Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro

1984 ◽  
Vol 3 (6) ◽  
pp. 222-225 ◽  
Author(s):  
P. K. Gupta ◽  
S. V. Kendurkar ◽  
V. M. Kulkarni ◽  
M. V. Shirgurkar ◽  
A. F. Mascarenhas
Keyword(s):  
Somatic Embryogenesis ◽  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Cocos Nucifera L

Morphological and agronomical characteristics of coconut (Cocos nucifera L.) palms produced from in vitro cultured zygotic embryos

2013 ◽  
Vol 49 (5) ◽  
pp. 599-604 ◽  
Author(s):  
Yoboué Koffi ◽  
Oulo N’Nan-Alla ◽  
Jean-Louis Konan Konan ◽  
Bernard Malaurie ◽  
Florent Engelmann
Keyword(s):  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Cocos Nucifera L

scholarly journals EFFECT OF DIFFERENT BRANDS OF ACTIVATED CARBON ON GROWTH AND DEVELOPMENT OF COCONUT (Cocos Nucifera L) EMBRYOS IN VITRO

CORD ◽  
1998 ◽  
Vol 14 (02) ◽  
pp. 34
Author(s):  
Zosimo S. Bonaobra III ◽  
Erlinda P. Rillo ◽  
Osmundo D. Orense
Keyword(s):  
Growth And Development ◽  
Cocos Nucifera ◽  
Primary Root ◽  
Shoot Length ◽  
Zygotic Embryos ◽  
Percentage Germination ◽  
Significant Difference ◽  
Initial Culture ◽  
Cocos Nucifera L

The effect of different brands of activated charcoal [Merck GR (Art. 2186), Sigma acid washed (C‑4386), Sigma neutralized (C‑3790), and Duchefa neutralized (C‑1302)] on growth and development ofcoconut zygotic embryos in vitro was evaluated. Analysis of data noted after one month revealed that there was no significant difference on percentage germination, shoot length, and number ofprimary root of cv. Laguna Tall embryos cultured in Y3 liquid medium supplemented with 2.5gll AC of different brands. Length of primary root of embryos cultured in Duchefa neutralized AC was significantly different from those in Sigma neutralized AC only after one month from initial culture. For the succeeding periods (2‑4 months), no significant difiference was observed among the treatments in terms of increment in plant height, number of scale and true leaves and length ofprimary root. Statistical analysis revealed thatpercentage ofseedlings with primary, secondary and tertiary roots did not differ significantly among the treatments 1‑4 months from initial culture. Results suggest that any brand (even alternately) can be used satisfactorily in the in vitro culture of coconut embryos.

Photosynthetic ability of in vitro grown coconut (Cocos nucifera L.) plantlets derived from zygotic embryos

Plant Science ◽  
1997 ◽  
Vol 127 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Karine Triques ◽  
Alain Rival ◽  
Thierry Beulé ◽  
Michel Puard ◽  
Jacques Roy ◽  
...  
Keyword(s):  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Cocos Nucifera L ◽  
Photosynthetic Ability

scholarly journals Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

2020 ◽  
pp. 57-64
Author(s):  
Arnaud Agbidinoukoun ◽  
Euloge Rimson Somakpe ◽  
Serge Sètondji Houedjissin ◽  
Florent Engelmann ◽  
Corneille Ahanhanzo
Keyword(s):  
Survival Rate ◽  
Cocos Nucifera ◽  
Coconut Palm ◽  
Zygotic Embryos ◽  
High Survival ◽  
Regeneration Rate ◽  
Surface Sterilization ◽  
Number Of Leaves ◽  
Cocos Nucifera L

Aims: This study aims to identify the best surface sterilization and evaluate the effect of haustorium suppression on in vitro germination of coconut palm (Cocos nucifera L.) zygotic embryos. Study Design: Survival rate and contamination rate of zygotic embryos after different surface sterilization treatments, regeneration rate and organogenesis through the number of leaves and the length of shoots after haustorium suppression were determined. For data processing, the Analysis of Variance was used to compare the means which were separated according to Tukey test            (P = 0.05). Place and Duration of Study: Coconut fruits (hybrid PB121) were collected 12 to 14 months after controlled pollination from CRAPP (Centre de Recherches Agricoles Plantes Pérennes), station of Sèmè-kpodji in Benin. Experiments were done in Central Laboratory of Plant Biotechnology and Plant Improvement, University of Abomey-Calavi and conducted from june to december in 2019. Methodology: For the zygotic embryos surface sterilization, four treatments combining three concentrations (3%, 6% and 15%) of commercial bleach (Javel la Croix© containing 12° active chlorine) and immersion durations (5 min, 10 min and 20 min) were tested and the survival rate were determined for each treatment after two months culture. The zygotic embryos were then divided in two sets (haustorium excised embryos set and the whole embryos set) and cultured in modified Y3 medium supplemented with 7 g L-1 agar, 2.5 g L-1 activated charcoal, 5% sucrose,  6.10-3 mM 2.4 D (2.4-dichlorophonoxyacetic acid), gibberellic acid and 0.3 mM BAP(6-benzylaminopurine). After five months culture, the regeneration rate, the number of leaves and the length of shouts were recorded. Results: The high survival rate (80%) was obtained with 6% of bleach and 20 min for the immersion duration without pre-disinfection. The suppression of haustorium have significantly increased the number of leaves (4.3 ± 0.02) and the length of shoots (16.2 ±0.7cm) compared to the whole zygotic embryos. Conclusion: This protocol can help to ensure better surface sterilization of zygotic embryos before their in vitro culture and the development of vigorous plantlets in order to improve the slow growth of plantlets, when transferred to the greenhouse or field. 

scholarly journals Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)

2018 ◽  
Vol 17 (27) ◽  
pp. 862-869 ◽  
Author(s):  
Wentoin Alimata Marie Pierre DARAMCOUM ◽  
Konan Jean-Louis KONAN ◽  
Saraka Didier Martial YAO ◽  
Arocha Rosete YAIMA ◽  
Eric-Blanchard Zadjéhi KOFFI ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Cocos Nucifera ◽  
Coconut Palm ◽  
Zygotic Embryos ◽  
Regenerated Plants ◽  
Cocos Nucifera L

GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)

2011 ◽  
Vol 107 (3) ◽  
pp. 461-470 ◽  
Author(s):  
Mayra Montero-Cortés ◽  
Iván Cordova ◽  
Jean-Luc Verdeil ◽  
Valerie Hocher ◽  
América Pech y Ake ◽  
...  
Keyword(s):  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
In Vitro Germination ◽  
Cocos Nucifera L

scholarly journals Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2050
Author(s):  
Sundaravelpandian Kalaipandian ◽  
Zhihua Mu ◽  
Eveline Yee Yan Kong ◽  
Julianne Biddle ◽  
Robyn Cave ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Large Scale ◽  
Cocos Nucifera ◽  
Current Status ◽  
Scale Production ◽  
Promising Alternative ◽  
The Status ◽  
Propagation Methods ◽  
Cocos Nucifera L

Coconut [Cocos nucifera L.] is often called “the tree of life” because of its many uses in the food, beverage, medicinal, and cosmetic industries. Currently, more than 50% of the palms grown throughout the world are senile and need to be replanted immediately to ensure production levels meet the present and increasing demand for coconut products. Mass replanting will not be possible using traditional propagation methods from seed. Recent studies have indicated that in vitro cloning via somatic embryogenesis is the most promising alternative for the large-scale production of new coconut palms. This paper provides a review on the status and prospects for the application of somatic embryogenesis to mass clonal propagation of coconut.

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