scholarly journals Short communication. Introgression of late blight (Phytophthora infestans L.) resistance from tuber-bearing Solanum wild species into cultivated potato

2011 ◽  
Vol 9 (1) ◽  
pp. 193 ◽  
Author(s):  
J. Gabriel ◽  
J. I. Ruiz de Galarreta ◽  
R. Lopez-Pardo ◽  
L. Barandalla ◽  
C. Alvarado ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Wild Species ◽  
Short Communication ◽  
Cultivated Potato

Foliar and tuber assessment of late blight (Phytophthora infestans (Mont.) de Bary) reaction in cultivated potato (Solanum tuberosum L.)

2002 ◽  
Vol 45 (2-4) ◽  
pp. 215-224 ◽  
Author(s):  
D. S. Douches ◽  
W. W. Kirk ◽  
M. A. Bertram ◽  
J. J. Coombs ◽  
B. A. Niemira
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Cultivated Potato

Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools

2008 ◽  
Vol 88 (4) ◽  
pp. 611-619 ◽  
Author(s):  
Qin Chen ◽  
H. Y. Li ◽  
Y. Z. Shi ◽  
D. Beasley ◽  
B. Bizimungu ◽  
...  
Keyword(s):  
Late Blight ◽  
Protoplast Fusion ◽  
Insect Resistance ◽  
Colorado Potato Beetle ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Fusion System ◽  
Cultivated Potato ◽  
Potato Species ◽  
Potato Beetle

Somatic hybridization through protoplast fusion is an important alternative approach for overcoming sexual incompatibility between diploid Solanum species and cultivated potatoes. However, compared with other potato species, methods for protoplast isolation and plant generation for several Mexican wild diploid potato species are not well established. In this study, a systematic procedure was designed for the isolation of a large number of high-quality protoplasts from various Mexican wild species that carry high levels of disease (late blight) and insect [Colorado potato beetle (CPB)] resistance. Using this procedure, an effective potato protoplast fusion system was developed to produce new somatic hybrids between two Mexican, one Argentina wild species, and cultivated potato clones. Regenerated plants and somatic hybrids were obtained at a high frequency from the protoplasts of the diploid wild species and their fused cells with S. tuberosum. Morphological, cytological and molecular marker analyses demonstrated that somatic hybrids were successfully obtained from the cell fusion of S. tuberosum and the diploid species S. pinnatisectum, S. cardiophyllum, and S. chacoense. Assessment of disease and insect reactions demonstrated that several of the protoplast-derived clones and somatic hybrids showed a higher level of resistance to both late blight and CPB than was found in S. tuberosum, confirming that the protoplast system is a powerful tool in potato breeding programs for the development of disease and insect resistance. This new fusion system provides breeders with opportunities to transfer disease and insect resistance genes from Mexican wild species into cultivated potato. Key words: Somatic hybrid, protoplast, fusion, potato, Solanum, late blight, disease resistance, Colorado potato beetle insect resistance

scholarly journals Late Blight Resistance Evaluation and Genome-Wide Assessment of Genetic Diversity in Wild and Cultivated Potato Species

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanfeng Duan ◽  
Shaoguang Duan ◽  
Jianfei Xu ◽  
Jiayi Zheng ◽  
Jun Hu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Gene Pool ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Challenge Inoculation ◽  
Cultivated Potato ◽  
Resistant Varieties

Late blight, caused by the oomycete Phytophthora infestans, is the most devastating disease in potato-producing regions of the world. Cultivation of resistant varieties is the most effective and environmentally friendly way to control potato late blight disease, and identification of germplasms with late blight resistance and clarification their genetic relationship would promote the development of the resistant varieties. In this study, a diverse population of 189 genotypes with potential late blight resistance, consisting of 20 wild species and cultivated Solanum tuberosum Andigenum group and Chilotanum group, was screened for the presence of late blight resistance by performing challenge inoculation with four Phytophthora infestans isolates including one 13_A2 isolate, CN152. Ten elite resources with broad-spectrum resistance and 127 with isolate-specific resistance against P. infestans were identified. To improve the available gene pool for future potato breeding programs, the population was genotyped using 30 simple sequence repeat (SSR) markers covering the entire potato genome. A total of 173 alleles were detected with an average of 5.77 alleles per locus. Structure analysis discriminated the 189 potato genotypes into five populations based on taxonomic classification and genetic origin with some deviations. There was no obvious clustering by country of origin, ploidy level, EBN (endosperm balance number) value, or nuclear clade. Analysis of molecular variance showed 10.08% genetic variation existed among populations. The genetic differentiation (Fst) ranged from 0.0937 to 0.1764, and the nucleotide diversity (π) was 0.2269 across populations with the range from 0.1942 to 0.2489. Further genotyping of 20K SNP array confirmed the classification of SSRs and could uncover the genetic relationships of Solanum germplasms. Our results indicate that there exits abundant genetic variation in wild and cultivated potato germplasms, while the cultivated S. tuberosum Chilotanum group has lower genetic diversity. The phenotypic and genetic information obtained in this study provide a useful guide for hybrid combination and resistance introgression from wild gene pool into cultivated species for cultivar improvement, as well as for germplasm conservation efforts and resistance gene mining.

Discerning strains of Phytophthora infestans the causative agent of potato late blight by SSCP analysis of virulence genes

2018 ◽  
Vol 34 (6) ◽  
pp. 4-11
Author(s):  
V.K. Chizhik ◽  
◽  
E.A. Sokolova ◽  
V.V. Martynov ◽  
M.A. Kuznetsova ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Causative Agent ◽  
Virulence Genes ◽  
Potato Late Blight ◽  
Sscp Analysis

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays

Plant Disease ◽  
2016 ◽  
Vol 100 (7) ◽  
pp. 1482-1491 ◽  
Author(s):  
Marie-Claude Gagnon ◽  
Lawrence Kawchuk ◽  
D. Mathieu Tremblay ◽  
Odile Carisse ◽  
Giovanna Danies ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Rapid Assessment ◽  
Nucleotide Polymorphisms ◽  
Blind Test ◽  
Phytophthora Spp ◽  
Allele Specific ◽  
Flanking Sequences ◽  
Pcr Assays ◽  
And Control

Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.

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