Control of Co1E2 plasmid replication: regulation of rep expression by a plasmid-coded antisense RNA

1994 ◽  
Vol 244 (1) ◽  
pp. 49-56 ◽  
Author(s):  
S. Takechi ◽  
H. Yasueda ◽  
T. Itoh
Keyword(s):  
Antisense Rna ◽  
Plasmid Replication ◽  
Replication Regulation

Four-way Junctions in Antisense RNA-mRNA Complexes Involved in Plasmid Replication Control: A Common Theme?

2001 ◽  
Vol 309 (3) ◽  
pp. 605-614 ◽  
Author(s):  
Fabrice A. Kolb ◽  
Eric Westhof ◽  
Bernard Ehresmann ◽  
Chantal Ehresmann ◽  
E.Gerhart H. Wagner ◽  
...  
Keyword(s):  
Antisense Rna ◽  
Plasmid Replication ◽  
Common Theme ◽  
Replication Control

scholarly journals Analysis of the Mechanism of Action of the Antisense RNA That Controls the Replication of the repABC Plasmid p42d

2010 ◽  
Vol 192 (13) ◽  
pp. 3268-3278 ◽  
Author(s):  
Ramón Cervantes-Rivera ◽  
Cristina Romero-López ◽  
Alfredo Berzal-Herranz ◽  
Miguel A. Cevallos
Keyword(s):  
Mechanism Of Action ◽  
Antisense Rna ◽  
Effective Interaction ◽  
Plasmid Replication ◽  
Rhizobium Etli ◽  
Antisense Molecule ◽  
Initiator Protein ◽  
Symbiotic Plasmid ◽  
Protein Encoding ◽  
Encoding Genes

ABSTRACT Replication and segregation of the Rhizobium etli symbiotic plasmid (pRetCFN42d) depend on the presence of a repABC operon, which carries all the plasmid-encoded elements required for these functions. All repABC operons share three protein-encoding genes (repA, repB, and repC), an antisense RNA (ctRNA) coding gene, and at least one centromere-like region (parS). The products of repA and repB, in conjunction with the parS region, make up the segregation system, and they negatively regulate operon transcription. The last gene of the operon, repC, encodes the initiator protein. The ctRNA is a negative posttranscriptional regulator of repC. In this work, we analyzed the secondary structures of the ctRNA and its target and mapped the motifs involved in the complex formed between them. Essential residues for the effective interaction localize at the unpaired 5′ end of the antisense molecule and the loop of the target mRNA. In light of our results, we propose a model explaining the mechanism of action of this ctRNA in the regulation of plasmid replication in R. etli.

scholarly journals Replication and Control of Circular Bacterial Plasmids

1998 ◽  
Vol 62 (2) ◽  
pp. 434-464 ◽  
Author(s):  
Gloria del Solar ◽  
Rafael Giraldo ◽  
María Jesús Ruiz-Echevarría ◽  
Manuel Espinosa ◽  
Ramón Díaz-Orejas
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Dna Sequences ◽  
Antisense Rna ◽  
Bacterial Population ◽  
Replication Initiation ◽  
Plasmid Replication ◽  
Genetic Elements ◽  
Bacterial Plasmids

SUMMARY An essential feature of bacterial plasmids is their ability to replicate as autonomous genetic elements in a controlled way within the host. Therefore, they can be used to explore the mechanisms involved in DNA replication and to analyze the different strategies that couple DNA replication to other critical events in the cell cycle. In this review, we focus on replication and its control in circular plasmids. Plasmid replication can be conveniently divided into three stages: initiation, elongation, and termination. The inability of DNA polymerases to initiate de novo replication makes necessary the independent generation of a primer. This is solved, in circular plasmids, by two main strategies: (i) opening of the strands followed by RNA priming (theta and strand displacement replication) or (ii) cleavage of one of the DNA strands to generate a 3′-OH end (rolling-circle replication). Initiation is catalyzed most frequently by one or a few plasmid-encoded initiation proteins that recognize plasmid-specific DNA sequences and determine the point from which replication starts (the origin of replication). In some cases, these proteins also participate directly in the generation of the primer. These initiators can also play the role of pilot proteins that guide the assembly of the host replisome at the plasmid origin. Elongation of plasmid replication is carried out basically by DNA polymerase III holoenzyme (and, in some cases, by DNA polymerase I at an early stage), with the participation of other host proteins that form the replisome. Termination of replication has specific requirements and implications for reinitiation, studies of which have started. The initiation stage plays an additional role: it is the stage at which mechanisms controlling replication operate. The objective of this control is to maintain a fixed concentration of plasmid molecules in a growing bacterial population (duplication of the plasmid pool paced with duplication of the bacterial population). The molecules involved directly in this control can be (i) RNA (antisense RNA), (ii) DNA sequences (iterons), or (iii) antisense RNA and proteins acting in concert. The control elements maintain an average frequency of one plasmid replication per plasmid copy per cell cycle and can “sense” and correct deviations from this average. Most of the current knowledge on plasmid replication and its control is based on the results of analyses performed with pure cultures under steady-state growth conditions. This knowledge sets important parameters needed to understand the maintenance of these genetic elements in mixed populations and under environmental conditions.

Translational control by antisense RNA in control of plasmid replication

Gene ◽  
1988 ◽  
Vol 72 (1-2) ◽  
pp. 237-240 ◽  
Author(s):  
Kurt Nordström ◽  
E.Gerhart H. Wagner ◽  
Christine Persson ◽  
Pontus Blomberg ◽  
Marie Öhman
Keyword(s):  
Translational Control ◽  
Antisense Rna ◽  
Plasmid Replication

scholarly journals Antisense-RNA-mediated plasmid copy number control in pCG1-family plasmids, pCGR2 and pCG1, in Corynebacterium glutamicum

Microbiology ◽  
2010 ◽  
Vol 156 (12) ◽  
pp. 3609-3623 ◽  
Author(s):  
Naoko Okibe ◽  
Nobuaki Suzuki ◽  
Masayuki Inui ◽  
Hideaki Yukawa
Keyword(s):  
Corynebacterium Glutamicum ◽  
Antisense Rna ◽  
Copy Number ◽  
Shuttle Vector ◽  
Fold Increase ◽  
Plasmid Copy Number ◽  
Plasmid Replication ◽  
Plasmid Copy ◽  
Rna Molecules ◽  
Target Mrnas

pCGR2 and pCG1 belong to different subfamilies of the pCG1 family of Corynebacterium glutamicum plasmids. Nonetheless, they harbour homologous putative antisense RNA genes, crrI and cgrI, respectively. The genes in turn share identical positions complementary to the leader region of their respective repA (encoding plasmid replication initiator) genes. Determination of their precise transcriptional start- and end-points revealed the presence of short antisense RNA molecules (72 bp, CrrI; and 73 bp, CgrI). These short RNAs and their target mRNAs were predicted to form highly structured molecules comprising stem–loops with known U-turn motifs. Abolishing synthesis of CrrI and CgrI by promoter mutagenesis resulted in about sevenfold increase in plasmid copy number on top of an 11-fold (CrrI) and 32-fold (CgrI) increase in repA mRNA, suggesting that CrrI and CgrI negatively control plasmid replication. This control is accentuated by parB, a gene that encodes a small centromere-binding plasmid-partitioning protein, and is located upstream of repA. Simultaneous deactivation of CrrI and parB led to a drastic 87-fold increase in copy number of a pCGR2-derived shuttle vector. Moreover, the fact that changes in the structure of the terminal loops of CrrI and CgrI affected plasmid copy number buttressed the important role of the loop structure in formation of the initial interaction complexes between antisense RNAs and their target mRNAs. Similar antisense RNA control systems are likely to exist not only in the two C. glutamicum pCG1 subfamilies but also in related plasmids across Corynebacterium species.

Control of ColE1 plasmid replication by antisense RNA

1991 ◽  
Vol 7 (1) ◽  
pp. 230-235 ◽  
Author(s):  
G Cesareni
Keyword(s):  
Antisense Rna ◽  
Plasmid Replication ◽  
Cole1 Plasmid

Control of ColE1 plasmid replication by antisense RNA

1991 ◽  
Vol 7 (7) ◽  
pp. 230-235 ◽  
Author(s):  
G. Cesareni ◽  
M. Helmer-Citterich ◽  
L. Castagnoli
Keyword(s):  
Antisense Rna ◽  
Plasmid Replication ◽  
Cole1 Plasmid
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