Characterization of a novel Azorhizobium caulinodans ORS571 two-component regulatory system, NtrY/NtrX, involved in nitrogen fixation and metabolism

1991 ◽  
Vol 231 (1) ◽  
pp. 124-138 ◽  
Author(s):  
K. Pawlowski ◽  
U. Klosse ◽  
F. J. de Bruijn
Keyword(s):  
Nitrogen Fixation ◽  
Regulatory System ◽  
Azorhizobium Caulinodans ◽  
Two Component ◽  
Azorhizobium Caulinodans Ors571

Characterization of the fixABC region of Azorhizobium caulinodans ORS571 and identification of a new nitrogen fixation gene

1988 ◽  
Vol 214 (3) ◽  
pp. 496-502 ◽  
Author(s):  
P. Alexandre Kaminski ◽  
Françoise Norel ◽  
Nicole Desnoues ◽  
Anil Kush ◽  
Giovanni Salzano ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Azorhizobium Caulinodans ◽  
Azorhizobium Caulinodans Ors571 ◽  
Nitrogen Fixation Gene

Molecular characterization of lpxACD and pmrA/B two-component regulatory system in the colistin resistance Acinetobacter baumannii clinical isolates

Gene Reports ◽  
2020 ◽  
Vol 21 ◽  
pp. 100952
Author(s):  
Mohammad Reza Kandehkar Ghahraman ◽  
Hossein Hosseini-Nave ◽  
Omid Azizi ◽  
Mohammad Reza Shakibaie ◽  
Hamid Reza Mollaie ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Regulatory System ◽  
Clinical Isolates ◽  
Colistin Resistance ◽  
Two Component

scholarly journals Identification and characterization of a functional nodD gene in Azorhizobium caulinodans ORS571.

1990 ◽  
Vol 172 (5) ◽  
pp. 2658-2666 ◽  
Author(s):  
K Goethals ◽  
G Van den Eeede ◽  
M Van Montagu ◽  
M Holsters
Keyword(s):  
Azorhizobium Caulinodans ◽  
Azorhizobium Caulinodans Ors571 ◽  
Identification And Characterization

T2056 Characterization of a Two-Component Regulatory System in a Type IV Pili of Etec

2009 ◽  
Vol 136 (5) ◽  
pp. A-630
Author(s):  
Bharani Pandrangi ◽  
Oscar Gomez
Keyword(s):  
Regulatory System ◽  
Type Iv Pili ◽  
Type Iv ◽  
Two Component

scholarly journals Phenotypic and Transcriptional Characterization of the Meningococcal PhoPQ System, a Magnesium-Sensing Two-Component Regulatory System That Controls Genes Involved in Remodeling the Meningococcal Cell Surface

2005 ◽  
Vol 187 (14) ◽  
pp. 4967-4975 ◽  
Author(s):  
J. Newcombe ◽  
J. C. Jeynes ◽  
E. Mendoza ◽  
J. Hinds ◽  
G. L. Marsden ◽  
...  
Keyword(s):  
Cell Surface ◽  
Transcriptional Profiling ◽  
Regulatory System ◽  
Host Environment ◽  
Low Magnesium ◽  
System A ◽  
Solid Media ◽  
Two Component ◽  
Pathogenic Process

ABSTRACT We previously identified and characterized a two-component regulatory system in the meningococcus with homology to the phoP-phoQ system in salmonella and showed that allele replacement of the NMB0595 regulator gene led to loss of virulence, sensitivity to antimicrobial peptides, perturbed protein expression, and magnesium-sensitive growth. On the basis of these findings we proposed that the system should be designated the meningococcal PhoPQ system. Here we further characterized the NMB0595 mutant and demonstrated that it had increased membrane permeability and was unable to form colonies on solid media with low magnesium concentrations, features that are consistent with disruption of PhoPQ-mediated modifications to the lipooligosaccharide structure. We examined the transcriptional profiles of wild-type and NMB0595 mutant strains and found that magnesium-regulated changes in gene expression are completely abrogated in the mutant, indicating that, similar to the salmonella PhoPQ system, the meningococcal PhoPQ system is regulated by magnesium. Transcriptional profiling of the mutant indicated that, also similar to the salmonella PhoPQ system, the meningococcal system is involved in control of virulence and remodeling of the bacterial cell surface in response to the host environment. The results are consistent with the hypothesis that the PhoP homologue plays a role in the meningococcus similar to the role played by PhoP in salmonella. Elucidating the role that the PhoPQ system and PhoPQ-regulated genes play in the response of the meningococcus to the host environment may provide new insights into the pathogenic process.

scholarly journals Molecular and functional characterization of the Azorhizobium caulinodans ORS571 hydrogenase gene cluster

2004 ◽  
Vol 237 (2) ◽  
pp. 399-405 ◽  
Author(s):  
Cecilia Baginsky ◽  
Jose-Manuel Palacios ◽  
Juan Imperial ◽  
Tomás Ruiz-Argüeso ◽  
Belén Brito
Keyword(s):  
Gene Cluster ◽  
Functional Characterization ◽  
Azorhizobium Caulinodans ◽  
Hydrogenase Gene ◽  
Azorhizobium Caulinodans Ors571

scholarly journals Rhizobial Factors Required for Stem Nodule Maturation and Maintenance in Sesbania rostrata-Azorhizobium caulinodans ORS571 Symbiosis

2007 ◽  
Vol 73 (20) ◽  
pp. 6650-6659 ◽  
Author(s):  
Shino Suzuki ◽  
Toshihiro Aono ◽  
Kyung-Bum Lee ◽  
Tadahiro Suzuki ◽  
Chi-Te Liu ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Sesbania Rostrata ◽  
Azorhizobium Caulinodans ◽  
Physiological Mechanisms ◽  
Repeated Observations ◽  
Stem Nodule ◽  
Tropical Legume ◽  
Fixation Activity ◽  
Stem Nodulation ◽  
Azorhizobium Caulinodans Ors571

ABSTRACT The molecular and physiological mechanisms behind the maturation and maintenance of N2-fixing nodules during development of symbiosis between rhizobia and legumes still remain unclear, although the early events of symbiosis are relatively well understood. Azorhizobium caulinodans ORS571 is a microsymbiont of the tropical legume Sesbania rostrata, forming N2-fixing nodules not only on the roots but also on the stems. In this study, 10,080 transposon-inserted mutants of A. caulinodans ORS571 were individually inoculated onto the stems of S. rostrata, and those mutants that induced ineffective stem nodules, as displayed by halted development at various stages, were selected. From repeated observations on stem nodulation, 108 Tn5 mutants were selected and categorized into seven nodulation types based on size and N2 fixation activity. Tn5 insertions of some mutants were found in the well-known nodulation, nitrogen fixation, and symbiosis-related genes, such as nod, nif, and fix, respectively, lipopolysaccharide synthesis-related genes, C4 metabolism-related genes, and so on. However, other genes have not been reported to have roles in legume-rhizobium symbiosis. The list of newly identified symbiosis-related genes will present clues to aid in understanding the maturation and maintenance mechanisms of nodules.

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