GISH procedure also gave satisfactory results. The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH. By the sequential chromosome banding – ISH/GISH analysis, multicopy DNA sequences and the breakpoints of wheat–alien translocations were directly allocated to specific chromosomes of wheat. The sequential chromosome banding– ISH/GISH technique should be widely applicable in genome mapping, especially in cytogenetic and molecular mapping of heterochromatic and euchromatic regions of plant and animal chromosomes.Key words: N-banding, C-banding, in situ hybridization, genomic in situ hybridization.

Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 712-719 ◽  
Author(s):  
George Fedak ◽  
Qin Chen ◽  
Robert L Conner ◽  
André Laroche ◽  
René Petroski ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
The Other ◽  
Meiotic Pairing ◽  
Genomic Constitution ◽  
Meiotic Analysis ◽  
Partial Amphiploid ◽  
Partial Amphiploids

A combination of genomic in situ hybridization (GISH) and meiotic pairing analysis of wheat-Thinopyrum partial amphiploids was employed to identify the genomic constitution and relationships between partial amphiploids derived from wheat and wheatgrass crosses. On the basis of similarities in the meiotic behavior and GISH patterns, the alien chromosomes of two of eight partial amphiploids, TAF46 and 'Otrastayuskaya 38', were judged to originate from Th. intermedium, whereas Th. ponticum was one of the parents of the other six partial amphiploids; PWM706, PWM206, PWM209, PWMIII, OK7211542, and Ag-wheat hybrid. Each of these partial amphiploids was found to contain a synthetic alien genome composed of different combinations of St-, J-, or Js-genome chromosomes. For relatedness of partial amphiploid lines, meiotic analysis of F1 hybrids and GISH results were generally complementary, but the latter offered greater precision in identifying constituent genomes.Key words: wheat, Thinopyrum, partial amphiploids, GISH, meiotic analysis.

Molecular cytogenetic analysis of Agropyron chromatin specifying resistance to barley yellow dwarf virus in wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 336-347 ◽  
Author(s):  
Uwe Hohmann ◽  
Winfried Busch ◽  
Katia Badaeva ◽  
Bernd Friebe ◽  
Bikram S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Barley Yellow Dwarf Virus ◽  
Genomic In Situ Hybridization ◽  
Yellow Dwarf ◽  
Deletion Mapping ◽  
Addition Line ◽  
C Banding ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Nine families of bread wheat (TC5, TC6, TC7, TC8, TC9, TC10, TC14, 5395-(243AA), and 5395) with resistance to barley yellow dwarf virus and containing putative translocations between wheat and a group 7 chromosome of Agropyron intermedium (L1 disomic addition line, 7Ai#1 chromosome) induced by homoeologous pairing or tissue culture were analyzed. C-banding, genomic in situ hybridization (GISH), and restriction fragment length polymorphism (RFLP) in combination with repetitive Agropyron-specific sequences and deletion mapping in wheat were used to determine the relative locations of the translocation breakpoints and the size of the transferred alien chromatin segments in hexaploid wheat–Agropyron translocation lines. All homoeologous compensating lines had complete 7Ai#1 or translocated 7Ai#1–7D chromosomes that substitute for chromosome 7D. Two complete 7Ai#1 (7D) substitution lines (5395-(243AA) and 5395), one T1BS–7Ai#1S∙7Ai#1L addition line (TC7), and two different translocation types, T7DS–7Ai#1S∙7Ai#1L (TC5, TC6, TC8, TC9, and TC10) and T7DS∙7DL–7Ai#1L (TC14), substituting for chromosome 7D were identified. The substitution line 5395-(243AA) had a reciprocal T1BS∙1BL–4BS/T1BL–4BS∙4BL translocation. TC14 has a 6G (6B) substitution. The RFLP data from deletion mapping studies in wheat using 37 group 7 clones provided 10 molecular tagged chromosome regions for homoeologous and syntenic group 7 wheat or Agropyron chromosomes. Together with GISH we identified three different sizes of the transferred Agropyron chromosome segments with approximate breakpoints at fraction length (FL) 0.33 in the short arm of chromosome T7DS–7Ai#1S∙7Ai#1L (TC5, TC6, TC8, TC9, and TC10) and another at FL 0.37 of the nonhomoeologous translocated chromosome T1BS–7Ai#1S∙7Ai#1L (TC7). One breakpoint was identified in the long arm of chromosome T7DS∙7DL–7Ai#1L (TC14) at FL 0.56. We detected some nonreciprocal translocations for the most proximal region of the chromosome arm of 7DL, which resulted in small duplications. Key words : C-banding, genomic in situ hybridization (GISH), physical mapping, translocation mapping, RFLP analysis.

Sign in / Sign up

Export Citation Format

Share Document