Conjugal transfer of plasmid and chromosomal markers between strains of Thiobacillus versutus

1995 ◽  
Vol 30 (4) ◽  
pp. 185-191 ◽  
Author(s):  
Mirosława Włodarczyk ◽  
Ewa Piechucka
Keyword(s):  
Conjugal Transfer ◽  
Thiobacillus Versutus ◽  
Chromosomal Markers

Nomenclature Abstract for Thiobacillus versutus Harrison 1983.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Thiobacillus Versutus

Nomenclature Abstract for Thiobacillus rapidicrescens Katayama-Fujimura et al. 1983 pro synon. Thiobacillus versutus Harrison 1983.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity ◽  
Dorothea Taylor
Keyword(s):  
Thiobacillus Versutus

Linkage of chromosomal markers on 4q with a putative gene determining maximal insulin action in Pima Indians

Diabetes ◽  
1993 ◽  
Vol 42 (4) ◽  
pp. 514-519 ◽  
Author(s):  
M. Prochazka ◽  
S. Lillioja ◽  
J. F. Tait ◽  
W. C. Knowler ◽  
D. M. Mott ◽  
...  
Keyword(s):  
Insulin Action ◽  
Pima Indians ◽  
Putative Gene ◽  
Chromosomal Markers

UV Stimulation of Chromosomal Marker Exchange in Sulfolobus acidocaldarius: Implications for DNA Repair, Conjugation and Homologous Recombination at Extremely High Temperatures

Genetics ◽  
1999 ◽  
Vol 152 (4) ◽  
pp. 1407-1415 ◽  
Author(s):  
Katherine J Schmidt ◽  
Kristen E Beck ◽  
Dennis W Grogan
Keyword(s):  
Homologous Recombination ◽  
Incubation Temperature ◽  
Uv Light ◽  
Dna Lesions ◽  
Sulfolobus Acidocaldarius ◽  
Chromosomal Marker ◽  
Marker Exchange ◽  
Chromosomal Markers ◽  
Rate Of Decay ◽  
Uv Photoproducts

Abstract The hyperthermophilic archaeon Sulfolobus acidocaldarius exchanges and recombines chromosomal markers by a conjugational mechanism, and the overall yield of recombinants is greatly increased by previous exposure to UV light. This stimulation was studied in an effort to clarify its mechanism and that of marker exchange itself. A variety of experiments failed to identify a significant effect of UV irradiation on the frequency of cell pairing, indicating that subsequent steps are primarily affected, i.e., transfer of DNA between cells or homologous recombination. The UV-induced stimulation decayed rather quickly in parental cells during preincubation at 75°, and the rate of decay depended on the incubation temperature. Preincubation at 75° decreased the yield of recombinants neither from unirradiated parental cells nor from parental suspensions subsequently irradiated. We interpret these results as evidence that marker exchange is stimulated by recombinogenic DNA lesions formed as intermediates in the process of repairing UV photoproducts in the S. acidocaldarius chromosome.

scholarly journals Use of a Small Palindrome Genetic Marker to Investigate Mechanisms of Double-Strand-Break Repair in Mammalian Cells

Genetics ◽  
2000 ◽  
Vol 154 (3) ◽  
pp. 1281-1289 ◽  
Author(s):  
Julang Li ◽  
Mark D Baker
Keyword(s):  
Mismatch Repair ◽  
Mammalian Cells ◽  
Indirect Evidence ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Chromosomal Markers ◽  
Vector Borne ◽  
Break Repair ◽  
The One

Abstract We examined mechanisms of mammalian homologous recombination using a gene targeting assay in which the vector-borne region of homology to the chromosome bore small palindrome insertions that frequently escape mismatch repair when encompassed within heteroduplex DNA (hDNA). Our assay permitted the product(s) of each independent recombination event to be recovered for molecular analysis. The results revealed the following: (i) vector-borne double-strand break (DSB) processing usually did not yield a large double-strand gap (DSG); (ii) in 43% of the recombinants, the results were consistent with crossover at or near the DSB; and (iii) in the remaining recombinants, hDNA was an intermediate. The sectored (mixed) genotypes observed in 38% of the recombinants provided direct evidence for involvement of hDNA, while indirect evidence was obtained from the patterns of mismatch repair (MMR). Individual hDNA tracts were either long or short and asymmetric or symmetric on the one side of the DSB examined. Clonal analysis of the sectored recombinants revealed how vector-borne and chromosomal markers were linked in each strand of individual hDNA intermediates. As expected, vector-borne and chromosomal markers usually resided on opposite strands. However, in one recombinant, they were linked on the same strand. The results are discussed with particular reference to the double-strand-break repair (DSBR) model of recombination.

Conjugal transfer of catabolic plasmids by damaged bacterial cells: Rescuing genes for pesticide biodegradation

2021 ◽  
Vol 159 ◽  
pp. 105186
Author(s):  
Boris Parra ◽  
Gonzalo Tortella ◽  
Arnaud Dechesne ◽  
Miguel Martínez
Keyword(s):  
Conjugal Transfer ◽  
Bacterial Cells ◽  
Pesticide Biodegradation ◽  
Catabolic Plasmids

Housewife peritonitis: conjugal transfer of a pathogen

1991 ◽  
Vol 17 (1) ◽  
pp. 69-70 ◽  
Author(s):  
M.S. Dryden ◽  
M. McCann ◽  
I. Phillips
Keyword(s):  
Conjugal Transfer

scholarly journals Conjugal transfer of chromosomal DNA inMycobacterium smegmatis

1998 ◽  
Vol 28 (3) ◽  
pp. 571-582 ◽  
Author(s):  
Linda M. Parsons ◽  
Craig S. Jankowski ◽  
Keith M. Derbyshire
Keyword(s):  
Conjugal Transfer ◽  
Chromosomal Dna

Chromosome mobilization of Legionella pneumophila with RK2::Mu and Tn5-Mob

1992 ◽  
Vol 38 (7) ◽  
pp. 664-671 ◽  
Author(s):  
Clifford S. Mintz ◽  
Chang Hua Zou
Keyword(s):  
Genetic Mapping ◽  
Key Words ◽  
Legionella Pneumophila ◽  
Transposon Tn5 ◽  
Helper Plasmid ◽  
Chromosome Transfer ◽  
Chromosomal Markers

RK2::Mu plasmids and transposon Tn5-Mob were used to mobilize the Legionella pneumophila chromosome. Plate matings between L. pneumophila donors that contained RK2::Mu plasmids and auxotrophic recipients yielded recombinants at fequencies ranging from 10−6 to 10−7 per recipient for the markers tested. The presence of a Mu insertion in the chromosome of donors that harbored RK2::Mu plasmids increased the frequency of chromosome transfer of certain selected markers as compared with strains that contained RK2::Mu alone. Cotransfer experiments with Mu-containing donors and a thymidine and tryptophan auxotroph failed to reveal any linkage between the thy and trp loci in L. pneumophila. A strain that contained a chromosomal Tn5-Mob insertion and helper plasmid pRK24.4 transferred chromosomal markers at frequencies of 10−7 per recipient. These findings suggest that RK2::Mu plasmids and Tn5-Mob may be useful for genetic mapping experiments with L. pneumophila. Key words: Legionella pneumophila, chromosome transfer, Tn5-Mob, RK2::Mu.

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