Regeneration of pigeonpea (Cajanus cajan) from cotyledonary node via multiple shoot formation

1994 ◽  
Vol 13 (11) ◽  
Author(s):  
N. Shiva prakash ◽  
Deepak Pental ◽  
Neera Bhalla-Sarin
Keyword(s):  
Cajanus Cajan ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Multiple Shoot Formation

scholarly journals Multiple Shoot Formation from Somatic Embryo Explants of Eastern Redbud (Cercis canadensis L.)

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 548C-548
Author(s):  
Karsedis Distabanjong ◽  
Robert L. Geneve
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Multiple Shoot Formation ◽  
Cercis Canadensis ◽  
Eastern Redbud ◽  
High Degree ◽  
Number Of Shoots

Somatic embryos from Eastern redbud show a high degree of malformation during development and a low conversion rate to seedlings. This problem is common with somatic embryo systems, especially with legume species. A procedure for multiple shoot formation from somatic embryo explants of Eastern redbud was developed that bypasses the need for germination to recover plantlets. Somatic embryo explants cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) produced more shoots than either treatment alone. The highest number of shoots (3.3 to 3.4 shoots per explant) was obtained from partially desiccated and wounded explants treated with a combination of 5 or 10 M BA and 0.5 or 1.0 M TDZ for 20 days before being transferred to the same medium without TDZ. The number of shoots formed was increased from 1.5 to 3.2 shoots per explant by cutting through the cotyledonary node prior to culture. In addition, the frequency of explants forming shoots was increased by desiccation of somatic embryo explants to ≈50% moisture and by using somatic embryos with two well-formed cotyledons as explants.

scholarly journals Regeneration and In Vitro Flowering in Brassica Campestris (L.) Var. Bhavani

Our Nature ◽  
1970 ◽  
Vol 5 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Rinki Verma ◽  
RR Singh
Keyword(s):  
Shoot Apex ◽  
Brassica Campestris ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
In Vitro Flowering ◽  
Cotyledonary Nodes ◽  
Improvement Programs ◽  
Shoot Apex Explants

Multiple shoot formation and in vitro flowering was found in Brassica campestris (L.) var. Bhavani. Maximum numbers of shoots were produced in both cotyledonary node and shoot apex explants on MS-media supplemented with BA (2.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l). Maximum flowering (50%) was noted at IBA (1.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l) in the shoots from cotyledonary nodes. In vitro flowering may contribute in many ways to Brassica Improvement Programs. The shoots rooted well in the half and full strength media each with IBA (1.0 mg/l) and NAA (1.0 mg/l) and the plantlets have been maintained. Keywords: Brassica campestris, In vitro flowering, Regeneration.doi:10.3126/on.v5i1.793Our Nature (2007)5:21-24

scholarly journals Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

2013 ◽  
Vol 1 (1-2) ◽  
pp. 69-72 ◽  
Author(s):  
Nayem Zobayer ◽  
Shamsul H Prodhan ◽  
Saif U Sikdar ◽  
Fazle Azim ◽  
M Ashrafuzzaman
Keyword(s):  
Promising Result ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Fragaria Ananassa ◽  
Benzyl Adenine ◽  
Multiple Shoot Formation ◽  
Basal Media ◽  
Hormonal Concentration

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 2011

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