scholarly journals Regeneration and In Vitro Flowering in Brassica Campestris (L.) Var. Bhavani

Our Nature ◽  
1970 ◽  
Vol 5 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Rinki Verma ◽  
RR Singh
Keyword(s):  
Shoot Apex ◽  
Brassica Campestris ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
In Vitro Flowering ◽  
Cotyledonary Nodes ◽  
Improvement Programs ◽  
Shoot Apex Explants

Multiple shoot formation and in vitro flowering was found in Brassica campestris (L.) var. Bhavani. Maximum numbers of shoots were produced in both cotyledonary node and shoot apex explants on MS-media supplemented with BA (2.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l). Maximum flowering (50%) was noted at IBA (1.5 mg/l) + IAA (1.0 mg/l) + Kn (0.5 mg/l) in the shoots from cotyledonary nodes. In vitro flowering may contribute in many ways to Brassica Improvement Programs. The shoots rooted well in the half and full strength media each with IBA (1.0 mg/l) and NAA (1.0 mg/l) and the plantlets have been maintained. Keywords: Brassica campestris, In vitro flowering, Regeneration.doi:10.3126/on.v5i1.793Our Nature (2007)5:21-24

Electron Microscope studies (TEM and SEM) of in vitro multiplication of Lavandula stoechas L.: I. regeneration from seedling shoot apex explants

1990 ◽  
Vol 48 (3) ◽  
pp. 684-685
Author(s):  
J. F. Mesquita ◽  
M. L. Guimarães ◽  
J.D. Santos Dias
Keyword(s):  
Shoot Apex ◽  
Basal Medium ◽  
Shoot Formation ◽  
Distal Segment ◽  
Multiple Shoot ◽  
Fluorescent Light ◽  
Lavandula Stoechas ◽  
Shoot Apex Explants ◽  
Electron Microscopes

Available data concerning “in vitro” multiplication of Lavandula sps. are scarce and do not involve cytological studies. In this work, shoot initiation from shoot apex explants of Lavandula stoechas is analysed, for the first time, in light and electron microscopes (TEM, SEM). Following habitual sterilization, seeds of Lavandula stoechas were germinated on MS basal medium with 2% sucrose (W/v). From 9-days old seedlings, explants (shoot tips including a small distal segment of hypocotyl - fig. 1) were aseptically excised and cultivated in that basal medium added with organic components as in B5 medium of Gamborg et al., 10% (V/v) of coconut milk, 0.8% of agar and benzyl-amino-purine (BAP) at different concentrations (0-4 mg/1). The pH was adjusted to 5.6 and cultures were maintained at 25±1°C with fluorescent light (2000 lux) for 16 h/day.High frequency of multiple shoot formation was achieved on media supplemented with BAP, the concentration of 1mg/l showing to be the optimal one (Table 1).

scholarly journals In vitro Regeneration and Flower Induction on Solanum nigrum L. from Pachamalai hills of Eastern Ghats

1970 ◽  
Vol 18 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Amzad Basha Kolar ◽  
L . Vivekanandan ◽  
Ghouse Basha M
Keyword(s):  
In Vitro Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Solanum Nigrum ◽  
In Vitro Flowering ◽  
Eastern Ghats ◽  
Solanum Nigrum L

 Explants of Solanum nigrum L., collected from Pachamalai hills callused successfully on MS basal medium supplemented with IAA and BAP. The highest frequency of green compact callus and multiple shoots were obtained on MS containing 2.0 mg/l IAA and 0.5 mg/l BAP. The callus when cultured on MS basal medium fortified with different concentrations of BAP (3.0 - 8.0 mg/l) and IAA (0.5 mg/l) showed multiple shoot formation. The highest frequency of multiple shoots was obtained on MS containing 6.0 mg/l BAP and 0.5 mg/l IAA. For in vitro flowering, the node explants were cultured on MS fortified with different concentrations of BAP (2.0 - 7.0 mg/l) and NAA (0.5 mg/l). The highest number of multiple shoots were obtained in MS supplemented with 6.0 mg/l BAP and 0.5 mg/l NAA. The in vitro flowering was observed on MS containing 2,4-D and BAP 1.5 mg/l, respectively. The best rooting was obtained on MS containing 0.5 mg/l IBA. The well-rooted plants were hardened and finally planted in the garden.  Key words: In vitro studies, Medicinal plant, Solanum nigrum, Node, Callus D.O.I. 10.3329/ptcb.v18i1.3264 Plant Tissue Cult. & Biotech. 18(1): 43-48, 2008 (June)

scholarly journals In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants

2014 ◽  
Vol 6 (1) ◽  
pp. 159-163 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Apex ◽  
Indole Acetic Acid ◽  
Withania Somnifera ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Hardening Process ◽  
Shoot Apex Explants

In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.

scholarly journals Developing a rapid and highly efficient cowpea regeneration and transformation system using embryonic axis explants

2019 ◽  
Author(s):  
Ping Che ◽  
Shujun Chang ◽  
Marissa K. Simon ◽  
Zhifen Zhang ◽  
Ahmed Shaharyar ◽  
...  
Keyword(s):  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Embryonic Axis ◽  
Sub Saharan Africa ◽  
Vector System ◽  
Transformation System ◽  
Highly Efficient ◽  
Cotyledonary Nodes ◽  
Wide Range

SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saharan Africa and Asia. Despite decades of effort, genetic engineering of cowpea is still challenging due to inefficient in vitro shoot regeneration, Agrobacterium-mediated T-DNA delivery and transgenic selection. Here, we report a rapid and highly efficient cowpea transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem by cutting through the middle of the epicotyl stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. Furthermore, the application of a ternary transformation vector system using an optimized pVIR accessory plasmid provided high levels of Agrobacterium-mediated gene delivery. The utilization of spectinomycin as the selection agent enabled more efficient transgenic selection and plant recovery. Transgenic cowpea shoots developed exclusively from the cotyledonary nodes at high frequencies of 4.5 to 37% across a wide range of cowpea genotypes. We believe that the transformation principles established in this study could also be applied to other legumes to increase transformation efficiencies.

scholarly journals Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection

2018 ◽  
Author(s):  
Soledad Mora Vásquez ◽  
Silverio García-Lara ◽  
Guy A. Cardineau
Keyword(s):  
Shoot Organogenesis ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ash Content ◽  
Phenotypic Traits ◽  
Transient Transformation ◽  
Seed Traits ◽  
Best Response

Background and Aims: Soybean regeneration and transformation are considered highly genotype-specific; however, little is known about desirable seed traits that could be useful indicators of their regeneration and transformation capacities. In the present study, eight commercially available Mexican soybean varieties, as well as the Jack genotype as a control, were phenotypically characterized to determine the seed traits that are important factors for their in vitro performance and susceptibility to Agrobacterium tumefaciens infection.Methods: Grain dimensions, seed weight, moisture, oil, protein, ash, carbohydrate content and macro and micro elements (Mg, K, Ca, P, Na, Mn, Fe, Cu and Zn) were measured and correlated with the corresponding direct shoot organogenesis capability and Agrobacterium infection susceptibility using the cotyledonary node as explant.Key results: Notably, the ash content was the only important factor that inversely correlated with the capacity for shoot organogenesis, whereas carbohydrate and phosphorus content were positively correlated with susceptibility to Agrobacterium infection. The best response in terms of multiple shoot formation and Agrobacterium transient transformation was observed with the soybean varieties Huasteca-100, Nainari and Suaqui-86, which have lower ash content and a higher carbohydrate and phosphorus content.Conclusions: In most reported studies, seed phenotypic traits have been overlooked as factors that influence their regeneration and transformation potential. In the present study, we present evidence of associations between some specific seed traits and regeneration and transient transformation of soybean.

scholarly journals In vitro regeneration of three varieties of Brassica campestris L. grown in Bangladesh

2020 ◽  
Vol 55 (3) ◽  
pp. 181-188
Author(s):  
B Goswami ◽  
MI Hoque ◽  
S Khan ◽  
RH Sarker
Keyword(s):  
Shoot Regeneration ◽  
Brassica Campestris ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Root Induction ◽  
Regeneration System ◽  
Cotyledonary Leaf ◽  
Best Response

A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

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