Incorporation of tritiated thymidine into mitochondrial DNA of the liver and kidney cells of chickens and mice in tissue culture

1967 ◽  
Vol 10 (4) ◽  
pp. 305-308 ◽  
Author(s):  
T. Nagata ◽  
O. Shibata ◽  
T. Nawa
Keyword(s):  
Tissue Culture ◽  
Mitochondrial Dna ◽  
Tritiated Thymidine ◽  
Kidney Cells ◽  
Liver And Kidney

A second rubella vaccine

1971 ◽  
Vol 9 (13) ◽  
pp. 52-52
Keyword(s):  
Tissue Culture ◽  
Pregnant Women ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Animal Cells ◽  
Rubella Vaccine ◽  
Human Diploid Cells ◽  
Diploid Cells

Available vaccines used in protection against rubella employ strains of virus attenuated by passage in animal cells. The first vaccine that was introduced in Britain, the Cendehill vaccine (Cendevax),1 is grown on tissue culture of rabbit kidney cells. Recently another rubella vaccine (Almevax live attenuated, Wistar RA 27/3), grown on human diploid cells, has been introduced. This vaccine induces antibodies as effectively as earlier vaccines, and the indications for its use are similar to those for the Cendehill vaccine.1 2 It is very important that pregnant women, women who might be pregnant, or women likely to become pregnant within 2 months, must not be vaccinated.

Induction of Tolerance to Skin Grafts in Mice with Disrupted Liver and Kidney Cells.

1964 ◽  
Vol 115 (1) ◽  
pp. 8-11 ◽  
Author(s):  
W. D. Kelly ◽  
J. M. Smith ◽  
C. Martinez ◽  
R. A. Good
Keyword(s):  
Kidney Cells ◽  
Skin Grafts ◽  
Liver And Kidney ◽  
Induction Of Tolerance

scholarly journals Su1209 Protective and Reparative Effect of Petadecapeptide BPC 157 on Mice Blood, Liver and Kidney Cells

2012 ◽  
Vol 142 (5) ◽  
pp. S-451
Author(s):  
Ivan Sopf ◽  
Nevenka Kopjar ◽  
Mirta Milic ◽  
Damir Sirovina ◽  
Sandro Mise ◽  
...  
Keyword(s):  
Kidney Cells ◽  
Bpc 157 ◽  
Liver And Kidney ◽  
Reparative Effect

scholarly journals The effects of added purines on urate and purine synthesis de novo by isolated chick liver, kidney and lymphoid cells

1976 ◽  
Vol 158 (3) ◽  
pp. 549-556 ◽  
Author(s):  
P Badenoch-Jones ◽  
P J Buttery
Keyword(s):  
De Novo ◽  
Cell Types ◽  
Liver Cells ◽  
Lymphoid Cells ◽  
Kidney Cells ◽  
Purine Synthesis ◽  
Liver And Kidney ◽  
Stimulation Of

1. Isolated chick lymphoid cells, together with isolated chick liver and kidney cells, incorporate [1-14C]glycine or [14C]formate into urate. 2. Of the cell types used, bursal cells incorporate 14C into urate at the fastest rate, although the output of total urate by bursal cells is only 10% that of liver cells. 3. When suspended in Eagle's medium the incorporation of 14C into urate is inhibited by adenine and guanine up to 1 mM. In contrast, the addition of 1 mM-AMP or -GMP results in a relatively large stimulation of this incorporation. 4. Added adenine is rapidly taken up by liver cells and then released in an unmetabolized form; AMP is taken up more slowly and is rapidly metabolized. The metabolites (possibly including adenine) are then released. 5. Intracellular liver 5-phosphoribosyl 1-pyrophosphate is approx. 0.7mM and remains constant or falls slightly during a 3 h incubation of the cells. 6. The addition of adenine or guanine, AMP or GMP, does not alter liver intracellular 5-phosphoribosyl 1-pyrophosphate concentrations. Added 5-phosphoribosyl 1-pyrophosphate is not taken up by liver cells. 7. The results are discussed in the context of the control of urate and purine synthesis de novo in the chick.

scholarly journals SYNCHRONIZATION OF MITOCHONDRIAL DNA SYNTHESIS IN CHINESE HAMSTER CELLS (LINE CHO) DEPRIVED OF ISOLEUCINE

1973 ◽  
Vol 58 (2) ◽  
pp. 340-345 ◽  
Author(s):  
Kenneth D. Ley ◽  
Marilyn M. Murphy
Keyword(s):  
Cell Cycle ◽  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Cell Cycle Progression ◽  
Time Course ◽  
Nuclear Dna ◽  
Tritiated Thymidine ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
In Cells

Mitochondrial DNA (mit-DNA) synthesis was compared in suspension cultures of Chinese hamster cells (line CHO) whose cell cycle events had been synchronized by isoleucine deprivation or mitotic selection. At hourly intervals during cell cycle progression, synchronized cells were exposed to tritiated thymidine ([3H]TdR), homogenized, and nuclei and mitochondria isolated by differential centrifugation. Mit-DNA and nuclear DNA were isolated and incorporation of radioisotope measured as counts per minute ([3H]TdR) per microgram DNA. Mit-DNA synthesis in cells synchronized by mitotic selection began after 4 h and continued for approximately 9 h. This time-course pattern resembled that of nuclear DNA synthesis. In contrast, mit-DNA synthesis in cells synchronized by isoleucine deprivation did not begin until 9–12 h after addition of isoleucine and virtually all [3H]TdR was incorporated during a 3-h interval. We have concluded from these results that mit-DNA synthesis is inhibited in CHO cells which are arrested in G1 because of isoleucine deprivation and that addition of isoleucine stimulates synchronous synthesis of mit-DNA. We believe this method of synchronizing mit-DNA synthesis may be of value in studies of factors which regulate synthesis of mit-DNA.

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