Induction of Tolerance to Skin Grafts in Mice with Disrupted Liver and Kidney Cells.

1. Isolated chick lymphoid cells, together with isolated chick liver and kidney cells, incorporate [1-14C]glycine or [14C]formate into urate. 2. Of the cell types used, bursal cells incorporate 14C into urate at the fastest rate, although the output of total urate by bursal cells is only 10% that of liver cells. 3. When suspended in Eagle's medium the incorporation of 14C into urate is inhibited by adenine and guanine up to 1 mM. In contrast, the addition of 1 mM-AMP or -GMP results in a relatively large stimulation of this incorporation. 4. Added adenine is rapidly taken up by liver cells and then released in an unmetabolized form; AMP is taken up more slowly and is rapidly metabolized. The metabolites (possibly including adenine) are then released. 5. Intracellular liver 5-phosphoribosyl 1-pyrophosphate is approx. 0.7mM and remains constant or falls slightly during a 3 h incubation of the cells. 6. The addition of adenine or guanine, AMP or GMP, does not alter liver intracellular 5-phosphoribosyl 1-pyrophosphate concentrations. Added 5-phosphoribosyl 1-pyrophosphate is not taken up by liver cells. 7. The results are discussed in the context of the control of urate and purine synthesis de novo in the chick.

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Possible Recovery of Manifestation of Prolactin Receptor and Some of Its Target Proteins in the Liver and Kidney Cells of Female Rats after Relief of Cholestasis Complicated and Not Complicated by Hyperprolactinemia

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-015-2963-0 ◽

2015 ◽

Vol 159 (3) ◽

pp. 361-364 ◽

Cited By ~ 2

Author(s):

M. I. Aleksandrova ◽

N. S. Sirotina ◽

O. V. Smirnova

Keyword(s):

Prolactin Receptor ◽

Female Rats ◽

Kidney Cells ◽

Target Proteins ◽

Liver And Kidney

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Incorporation of tritiated thymidine into mitochondrial DNA of the liver and kidney cells of chickens and mice in tissue culture

Histochemistry and Cell Biology ◽

10.1007/bf00304312 ◽

1967 ◽

Vol 10 (4) ◽

pp. 305-308 ◽

Cited By ~ 13

Author(s):

T. Nagata ◽

O. Shibata ◽

T. Nawa

Keyword(s):

Tissue Culture ◽

Mitochondrial Dna ◽

Tritiated Thymidine ◽

Kidney Cells ◽

Liver And Kidney

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Regucalcin ameliorates Doxorubicin-induced cytotoxicity in Cos-7 kidney cells and translocates from the nucleus to the mitochondria

Bioscience Reports ◽

10.1042/bsr20211464 ◽

2021 ◽

Author(s):

Noor A Mohammed ◽

Israa Hakeem ◽

Nikolas J Hodges ◽

Francesco Michelangeli

Keyword(s):

Cell Death ◽

Cell Model ◽

Cancer Drug ◽

Kidney Cells ◽

Strongly Correlated ◽

Caspase 9 ◽

Membrane Blebbing ◽

Chemically Induced ◽

Anti Cancer ◽

Liver And Kidney

Doxorubicin (DOX) is a potent anti-cancer drug, which can have unwanted side-effects such as cardiac and kidney toxicity. A detailed investigation was undertaken of the acute cytotoxic mechanisms of DOX on kidney cells, using Cos-7 cells as kidney cell model. Cos-7 cells were exposed to DOX for a period of 24 hours over a range of concentrations and the LC50 was determined to be 7µM. Further investigations showed that cell death was mainly via apoptosis involving Ca2+ and caspase 9, in addition to autophagy. Regucalcin (RGN), a cytoprotective protein found mainly in liver and kidney tissues, was overexpressed in Cos-7 cells and shown to protect against DOX-induced cell death. Subcellular localization studies in Cos-7 cells showed RGN to be strongly correlated with the nucleus. However, upon treatment with DOX for 4 hours, which induced membrane blebbing in some cells, the localization appeared to be correlated more with the mitochondria in these cells. It is yet to be determined whether this translocation is part of the cytoprotective mechanism or a consequence of chemically-induced cell stress.

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