Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. and the wild species S. circaeifolium subsp. circaeifolium Bitter exhibiting resistance to Phytophthora infestans (Mont.) de Bary and Globodera pallida (Stone) Behrens

1992 ◽  
Vol 84-84 (3-4) ◽  
pp. 362-370 ◽  
Author(s):  
K. M. Louwes ◽  
R. Hoekstra ◽  
W. M. Mattheij
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Interspecific Hybridization ◽  
Wild Species ◽  
Globodera Pallida ◽  
Cultivated Potato

Foliar and tuber assessment of late blight (Phytophthora infestans (Mont.) de Bary) reaction in cultivated potato (Solanum tuberosum L.)

2002 ◽  
Vol 45 (2-4) ◽  
pp. 215-224 ◽  
Author(s):  
D. S. Douches ◽  
W. W. Kirk ◽  
M. A. Bertram ◽  
J. J. Coombs ◽  
B. A. Niemira
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Cultivated Potato

scholarly journals Asociaciones de marcadores moleculares con la resistencia a enfermedades, caracteres morfológicos y agronómicos en familias diploides de papa (Solanum tuberosum L.)

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Julio Gabriel ◽  
Silene Veramendi ◽  
Linett Pinto ◽  
Leslie Pariente ◽  
Ada Angulo
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Globodera Pallida ◽  
Solanum Stenotomum ◽  
Synchytrium Endobioticum ◽  
Para Determinar

<p>Quince familias de papa (840 genotipos) provenientes de cruzas inter-específicas entre especies de Solanum stenotomum, S. goniocalyx y S. phureja fueron genotipadas, con el objetivo de asociar  seis marcadores moleculares (GP94, HC, Nl25, Gro 1-4, RYSC3 y CP60) con genes mayores de resistencia para tizón tardío (Phytophthora infestans), verruga (Synchytrium endobioticum), nematodo - quiste (Globodera pallida y G. rostochiensis) y virus PVY y PVX. Los resultados mostraron que cinco de los marcadores aplicados fueron polimórficos y amplificaron en más del 80% de las familias. El marcador RYSC3 que co-localiza con el gen Ry no amplificó en ninguna de las familias evaluadas. La familia 8 amplificó la banda para tres marcadores (CP60, GP94 y NL25) en la totalidad de sus clones. La prueba de χsup2 se utilizó para determinar el ajuste de las proporciones de segregación de cada familia para cada marcador y genotipar los progenitores. Nueve caracteres agronómicos y morfológicos fueron evaluados en la cosecha. Mediante agrupamiento cluster fueron seleccionados 107 clones con resistencia a PVX, P. infestans, G. rostochiensis y S. endobioticum, alto rendimiento y volumen de tubérculos, elevado número de tubérculos y ojos superficiales. Sobre la base de estos resultados, aspectos prácticos para la aplicación eficiente de la selección asistida por marcadores moleculares son discutidos en este artículo.</p>

scholarly journals Short communication. Introgression of late blight (Phytophthora infestans L.) resistance from tuber-bearing Solanum wild species into cultivated potato

2011 ◽  
Vol 9 (1) ◽  
pp. 193 ◽  
Author(s):  
J. Gabriel ◽  
J. I. Ruiz de Galarreta ◽  
R. Lopez-Pardo ◽  
L. Barandalla ◽  
C. Alvarado ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Wild Species ◽  
Short Communication ◽  
Cultivated Potato

scholarly journals Tight Physical Linkage of the Nematode Resistance Gene Gpa2 and the Virus Resistance Gene Rx on a Single Segment Introgressed from the Wild Species Solanum tuberosum subsp. andigena CPC 1673 into Cultivated Potato

1999 ◽  
Vol 12 (3) ◽  
pp. 197-206 ◽  
Author(s):  
Jeroen Rouppe van der Voort ◽  
Konstantin Kanyuka ◽  
Edwin van der Vossen ◽  
Abdelhafid Bendahmane ◽  
Paul Mooijman ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Resistance Gene ◽  
Potato Virus ◽  
Wild Species ◽  
Potato Virus X ◽  
Globodera Pallida ◽  
Cyst Nematodes ◽  
Total Size ◽  
Bac Clones ◽  
Mapping Populations

Comparative mapping studies within several plant genera have shown that genes for resistance to taxonomically diverse pathogens are often co-localized on the maps of these genotypes. Here we describe the genetic analysis of such a gene cluster on chromosome 12 of potato. This cluster harbors the resistance loci Gpa2 and Rx, which confer resistance to the potato cyst nematode Globodera pallida and potato virus X (PVX), respectively. By screening two mapping populations with 10 CAPS (cleaved amplified polymorphic sequence) markers derived from AFLP (amplified fragment length polymorphism) markers and the ends of overlapping bacterial artificial chromosome (BAC) clones, it is demonstrated that Gpa2 and Rx are tightly linked. From a recombination fraction of 1/3386, found after screening two different mapping populations, a genetic distance of 0.02 cM between Gpa2 and Rx was calculated. The physical length of this genetic interval does not exceed 300 kb of DNA as determined by the total size of overlapping BAC clones in the Rx/Gpa2 contig. Evidence is provided that both genes have been introgressed from a single wild species clone, Solanum tuberosum subsp. andigena CPC 1673. This suggests that these genes underlying the disease resistance specificities to potato virus X and potato cyst nematodes have a common evolutionary origin.

scholarly journals Marcadores moleculares asociados a genes/QTLs de resistencia para factores bióticos en nuevas variedades de papa (Solanum tuberosum L.) de Bolivia

2016 ◽  
Vol 16 (2) ◽  
pp. 209-232
Author(s):  
S. Veramendi ◽  
M. Baldelomar ◽  
A. Terán ◽  
J. Gabriel
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Globodera Pallida

Con el objetivo de asociar genes/QTLs de resistencia a los virus PVY, PVX, a los nematodos (Globodera pallida y G. rostochiensis), al tizón (Phytophthora infestans) y a la verruga (Synchytrium endobóticum) en 20 variedades de papa, incluida la variedad Waych'a como control susceptible, se evaluaron siete marcadores moleculares para SCAR, CAPS y PCR. Los resultados mostraron que el alelo del marcador RySC3 co-localizado con el gen Ryadg de resistencia a PVY, fue observado en todas las variedades evaluadas. El alelo para el marcador CP60 co-localizado con el gen Rx1 para resistencia a PVX, fue observado en todas las variedades excepto en Chota Ñawi. El alelo para el marcador HC co-localizado con el gen/QTL RGp5-vrnHC para resistencia a G. pallida, fue observado en las variedades Aurora, Chota Ñawi, Isabel, Keila, Morita, P'alta Chola, Pujyuni imilla, Robusta, Rosada, Victoria, Violeta, Yungueñita, Jaspe e India. El alelo para el marcador Gro1-4 co-localizado con el gen Gro1-4 para resistencia a G. rostochiensis, fue observado en las variedades Chota Ñawi, Isabel, Keila, Victoria, Violeta, Jaspe e India. El alelo para el marcador GP94 co-localizado con el gen/QTL Rpi-phu 1 para resistencia a P. infestans, fue observado en todas las variedades. Finalmente el alelo para el marcador NL25 co-localizado con el gen Sen1 para resistencia a S. endobioticum, fue también observado en todas las variedades.Aceptado para publicación: Noviembre 30, 2011

Разработка и тестирование универсальных праймеров для ПЦР-амплификации генов-ортологов β-фруктофуранозидазы (Pain-1) у видов и сортов картофеля

2018 ◽  
Author(s):  
E.O. Shmelkova ◽  
M.A. Slugina ◽  
A.A. Meleshin ◽  
E.V. Romanova
Keyword(s):  
Solanum Tuberosum ◽  
Wild Species ◽  
Starch Synthesis ◽  
Full Length ◽  
Universal Primers ◽  
Potato Tubers ◽  
Vacuolar Invertase ◽  
Reserve Carbohydrate ◽  
Cold Induced Sweetening ◽  
Design And Testing

Работа посвящена разработке и тестированию универсальных праймеров для ПЦР-амплификации полноразмерных генов-ортологов β-фруктофуранозидазы (кислой вакуолярной инвертазы) у видов и сортов картофеля (Solanum tuberosum). Крахмал – основной источник энергии и резервный углевод, накапливающийся в амилопластах клубней. Образовавшаяся в результате фотосинтеза молекула глюкозы при реакции с фруктозой образует сахарозу – основную транспортную форму углеводов в растении. В клубни сахароза доставляется по флоэме (апопластный путь), где в межклеточном пространстве расщепляется до глюкозы и фруктозы, которые затем проникают в клетки паренхимы. Глюкоза служит в дальнейшем субстратом для синтеза крахмала в амилопластах. Однако при воздействии пониженных температур крахмал в клубнях картофеля разрушается до редуцирующих сахаров. Параллельно этому процессу идет ресинтез сахарозы до глюкозы и фруктозы за счет фермента кислой вакуолярной инвертазы (β-фруктофуранозидазы), кодируемой геном Pain-1. В совокупности эти процессы приводят к избыточному накоплению моносахаров в клубнях картофеля, так называемому холодовому осахариванию (cold-induced sweetening). При этом создаются условия для интенсивного образования меланоидинов, вызывающих потемнение мякоти картофеля, что значительно ухудшает товарное качество продукта. Таким образом, изучение гена Pain-1, кодирующего вакуолярную инвертазу, а именно, его идентификация и анализ структуры – важная задача, необходимая для поиска доноров, устойчивых к холодовому осахариванию. Первоочередная задача для этого – разработка и тестирование праймерных комбинаций, позволяющих амплифицировать полноразмерный ген у диких видов картофеля, а также сортов и линий культивируемого картофеля (S. tuberosum). В данной работе приведены результаты разработки и тестирования универсальных праймеров, с помощью которых можно амплифицировать как полноразмерные гены-ортологи, так и фрагменты гена Pain-1, а также подобраны оптимальные условия для проведения ПЦР реакции. Было разработано 6 праймерных комбинаций (PainF – PainR, PainF – Pain1exR, Pain1exF – Pain3exR, Pain2inF – Pain2inR, Pain3exF – Pain5exR, Pain5exF – PainR), среди которых комбинация PainF – PainR позволяла амплифицировать полноразмерный ген, остальные – внутренние и будут использованы в дальнейшем при секвенировании фрагментов исследуемого гена. Эти праймеры были успешно протестированы на 15 образцах, включающих представителей пяти дикорастущих видов картофеля (S. gourlay, S. chacoense, S. pinnatissectum, S. stoloniferum, S. vernei) и десяти сортов российской и зарубежной селекции (Гала, Ласунок, Ред Скарлетт, Рассет Бербанк, Мирас, Башкирский, Жуковский ранний, Матушка, Елизавета, Сударыня).The purpose of research is design and testing of universal primers for PCR amplification of full-length-fructofuranozidase orthologs genes (acid vacuolar invertase) in wild species and potato (Solanum tuberosum) varieties. Starch is the main source of energy and a reserve carbohydrate, that accumulates in tubers amyloplasts. Glucose molecule, produced by photosynthesis, reacts with fructose and forms sucrose, which is the main transport type of carbohydrates in the plant. In the tuber, sucrose is delivered via phloem (apoplast), where it splits into glucose and fructose, which then go to the parenchyma cells. Glucose is a further substrate for the starch synthesis in amyloplasts. However, low temperatures influence on potato tubers leads to starch break down to reducing sugars. In parallel to this process there is happens resynthesis of sucrose to glucose and fructose by acid vacuolar invertase enzyme (β-fructofuranosidase) encoded by Pain-1 gene. Together, these processes lead to an excessive accumulation of monosaccharides in potato tubers. This process also called as cold-induced sweetening. It creates conditions for the intensive formation of melanoidins, which cause a potato tubers darkening, which considerably impairs the commercial quality of the product. Thus, the study Pain-1 gene that encodes the vacuolar invertase (its identification and structure analysis) is an important task required for the search of donors resistant to cold-induced sweetening. The primary task for this is the design and testing of primer combinations that allow to amplify the full-length gene in wild potato species, varieties and lines of cultivated potato. In this work, we develop and test universal primers, that can amplify both full-length orthologs and fragments of the Pain-1 gene, and also select the optimal conditions for carrying out the PCR reaction. Summary. The purpose of research is design and testing of universal primers for PCR amplification of full-length-fructofuranozidase orthologs genes (acid vacuolar invertase) in wild species and potato (Solanum tuberosum) varieties. Starch is the main source of energy and a reserve carbohydrate, that accumulates in tubers amyloplasts. Glucose molecule, produced by photosynthesis, reacts with fructose and forms sucrose, which is the main transport type of carbohydrates in the plant. In the tuber, sucrose is delivered via phloem (apoplast), where it splits into glucose and fructose, which then go to the parenchyma cells. Glucose is a further substrate for the starch synthesis in amyloplasts. However, low temperatures influence on potato tubers leads to starch break down to reducing sugars. In parallel to this process there is happens resynthesis of sucrose to glucose and fructose by acid vacuolar invertase enzyme (β-fructofuranosidase) encoded by Pain-1 gene. Together, these processes lead to an excessive accumulation of monosaccharides in potato tubers. This process also called as cold-induced sweetening. It creates conditions for the intensive formation of melanoidins, which cause a potato tubers darkening, which considerably impairs the commercial quality of the product. Thus, the study Pain-1 gene that encodes the vacuolar invertase (its identification and structure analysis) is an important task required for the search of donors resistant to cold-induced sweetening. The primary task for this is the design and testing of primer combinations that allow to amplify the full-length gene in wild potato species, varieties and lines of cultivated potato. In this work, we develop and test universal primers, that can amplify both full-length orthologs and fragments of the Pain-1 gene, and also select the optimal conditions for carrying out the PCR reaction. In total 6 primer combinations were designed (PainF - PainR, PainF - Pain1exR, Pain1exF - Pain3exR, Pain2inF - Pain2inR, Pain3exF - Pain5exR, Pain5exF - PainR), where PainF - PainR primer combination allowed to amplify a full-sized gene, the rest are internal and will be used in the further fragments sequencing of the β-fructofuranosidase gene. These primers were successfully tested on 15 samples, including five wild species of potato (S. gourlay, S. chacoense, S. pinnatissectum, S. stoloniferum, S. vernei) and ten varieties of Russian and foreign breeding (Gala, Lasunok, Red Scarlet , Rasset Burbank, Miras, Bashkirsky, Zhukovsky ranniy, Matushka, Elizaveta, Sudaryna).

An investigation of resistance to the white potato cyst-nematode

1982 ◽  
Vol 99 (2) ◽  
pp. 325-328 ◽  
Author(s):  
M. F. B. Dale ◽  
M. S. Phillips
Keyword(s):  
Solanum Tuberosum ◽  
Major Gene ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Screening Methods ◽  
Inheritance Of Resistance ◽  
White Potato

SUMMARYThe inheritance of resistance to Globodera pallida was studied in seedling progenies derived from Solanum tuberosum ssp. andigena CPC 2802 (H3) and S. vernei and compared with resistance to G. rostochiensis derived from S. tuberosum ssp. andigena CPC 1673(H1). The resistance of CPC 2802 was originally thought to be due to a major gene (H3), but results presented here demonstrate that it and that derived from S. vernei are inherited in a similar manner quite distinct from the major gene inheritance from CPC 1673 (HI). It is concluded that the resistances derived from CPC 2802 and S. vernei are both polygenic in nature. These findings are discussed in relation to breeding policy and screening methods.

Sign in / Sign up

Export Citation Format

Share Document