Effects of Ca2+ on the salt-stress response of barley roots as observed by in-vivo 31P-nuclear magnetic resonance and in-vitro analysis

Planta ◽  
1993 ◽  
Vol 190 (4) ◽  
Author(s):  
Vicente Martinez ◽  
Andr� L�uchli
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Salt Stress ◽  
Stress Response ◽  
Magnetic Resonance ◽  
Salt Stress Response ◽  
31P Nuclear Magnetic Resonance ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals In vitro analysis of elongation and termination by mutant RNA polymerases with altered termination behavior.

1996 ◽  
Vol 16 (11) ◽  
pp. 6468-6476 ◽  
Author(s):  
S A Shaaban ◽  
E V Bobkova ◽  
D M Chudzik ◽  
B D Hall
Keyword(s):  
Rna Polymerase Iii ◽  
Multiple Roles ◽  
Wild Type ◽  
Protein Motifs ◽  
Study Support ◽  
Vitro Analysis ◽  
Pol Iii ◽  
In Vitro Analysis

We have studied the in vitro elongation and termination properties of several yeast RNA polymerase III (pol III) mutant enzymes that have altered in vivo termination behavior (S. A. Shaaban, B. M. Krupp, and B. D. Hall, Mol. Cell. Biol. 15:1467-1478, 1995). The pattern of completed-transcript release was also characterized for three of the mutant enzymes. The mutations studied occupy amino acid regions 300 to 325, 455 to 521, and 1061 to 1082 of the RET1 protein (P. James, S. Whelen, and B. D. Hall, J. Biol. Chem. 266:5616-5624, 1991), the second largest subunit of yeast RNA pol III. In general, mutant enzymes which have increased termination require a longer time to traverse a template gene than does wild-type pol III; the converse holds true for most decreased-termination mutants. One increased-termination mutant (K310T I324K) was faster and two reduced termination mutants (K512N and T455I E478K) were slower than the wild-type enzyme. In most cases, these changes in overall elongation kinetics can be accounted for by a correspondingly longer or shorter dwell time at pause sites within the SUP4 tRNA(Tyr) gene. Of the three mutants analyzed for RNA release, one (T455I) was similar to the wild type while the two others (T455I E478K and E478K) bound the completed SUP4 pre-tRNA more avidly. The results of this study support the view that termination is a multistep pathway in which several different regions of the RET1 protein are actively involved. Region 300 to 325 likely affects a step involved in RNA release, while the Rif homology region, amino acids 455 to 521, interacts with the nascent RNA 3' end. The dual effects of several mutations on both elongation kinetics and RNA release suggest that the protein motifs affected by them have multiple roles in the steps leading to transcription termination.

Multifactorial Regulation of Cardiac Gene Expression: an In Vivo and In Vitro Analysis

1995 ◽  
Vol 752 (1 Cardiac Growt) ◽  
pp. 370-386 ◽  
Author(s):  
J. L. SAMUEL ◽  
I. DUBUS ◽  
F. FARHADIAN ◽  
F. MAROTTE ◽  
P. OLIVIERO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cardiac Gene Expression ◽  
Cardiac Gene ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Cirrhosis of the human liver: an in vitro 31P nuclear magnetic resonance study

1995 ◽  
Vol 1272 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Simon D. Taylor-Robinson ◽  
E.Louise Thomas ◽  
Janet Sargentoni ◽  
Claude D. Marcus ◽  
Brian R. Davidson ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Human Liver ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
31P Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

scholarly journals Risedronate Effects on the In Vivo Bioactive Glass Behavior: Nuclear Magnetic Resonance and Histopathological Studies

2019 ◽  
Vol 2019 ◽  
pp. 1-16
Author(s):  
Siwar Mosbahi ◽  
Hassane Oudadesse ◽  
Claire Roiland ◽  
Bertrand Lefeuvre ◽  
Lotfi Slimani ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Bioactive Glass ◽  
Volume Fraction ◽  
Chemical Reactivity ◽  
Femoral Condyle ◽  
Mineral Density ◽  
Nuclear Magnetic

The present study aimed to enhance the anti-osteoporotic performance of bioactive glass (46S6) through its association with bisphosphonate such as risedronate with amounts of 8, 12, and 20%. Obtained composites have been called 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS, respectively. In vitro and in vivo explorations have been carried out. Bioactive glass and risedronate association has been performed by adsorption process. Structure analyses have been carried out to evaluate and to understand their chemical interactions. Solid Nuclear Magnetic Resonance (NMR) has been employed to study the structural properties of obtained biocomposite. The spectra deconvolution showed the appearance of a species (Q4) in the biocomposites 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS indicating their successful chemical association. In vitro experiments showed the enhancement of the chemical reactivity of the composites 46S6-xRIS compared to the pure bioactive glass. In fact, the silicon liberation after 30 days of immersion was 50 ppm for pure bioactive glass 46S6, and 41, 64, and 62 from 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS, respectively. Based on the in vitro results, 46S6-8RIS was implanted in the femoral condyle of an ovariectomized rat and compared with implanted pure glass in the goal to highlight its anti-osteoporotic performance. After 60 days, implanted group with 46S6-8RIS showed the increase in bone mineral density (BMD with 10%) and bone volume fraction (BV/TV with 80%) and the decrease in trabecular separation (Tb/Sp with 74%) when compared to that of 46S6 group. These results are confirmed by the histopathological analyses, which showed the bone trabeculae reconnection after the 46S6-8RIS implantation. Chemical analyses showed the reduction in silicon (Si) and sodium (Na) ion concentrations, and the rise in calcium (Ca) and phosphorus (P) ion levels, which was explained by the dissolution of biocomposite matrix and the deposition of hydroxyapatite layer. Histomorphometric results highlighted the risedronate effect on the antiosteoporotic phenomenon. Obtained results showed good behavior with only 8% of introduced risedronate in the glass matrix.

Work in Progress: In Vitro Analysis of Pleural Fluid Analogs by Proton Magnetic Resonance Preliminary Studies at 1.5T

1987 ◽  
Vol 22 (5) ◽  
pp. 382-387 ◽  
Author(s):  
PETER VOCK ◽  
LAURENCE W. HEDLUND ◽  
ROBERT J. HERFKENS ◽  
ERIC L. EFFMANN ◽  
MARK A. BROWN ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Pleural Fluid ◽  
Proton Magnetic Resonance ◽  
Work In Progress ◽  
Vitro Analysis ◽  
In Vitro Analysis
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