Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activities in the motoneurons of exercised mice

1970 ◽  
Vol 23 (2) ◽  
pp. 116-119 ◽  
Author(s):  
Jan Jonek ◽  
Janusz Konecki ◽  
Henryk Grzybek ◽  
Zbigniew Olkowski
1964 ◽  
Vol 21 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Joseph Osinchak

Supraoptic nuclei in the hypothalamus of rats were fixed for the electron microscope by vascular perfusion with solutions of glutaraldehyde followed by post fixation with osmium tetroxide. Cytochemical methods for detection of acid phosphatase and thiamine pyrophosphatase activity have been applied to glutaraldehyde-fixed frozen sections containing the neurosecretory cells. The enzyme activities have been localized to certain Golgi cisternae. Acid phosphatase activity is present in the large (0.4 µ to 1.0 µ) granules or dense bodies which are surrounded by a single limiting membrane; both features characterize these structures as lysosomes. Smaller (0.1 µ) granules also present in the perikarya are generally unreactive towards enzyme activity and resemble in form the neurosecretory granules in the neurohypophysis.


1971 ◽  
Vol 8 (3) ◽  
pp. 727-733
Author(s):  
R. B. KNOX ◽  
J. HESLOP-HARRISON

Acid phosphatase has been localized in the wall of the pollen grain of Crocus vernus Wulf at the electron-microscope level by a method using 2-naphthyl thiol phosphate as substrate in a simultaneous coupling reaction with fast blue BBN at pH 5.0, the product being given electron opacity by osmication. Activity was found to be concentrated mainly in the intine, and to be associated with ribbon-like or filamentous inclusions believed to be proteinaceous on the basis of other criteria. Some activity was also detectable in the interstices of the exine. The observations confirm the general interpretation of the distribution of wall-held enzyme based upon light-microscopic cytochemistry, and provide the resolution necessary to establish unambiguously that they are associated with protein layers inserted during intine growth.


Pathology ◽  
1976 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
I.P. McCausland ◽  
R.N. Seelye ◽  
J.B. Gavin ◽  
P.B. Herdson

CHEST Journal ◽  
1973 ◽  
Vol 63 (4) ◽  
pp. 44S ◽  
Author(s):  
J.B. Richardson ◽  
J.C. Hogg ◽  
T. Bouchard ◽  
D.L. Hall

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