scholarly journals Tight Physical Linkage of the Nematode Resistance Gene Gpa2 and the Virus Resistance Gene Rx on a Single Segment Introgressed from the Wild Species Solanum tuberosum subsp. andigena CPC 1673 into Cultivated Potato

1999 ◽  
Vol 12 (3) ◽  
pp. 197-206 ◽  
Author(s):  
Jeroen Rouppe van der Voort ◽  
Konstantin Kanyuka ◽  
Edwin van der Vossen ◽  
Abdelhafid Bendahmane ◽  
Paul Mooijman ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Resistance Gene ◽  
Potato Virus ◽  
Wild Species ◽  
Potato Virus X ◽  
Globodera Pallida ◽  
Cyst Nematodes ◽  
Total Size ◽  
Bac Clones ◽  
Mapping Populations

Comparative mapping studies within several plant genera have shown that genes for resistance to taxonomically diverse pathogens are often co-localized on the maps of these genotypes. Here we describe the genetic analysis of such a gene cluster on chromosome 12 of potato. This cluster harbors the resistance loci Gpa2 and Rx, which confer resistance to the potato cyst nematode Globodera pallida and potato virus X (PVX), respectively. By screening two mapping populations with 10 CAPS (cleaved amplified polymorphic sequence) markers derived from AFLP (amplified fragment length polymorphism) markers and the ends of overlapping bacterial artificial chromosome (BAC) clones, it is demonstrated that Gpa2 and Rx are tightly linked. From a recombination fraction of 1/3386, found after screening two different mapping populations, a genetic distance of 0.02 cM between Gpa2 and Rx was calculated. The physical length of this genetic interval does not exceed 300 kb of DNA as determined by the total size of overlapping BAC clones in the Rx/Gpa2 contig. Evidence is provided that both genes have been introgressed from a single wild species clone, Solanum tuberosum subsp. andigena CPC 1673. This suggests that these genes underlying the disease resistance specificities to potato virus X and potato cyst nematodes have a common evolutionary origin.

scholarly journals Salicylic Acid Is Involved in the Nb-Mediated Defense Responses to Potato virus X in Solanum tuberosum

2010 ◽  
Vol 23 (4) ◽  
pp. 394-405 ◽  
Author(s):  
Gerardo Sánchez ◽  
Nadia Gerhardt ◽  
Florencia Siciliano ◽  
Adrián Vojnov ◽  
Isabelle Malcuit ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus X ◽  
Defense Responses ◽  
Avirulent Strain ◽  
Dependent Manner ◽  
Hypersensitive Resistance ◽  
Potato Plants ◽  
Transgenic Lines

To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species–generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.

scholarly journals SEARCH FOR RESISTANCE SOURCES TO GLOBODERA PALLIDA AND POTATO VIRUS X IN THE COLLECTION OF POTATO VARIETIES USING MOLECULAR MARKERS

2019 ◽  
Vol 2 (1) ◽  
pp. 42-48 ◽  
Author(s):  
N. S Klimenko ◽  
◽  
Т. А. Gavrilenko ◽  
L. I. Kostina ◽  
F. T. Mamadbokirova  ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Potato Virus ◽  
Potato Virus X ◽  
Globodera Pallida ◽  
Potato Varieties ◽  
Resistance Sources

scholarly journals Detección serológica y molecular del Potato virus X (PVX) en tubérculos-semilla de papa (Solanum tuberosum L. y Solanum phureja Juz. Bukasov) en Antioquia, Colombia

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Daniela García Ruíz ◽  
Manuel Andrés Olarte Quintero ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Alejandro Marín Montoya
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Solanum Tuberosum L ◽  
Potato Virus X ◽  
Solanum Phureja ◽  
Das Elisa

<p>El Potato virus (PVX) es uno de los virus más limitantes del cultivo de la papa en el mundo. Es transmitido solamente por contacto y por tubérculo-semilla. Su control se fundamenta en la siembra de tubérculos certificados por su sanidad viral y en la disponibilidad de metodologías de diagnóstico altamente sensibles. En este trabajo se evaluó la prevalencia del PVX en cuatro diferentes tejidos de tubérculos de Solanum tuberosum subsp. andigena var. Diacol-Capiro y S. phureja var. Criolla Colombia utilizando pruebas de DAS-ELISA para 128 submuestras y de RT-qPCR para 32 grupos de submuestras (4 submuestras/grupo). Los resultados de las pruebas serológicas  indicaron la presencia de PVX en el 6,25 y 50% de las submuestras analizadas para la variedad Diacol-Capiro y Criolla Colombia, respectivamente; mientras que los niveles de prevalencia del PVX utilizando la detección por RT-qPCR fueron del 93,75%, independientemente de la variedad de papa y del tejido evaluado. Los valores promedio del ciclo umbral (Ct) en las RT-qPCR fueron de 25,6 (Ct=18,02 a 34,49) y el análisis de las curvas de desnaturalización permitió identificar dos variantes del virus con valores de Tm de 79,5±1°C y 83,7±1°C. La secuenciación de los amplicones obtenidos por RT-qPCR para los controles positivos y para dos de las muestras, confirmó su naturaleza viral. Estos resultados señalan unos muy altos niveles de prevalencia de PVX en el material de siembra de papa en Antioquia y la necesidad de fortalecer los programas de certificación de semilla con pruebas de detección como RT-qPCR.</p>

Molecular screening of potato varieties bred by Falenki Breeding station for resistance to phytopathogens

2021 ◽  
Vol 22 (3) ◽  
pp. 340-350
Author(s):  
A. V. Bakulina ◽  
L. S. Savintseva ◽  
O. N. Bashlakova ◽  
N. F. Sintsova
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Potato Virus ◽  
Resistance Genes ◽  
Dna Marker ◽  
Potato Virus X ◽  
Pcr Analysis ◽  
Gene Marker ◽  
Breeding Station ◽  
Short Period

The genotypes of potatoes bred by Falenki Breeding station were studied for the presence of resistance genes markers to the following pathogens: Globodera rostochiensis, Globodera pallidа, Synchytrium endobioticum, potato virus X (PVХ) and potato virus Y (PVY). The method of multiplex PCR analysis was used. The varieties Shurminsky 2, Alisa, Viza, Chayka, Ognivo, Darik, Gloriya, Golubka, Virazh and a promising variety sample 56-09 were studied. In most (8 out of 10) genotypes, marker linked to the Sen1 gene of resistance to S. endobioticum was identified. DNA marker of the G. rostochiensis resistance gene (H1) and the G. pallida resistance gene marker (Gpa2) were found in six genotypes. The marker of the PVX resistance gene (Rx1) was detected in the varieties Shurminsky 2, Alisa, Chayka, Golubka, and Virazh. It has been established that none of the studied potato genotypes carries markers RYSC3, Ry186, YES3-3A linked to the PVY resistance genes. Although in the field, resistance was detected in the samples Chayka, Darik, Virazh, Alisa. Molecular markers linked to the largest number of resistance genes studied (H1, Gpa2, Sen1, and Rx1) were identified in the varieties Shurminsky 2, Golubka, and Virazh. Among the DNA markers used in the work, the data of potato genotype assessment using markers of virus resistance genes (PVX, RYSC3, Ry186, YES3-3A) were less consistent with field observations. The use of molecular markers makes it possible to determine the presence of resistance genes and assess the prospects of a sample in a short period of time, but, at the same time, requires careful choice of a DNA marker that is highly correlated with the manifestation of the trait.

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