Optimal use of restriction enzymes in the analysis of human DNA polymorphism

1983 ◽  
Vol 63 (2) ◽  
pp. 162-165 ◽  
Author(s):  
F. Basti�-Sigeac ◽  
G. Lucotte
Keyword(s):  
Dna Polymorphism ◽  
Restriction Enzymes ◽  
Human Dna

Mitochondrial DNA polymorphism detected with the restriction enzymes BstNI and BclI in a French Canadian population

1989 ◽  
Vol 53 (4) ◽  
pp. 319-325 ◽  
Author(s):  
Y. GÉLINAS ◽  
L. TURCOTTE ◽  
C. BOUCHANRD ◽  
M-C. THIBAULT ◽  
F. T. DIONNE
Keyword(s):  
Mitochondrial Dna ◽  
Dna Polymorphism ◽  
Restriction Enzymes ◽  
French Canadian ◽  
Mitochondrial Dna Polymorphism ◽  
Canadian Population

scholarly journals Intraspecific mitochondrial DNA polymorphism within the emerging filamentous fungal pathogen Trichoderma longibrachiatum

2006 ◽  
Vol 55 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Zsuzsanna Antal ◽  
János Varga ◽  
László Kredics ◽  
András Szekeres ◽  
Lóránt Hatvani ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Fungal Pathogen ◽  
Internal Transcribed Spacer ◽  
Dna Polymorphism ◽  
Restriction Enzymes ◽  
Clinical Isolates ◽  
Trichoderma Longibrachiatum ◽  
Mitochondrial Dna Polymorphism ◽  
Mitochondrial Dnas ◽  
Dna Profiles

The genetic diversity of the emerging fungal pathogen Trichoderma longibrachiatum was examined at the level of mitochondrial DNA. The 17 investigated strains, comprising nine clinical and eight non-clinical isolates, exhibited seven and ten different mitochondrial DNA profiles by using the restriction enzymes BsuRI and Hin6I, respectively. The sizes of mitochondrial DNAs varied from 34·9 to 39·5 kb. The discriminatory power of the method was higher than that of internal transcribed spacer sequence analysis and therefore should be more suitable for identification and epidemiological investigations. However, clinical and non-clinical isolates did not form separate clusters on the resulting dendrogram and thus there was no indication of a correlation between genetic structure and pathogenicity of the isolates.

scholarly journals A calculation of fragment lengths obtainable from human DNA with 78 restriction enzymes: an aid for cloning and mapping

1986 ◽  
Vol 14 (11) ◽  
pp. 4691-4692 ◽  
Author(s):  
Radoje Drmanac ◽  
Nenad Petrovic ◽  
Vladimir Glisin ◽  
Radomir Crkvenjakov
Keyword(s):  
Restriction Enzymes ◽  
Human Dna

A novel human DNA polymorphism resulting from transfer of DNA from chromosome 6 to chromosome 16

Genomics ◽  
1990 ◽  
Vol 7 (2) ◽  
pp. 222-234 ◽  
Author(s):  
Zilla Wong ◽  
Nicola J. Royle ◽  
Alec J. Jeffreys
Keyword(s):  
Dna Polymorphism ◽  
Chromosome 6 ◽  
Chromosome 16 ◽  
Human Dna

SEARCH FOR AND USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs) IN AND AROUND THE HUMAN FACTOR IX GENE

1987 ◽  
Author(s):  
M B Liddell ◽  
D S Anson ◽  
D P Lillicrap ◽  
I R Peake
Keyword(s):  
Sequence Variation ◽  
Human Factor ◽  
Repetitive Sequences ◽  
Low Frequency ◽  
Restriction Enzymes ◽  
Factor Ix ◽  
Composite Gels ◽  
Human Dna ◽  
Length Polymorphisms ◽  
Human Factor Ix

5 previously described RFLPs within the factor IX gene have been used for family studies (carrier detection) in 10 haemophilia B kindred. In all DNA from 91 individuals, including 25 obligate or possible carriers, was analysed by digestion with TaqI and XmnI and probing with the intragenomic probe VIII (all probes were provided by Professor G. G. Brownlee, Oxford). When noninformative, additional RFLPs (DdeI;probe XIII and MspI;probe II) were used. Of 12 possible carriers, 11 were diagnosed (6 as carriers, 5 normal). Of the confirmed carriers (6 diagnosed, 13 obligate) 15 were informative (heterozygous and phase known), and the overall incidence of heterozygosity was 72%. The recently reported BamHI RFLP was not found to be useful ( <1.0% frequency).Further RFLPs in and flanking the factor IX gene were sought by two procedures. Firstly cosmid pCHIXα, containing a 40kb insert including the 3' end of the factor IX gene and stretching some 35kb 3' to the gene was used as a large probe, with repetitive sequences being blocked by preannealing the probe with an excess of sonicated, denatured human DNA (Litt and White, PNAS 82, 6206). Results with 25 restriction enzymes (covering an estimated 1038 nucleotides) and DNA from 7 unrelated females were obtained, but only one low frequency PvuII RFLP (frequency about 1%) was identified. Similar experiments with further cosmid probes 3' to the gene are underway. The second technique was developed to analyse small DNA fragments (<1.0kb) generated by frequently cutting restriction enzymes. These fragments were separated on 3.5% polyacrylamide/0.5% agarose composite gels and then electroblotted onto hybond-N. Fragments of 150bp were readily visualised by this procedure. 3 frequently cutting enzymes have been used (Hinfl, Rsal and Mbol), and the blots probed with a factor IX c-DNA probe, or a unique sequence subclone of cosmid pCHIXα. To date no RFLPs have been identified. This search for further useful RFLP has illustrated the paucity of detectable sequence variation within this region of the X-chromosome.

scholarly journals DNA Database of the Nicaraguan Population: Allele Frequencies of Importance in Forensic Genetics

Encuentro ◽  
2010 ◽  
pp. 97-107
Author(s):  
Raquel Vargas-Díaz ◽  
Jorge A Huete-Pérez
Keyword(s):  
Dna Polymorphism ◽  
Forensic Genetics ◽  
Scientific Investigation ◽  
Discrete Groups ◽  
Future Prospects ◽  
Criminal Investigations ◽  
Genetic Fingerprint ◽  
Dna Database ◽  
Human Dna ◽  
Scientific Technical

Scientific-technical development in the field of natural science, specifically the discovery of human DNA polymorphism, has allowed us to identify people by their genetic fingerprint, i.e. their DNA, unique to every individual on earth. Its use in criminal investigations and forensic medicine has brought about the creation of DNA databases for discrete groups, populations and entire nations. In Nicaragua, the Molecular Biology Center of the Universidad Centroamericana has been a pioneer in this area of research, providing support for criminal investigations and resolving innumerable cases of paternity disputes. In this report we present the achievements of ten years of research, highlighting the technical aspects and, in particular, the application of the AmpFlSTR Identifiler system, as well as future prospects for scientific investigation in this area.

Human DNA Polymorphism

1995 ◽  
Vol 332 (5) ◽  
pp. 318-320 ◽  
Author(s):  
David Housman
Keyword(s):  
Dna Polymorphism ◽  
Human Dna
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