Anaerobic fermentation of fructose in a mixed culture of fermentative and methanogenic bacteria

1989 ◽  
Vol 30 (2) ◽  
Author(s):  
A.Ch. Dubach ◽  
K. Schneider ◽  
R. Bachofen
Author(s):  
Abdollah Hajizadeh ◽  
Noori M. Cata Saady ◽  
Sohrab Zendehboudi ◽  
Rajinikanth Rajagopal ◽  
Yung-Tse Hung

2020 ◽  
Vol 300 ◽  
pp. 122702 ◽  
Author(s):  
Doan Thanh Ta ◽  
Chiu-Yue Lin ◽  
Thi Minh Ngoc Ta ◽  
Chen-Yeon Chu

Author(s):  
V. Kofman

В КНР ведутся активные исследования по разработке технологии ферментации избыточного активного ила с получением водорода. Процесс анаэробной ферментации состоит из трех основных стадий: гидролиз, образование водорода и кислот, образование метана. На стадии гидролиза происходит образование низкомолекулярных веществ из высокомолекулярного крахмала, волокон и белков. На стадии образования водорода и кислот гидрогеногенные и ацидогенные бактерии ферментируют низкомолекулярные вещества с образованием ряда органических кислот, водорода и диоксида углерода. На стадии образования метана метаногенные бактерии метаболизируют продукты, образовавшиеся на предыдущих стадиях с выделением метана и диоксида углерода. В результате получить водород можно только путем ингибирования активности метаногенных бактерий, не оказывая при этом воздействия на активность гидрогеногенных бактерий. С учетом этих обстоятельств разрабатывают способы интенсификации производства биоводорода. Основные усилия в данной области направлены на поиск штаммов с высокой эффективностью анаэробной ферментации. Другим направлением является выбор способа предварительной обработки активного ила из числа тепловой, кислотной, щелочной, СВЧ-обработки, стерилизации и ультразвуковой обработки. Значительные перспективы связывают с использованием консорциума микроорганизмов и смешанного субстрата, содержащего наряду с осадками сточных вод пищевые отходы, солому или навоз. В Индии получило развитие направление обработки сточных вод различных промышленных производств с получением обогащенной липидами биомассы для последующего производства биодизельного топлива. Исследования проведены с использованием бактерий Rhodococcus opacus, дрожжей Rhodosporidium kratochvilovae и микроводорослей Desmodesmus sp.In China, active research is underway for developing a technology for excess activated sludge fermentation to obtain hydrogen. The process of anaerobic fermentation includes three main stages: hydrolysis, formation of hydrogen and acids, and methane generation. At the hydrolysis stage, the formation of low-molecular substances from high-molecular starch, fibers and proteins. At the stage of the hydrogen and acids formation hydrogenogenic and acetogenic bacteria ensure the fermentation of low-molecular substances with the formation of a number of organic acids, hydrogen and carbon dioxide. At the stage of methane generation, methanogenic bacteria metabolize the products formed in the previous stages with the release of methane and carbon dioxide. As a result, hydrogen can be obtained only by inhibiting the activity of methanogenic bacteria eliminating the impact on the activity of hydrogenogenic bacteria. Considering these circumstances methods are being developed to enhance the production of biohydrogen. The main efforts in this area aim at finding strains with high efficiency of anaerobic fermentation. Another direction is choosing a method of activated sludge pre-treatment from among thermal, acid, alkaline, microwave treatment, sterilization and ultrasonic treatment. Significant prospects are associated with the use of a consortium of microorganisms and mixed substrate containing, along with wastewater sludge, food waste, straw or manure. In India, the technologies of processing various types of industrial wastewater with the production of biomass enriched with lipids for the subsequent production of biodiesel have been on the march. The studies have been performed using Rhodococcus opacus bacteria, Rhodosporidium kratochvilovae yeast and Desmodesmus sp microalgae.


Author(s):  
Tomáš Vítěz ◽  
M. Haitl ◽  
Z. Karafiát ◽  
P. Mach ◽  
J. Fryč ◽  
...  

Biogas is a renewable energy resource with high increasing developed in last few decades. It’s big opportunity for stabilization rural areas, concretely agriculture sector. This technology can decentralize supply of energy. The number of operated biogas plants is rapidly increasing. Biogas plants require a high level of intensity and stableness of the process of anaerobic fermentation with biogas production for efficiency treatment, also for good quality of development biogas and fertilization effect of the rest of fermentation. If this is not completed the operator has problem to keep the process in optimal condition for anaerobic fermentation. Researchers have tried different techniques to enhance biogas production. In order to achieve the aforementioned state, it is essential to ensure increased activity of microorganisms that contribute to the anaerobic fermentation. The metabolic activity of microorganisms is preconditioned by availability of easily decomposable solids. Adding of bacterial and enzymatic cultures into a fermented substrate represents one of the possibilities. The enzymes contained in this preparation are responsible for better exposing methanogenic bacteria to the material. The tested bio-enzymatic preparation, APD BIO GAS, is a mixture that contains bacteria and enzymes which are essential for the efficient progress of anaerobic fermentation. The reference biogas laboratory of the Mendel University in Brno was used for the purpose of testing of APD BIOGAS in mesophilic conditions of anaerobic fermentation on a substrate consisting of a mixture of maize silage and liquid manure. The producer of this preparation declare enhancement of quality and quantity of developed biogas, elimination of smell level of the rest of fermentation its higher homogenity. For the test were used lab scale fermenters of batch type with work volume 0.12 m3. An increase of biogas production by 15% was determined in connection with addition of the preparation, also with higher decrease of total solids and decrease of organic substance in total solids in the fermenter where were used this preparation.


2010 ◽  
Vol 18 (3) ◽  
pp. 632-636
Author(s):  
Jin-Cai SHI ◽  
Xin-Di LIAO ◽  
Yin-Bao WU

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